Rank Optimization for MIMO systems with RIS: Simulation and Measurement

Reconfigurable intelligent surface (RIS) is a promising technology that can reshape the electromagnetic environment in wireless networks, offering various possibilities for enhancing wireless channels. Motivated by this, we investigate the channel optimization for multiple-input multiple-output (MIMO) systems assisted by RIS. In this paper, an efficient RIS optimization method is proposed to enhance the effective rank of the MIMO channel for achievable rate improvement. Numerical results are presented to verify the effectiveness of RIS in improving MIMO channels. Additionally, we construct a 2$\times$2 RIS-assisted MIMO prototype to perform experimental measurements and validate the performance of our proposed algorithm. The results reveal a significant increase in effective rank and achievable rate for the RIS-assisted MIMO channel compared to the MIMO channel without RIS

Derivation of Rabbit Embryonic Stem Cells from Vitrified–Thawed Embryos

The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified?thawed (V/T) embryos. We demonstrate that V/T blastocysts (BLs) can be used to derive pluripotent rbESCs with efficiencies comparable to those using fresh BLs. These ESCs are undistinguishable from the ones derived from fresh embryos. We tested the developmental capacity of rbESCs derived from V/T embryos by BL injection experiments and produced chimeric kits. Our work adds cryopreservation to the toolbox of rabbit stem cell research and applications and will greatly expand the available research materials for regenerative medicine in a clinically relevant animal model.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/140328/1/cell.2015.0044.pd

Recombinant Rabbit Leukemia Inhibitory Factor and Rabbit Embryonic Fibroblasts Support the Derivation and Maintenance of Rabbit Embryonic Stem Cells

Abstract The rabbit is a classical experimental animal species. A major limitation in using rabbits for biomedical research is the lack of germ-line-competent rabbit embryonic stem cells (rbESCs). We hypothesized that the use of homologous feeder cells and recombinant rabbit leukemia inhibitory factor (rbLIF) might improve the chance in deriving germ-line-competent rbES cells. In the present study, we established rabbit embryonic fibroblast (REF) feeder layers and synthesized recombinant rbLIF. We derived a total of seven putative rbESC lines, of which two lines (M5 and M23) were from culture Condition I using mouse embryonic fibroblasts (MEFs) as feeders supplemented with human LIF (hLIF) (MEF+hLIF). Another five lines (R4, R9, R15, R21, and R31) were derived from Condition II using REFs as feeder cells supplemented with rbLIF (REF+rbLIF). Similar derivation efficiency was observed between these two conditions (8.7% vs. 10.2%). In a separate experiment with 2?3 factorial design, we examined the effects of feeder cells (MEF vs. REF) and LIFs (mLIF, hLIF vs. rbLIF) on rbESC culture. Both Conditions I and II supported satisfactory rbESC culture, with similar or better population doubling time and colony-forming efficiency than other combinations of feeder cells with LIFs. Rabbit ESCs derived and maintained on both conditions displayed typical ESC characteristics, including ESC pluripotency marker expression (AP, Oct4, Sox2, Nanog, and SSEA4) and gene expression (Oct4, Sox2, Nanog, c-Myc, Klf4, and Dppa5), and the capacity to differentiate into three primary germ layers in vitro. The present work is the first attempt to establish rbESC lines using homologous feeder cells and recombinant rbLIF, by which the rbESCs were derived and maintained normally. These cell lines are unique resources and may facilitate the derivation of germ-line-competent rbESCs.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/98439/1/cell%2E2012%2E0001.pd

Identification of forensically important sarcophagid flies (Diptera: Sarcophagidae) based on COI gene in China

Abstract: Identifing an insect specimen is a crucial step in forensic entomology. As the stages and species of insect discovered on a corpse, such as Calliphoridae and Sarcophagidae, provides evidence for estimation of postmortem interval (PMI). However, morphologically distinguish may on occasion be impossible to the adult flies and nymphs of the same genus. A molecular method used the cytochrome oxidase subunits one (COI) sequence on mitochondrial DNA was established for sarcophagid species identification. In this study, a 272 base pair region of mitochondrial DNA (mtDNA) coding for COI was investigated for identification of the following forensically important sarcophagid flies. The specimens were from four families, including 8 Boerttcherisca Peregrina (Robineau -Desvoidy,1830) specimens of Boettcherisca, 2 Parasarcophaga similis (Meade, 1876) specimens, 4 Parasarcophaga albiceps (Meigen, 1826) and 8 Parasarcophaga dux (Thompson, 1869) specimens from Parasarcophaga. Phylogenetic analysis indicates that this partial COI region successfully identified all samples species to species group. Low levels of variation between some species indicate that sarcophagid flies from more locations should be studied in the future and local database set up are strongly recommended in China

Promoting biocompatibility of titanium by facile fabrication of phase-transited lysozyme induced hydroxyapatite coating

In this study, phase-transited lysozyme (PTL) induced Ca10(PO4)6(OH)2 (PTL-HA) coating was applied to the 3D-printed titanium surface for improving bioactivity. The morphology and phase of the coating were analyzed by EDS, SEM, XRD, and XPS, while biocompatibility was investigated in vitro. The observation from SEM showed that a uniform coating with the presence of spheric-like and plate-shaped crystals was covered on the titanium. The main composition of the coating was Ca10(PO4)6(OH)2. More importantly, the in vitro results showed the PTL-HA coating could upregulate the expression of osteogenic-related genes. Therefore, it is considered that the phase-transited lysozyme-induced hydroxyapatite film was a promising, rapid, low-cost, and green route to improve the bioactivity of a bone implant

