Procjena citotoksičnog i genotoksičnog utjecaja suvremenih dentalnih materijala in vivo

Objectives: The aim of the study was to assess the biocompatibility of modern composite and amalgam dental fillings. Material and Methods: The research was conducted on 150 healthy patients between the ages of 10 and 20 who had amalgam and composite fillings between 6 and 12 months. Under in vivo conditions, a swab of buccal cells near the fillings was taken, and the cytotoxic and genotoxic impact of composite and amalgam fillings on these cells was analyzed using the extended micronucleus test (cytomeassay). Results: The results showed statistically significant differences between the groups of subjects with amalgam and composite fillings and subjects without fillings for the following parameters: number of micronuclei (p=0.006), number of buds (p<0.001), number of binuclear cells (p<0.001), number of nucleoplasmic bridges (p<0.001).The number of micronuclei was statistically significantly higher in the group of subjects with amalgam and composite fillings compared to the group without fillings. The results for nuclear buds, for the number of binuclear cells and the number of nucleoplasmic bridges showed that the group with amalgam fillings had a statistically significantly higher number of these changes compared to other groups.The results of the analysis of the relationship between the parameters of the micronucleus test and the number of amalgam and composite surfaces did not show statistically significant values. Parameters indicating cell cytotoxicity were not statistically significantly elevated in subjects with fillings.The results of the analysis of the influence of the patients’ lifestyle on the results of the micronucleus test showed statistically significant results for certain predictors (diagnostic X-ray radiation, coffee consumption, consumption of cooked, dried meat and baked food). Conclusion: Based on the results, it can be concluded that the buccal cells of subjects with amalgam fillings showed the highest degree of genotoxic changes, followed by those with composite fillings and the least buccal cells of patients without fillings.Svrha rada: Željela se procijeniti biokompatibilnost suvremenih kompozitnih i amalgamskih zubnih ispuna. Materijali i postupci: Istraživanje je provedeno na skupini od 150 zdravih pacijenata u dobi između 10 i 20 godina koji su imali amalgamske i kompozitne ispune starosti između 6 i 12 mjeseci. U uvjetima in vivo uzimao se bris bukalnih stanica u blizini ispuna te se primjenom proširenoga mikronukleusnoga testa (cytomeassay) analizirao citotoksični i genotoksični utjecaj kompozitnih i amalgamskih ispuna na te stanice. Rezultati: Rezultati su pokazali statistički značajne razlike između skupina ispitanika s amalgamskim i kompozitnim ispunima te ispitanika bez ispuna za sljedeće parametre: broj mikronukleusa (p = 0,006), broj pupova (p < 0,001), broj binuklearnih stanica (p < 0,001) i broj nukleoplazmatskih mostova (p < 0,001). Broj mikronukleusa bio je statistički značajno veći u skupini ispitanika s amalgamskim i kompozitnim ispunima u usporedbi sa skupinom bez ispuna. Rezultati za jezgrene pupove, za broj binuklearnih stanica i broj nukleoplazmatskih mostova pokazali su da je skupina s amalgamskim ispunima imala statistički značajno veći broj tih promjena u usporedbi s ostalim skupinama. Rezultati analize povezanosti parametara mikronukleusnog testa i broja amalgamskih te kompozitnih ploha nisu pokazali statistički značajne vrijednosti. Parametri koji upozoravaju na citotoksičnost stanica nisu bili statistički znatno povišeni kod ispitanika s ispunima. Rezultati analize utjecaja načina života pacijenata na ishode mikronukleusnog testa pokazali su statistički značajne rezultate za određene prediktore (dijagnostičko rendgensko zračenje, konzumacija kave, kuhanih i pečenih jela te suhomesnatih proizvoda). Zaključak: Na temelju rezultata može se zaključiti da su bukalne stanice ispitanika s amalgamskim ispunima pokazale najviši stupanj genotoksičnih promjena, zatim slijede one s kompozitnim ispunima, a najmanje su promjene zabilježene kod pacijenata bez ispuna

Assessment of Cytotoxic and Genotoxic Effect of Modern Dental Materials in vivo

Objectives: The aim of the study was to assess the biocompatibility of modern composite and amalgam dental fillings. Material and Methods: The research was conducted on 150 healthy patients between the ages of 10 and 20 who had amalgam and composite fillings between 6 and 12 months. Under in vivo conditions, a swab of buccal cells near the fillings was taken, and the cytotoxic and genotoxic impact of composite and amalgam fillings on these cells was analyzed using the extended micronucleus test (cytomeassay). Results: The results showed statistically significant differences between the groups of subjects with amalgam and composite fillings and subjects without fillings for the following parameters: number of micronuclei (p=0.006), number of buds (p<0.001), number of binuclear cells (p<0.001), number of nucleoplasmic bridges (p<0.001).The number of micronuclei was statistically significantly higher in the group of subjects with amalgam and composite fillings compared to the group without fillings. The results for nuclear buds, for the number of binuclear cells and the number of nucleoplasmic bridges showed that the group with amalgam fillings had a statistically significantly higher number of these changes compared to other groups.The results of the analysis of the relationship between the parameters of the micronucleus test and the number of amalgam and composite surfaces did not show statistically significant values. Parameters indicating cell cytotoxicity were not statistically significantly elevated in subjects with fillings.The results of the analysis of the influence of the patients’ lifestyle on the results of the micronucleus test showed statistically significant results for certain predictors (diagnostic X-ray radiation, coffee consumption, consumption of cooked, dried meat and baked food). Conclusion: Based on the results, it can be concluded that the buccal cells of subjects with amalgam fillings showed the highest degree of genotoxic changes, followed by those with composite fillings and the least buccal cells of patients without fillings