Measurement-based small-scale channel model for sub-6 GHz RIS-assisted communications

Reconfigurable intelligent surfaces (RISs) have attracted increasing interest from both academia and industry, thanks to their unique features on controlling electromagnetic (EM) waves. Although theoretical models for RIS-empowered communications have covered a variety of applications, yet, very few papers have investigated the modeling of real propagation characteristics. In this paper, we fill this gap by providing an empirical statistical channel model to describe the small-scale channel variations for an RIS-assisted broadband system at 2.6 GHz. Based on real channel measurements in outdoor, indoor and outdoor-to-indoor (O2I) environments, we compare and analyze the global, inter-cluster and intra-cluster parameters. Measurement results indicate that the deployment of an RIS with proper phase configurations can significantly improve the channel quality by enhancing the K -factor and reducing the time dispersion. The small-scale fading is well characterized by the proposed statistical model and the empirical channel parameters. These results are essential for the design of emerging RIS-assisted wireless systems for future applications.<br/

Multi-Scenario Broadband Channel Measurement and Modeling for Sub-6 GHz RIS-Assisted Wireless Communication Systems

Reconfigurable intelligent surface (RIS)-empowered communication, has been considered widely as one of the revolutionary technologies for next generation networks. However, due to the novel propagation characteristics of RISs, underlying RIS channel modeling and measurement research is still in its infancy and not fully investigated. In this paper, we conduct multi-scenario broadband channel measurements and modeling for RIS-assisted communications at the sub-6 GHz band. The measurements are carried out in three scenarios covering outdoor, indoor, and outdoor-to-indoor (O2I) environments, which suffer from non-line-of-sight (NLOS) propagation inherently. Three propagation modes including intelligent reflection with RIS, specular reflection with RIS and the mode without RIS, are taken into account in each scenario. In addition, considering the cascaded characteristics of RIS-assisted channel by nature, two modified empirical models including floating-intercept (FI) and close-in (CI) are proposed, which cover distance and angle domains. The measurement results rooted in 2096 channel acquisitions verify the prediction accuracy of these proposed models. Moreover, the propagation characteristics for RIS-assisted channels, including path loss (PL) gain, PL exponent, spatial consistency, time dispersion, frequency stationarity, etc., are compared and analyzed comprehensively. These channel measurement and modeling results may lay the groundwork for future applications of RIS-assisted communication systems in practice

Synthesis and Crystal Structure of the Layered Lanthanide Oxychlorides Ba3Ln2O5Cl2

Single crystals of a new family of layered lanthanide oxychlorides, Ba3Ln2O5Cl2 (Ln = Gd-Lu), have been synthesized from a molten barium flux. This family crystallizes in the space group I4/mmm (No. 139; Z = 2) with lattice parameters a = 4.3384(1)-4.4541(1) Å and c = 24.5108(7)-24.8448(9) Å. Ba3Ln2O5Cl2 phases are built up of two different blocks: a perovskite double layer of stoichiometry Ba2Ln2O5 formed by corner-connected LnO5 tetragonal bipyramids and a puckered rock-salt-like interlayer of composition BaCl2. A complete structural study along with bond-valence-sum calculations shows that, for lanthanides larger than gadolinium, the structure becomes unstable. Density functional theory calculations show that the valence-band edge is dominated by oxygen orbitals, whereas the conduction band forms from Ba 5d orbitals. The synthesis of this family suggests a route to other potential multianion phases

Interferon regulatory factor-5 in resident macrophage promotes polycystic kidney disease

BackgroundAutosomal dominant polycystic kidney disease is caused by genetic mutations in PKD1 or PKD2. Macrophages and their associated inflammatory cytokines promote cyst progression; however, transcription factors within macrophages that control cytokine production and cystic disease are unknown.MethodsIn these studies, we used conditional Pkd1 mice to test the hypothesis that macrophage-localized interferon regulatory factor-5 (IRF5), a transcription factor associated with production of cyst-promoting cytokines (TNFα, IL-6), is required for accelerated cyst progression in a unilateral nephrectomy (1K) model. Analyses of quantitative real-time PCR (qRT-PCR) and flow-cytometry data 3 weeks post nephrectomy, a time point before the onset of severe cystogenesis, indicate an accumulation of inflammatory infiltrating and resident macrophages in 1K Pkd1 mice compared with controls. qRT-PCR data from FACS cells at this time demonstrate that macrophages from 1K Pkd1 mice have increased expression of Irf5 compared with controls. To determine the importance of macrophage-localized Irf5 in cyst progression, we injected scrambled or IRF5 antisense oligonucleotide (ASO) in 1K Pkd1 mice and analyzed the effect on macrophage numbers, cytokine production, and renal cystogenesis 6 weeks post nephrectomy.ResultsAnalyses of qRT-PCR and IRF5 ASO treatment significantly reduced macrophage numbers, Irf5 expression in resident—but not infiltrating—macrophages, and the severity of cystic disease. In addition, IRF5 ASO treatment in 1K Pkd1 mice reduced Il6 expression in resident macrophages, which was correlated with reduced STAT3 phosphorylation and downstream p-STAT3 target gene expression.ConclusionsThese data suggest that Irf5 promotes inflammatory cytokine production in resident macrophages resulting in accelerated cystogenesis.<br/