Antioxidant, antibacterial, cytotoxic, and apoptotic activity of stem bark extracts of Cephalotaxus griffithii Hook. f

<p>Abstract</p> <p>Background</p> <p><it>Cephalotaxus </it>spp. are known to possess various therapeutic potentials. <it>Cephalotaxus griffithii</it>, however, has not been evaluated for its biological potential. The reason may be the remoteness and inaccessibility of the habitat where it is distributed. The main aim of this study was to: (1) evaluate multiple biological potentials of stem bark of <it>C. griffithii</it>, and (2) identify solvent extract of stem bark of <it>C. griffithii </it>to find the one with the highest specific biological activity.</p> <p>Methods</p> <p>Dried powder of stem bark of <it>C. griffithii </it>was exhaustively extracted serially by soaking in petroleum ether, acetone and methanol to fractionate the chemical constituents into individual fractions or extracts. The extracts were tested for total phenolic and flavonoid content, antioxidant (DPPH radical scavenging, superoxide radical scavenging, and reducing power models), antibacterial (disc diffusion assay on six bacterial strains), cytotoxic (MTT assay on HeLa cells), and apoptotic activity (fluorescence microscopy, DNA fragmentation assay, and flow cytometry on HeLa cells).</p> <p>Results</p> <p>Among the three extracts of stem bark of <it>C. griffithii</it>, the acetone extract contained the highest amount of total phenolics and flavonoids and showed maximum antioxidant, antibacterial, cytotoxic (IC₅₀of 35.5 ± 0.6 μg/ml; P < 0.05), and apoptotic (46.3 ± 3.6% sub-G0/G1 population; P < 0.05) activity, followed by the methanol and petroleum ether extracts. However, there was no significant difference observed in IC₅₀values (DPPH scavenging assay) of the acetone and methanol extracts and the positive control (ascorbic acid). In contrast, superoxide radical scavenging assay-based antioxidant activity (IC₅₀) of the acetone and methanol extracts was significantly lower than the positive control (P < 0.05). Correlation analysis suggested that phenolic and flavonoid content present in stem bark of <it>C. griffithii </it>extracts was responsible for the high antioxidant, cytotoxic, and apoptotic activity (P < 0.05).</p> <p>Conclusions</p> <p>Stem bark of <it>C. griffithii </it>has multiple biological effects. These results call for further chemical characterization of acetone extract of stem bark of <it>C. griffithii </it>for specific bioactivity.</p

Global wealth disparities drive adherence to COVID-safe pathways in head and neck cancer surgery

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COMPUTED-TOMOGRAPHY IN THE PREOPERATIVE PLANNING OF ORAL ENDO-OSSEOUS IMPLANT-SURGERY

Computed tomography (CT) is a well-established aid in the preoperative assessment of the dimension of mandible and maxilla for endo-osseous implant installation. CT is a valuable tool for the measurement of the alveolar ridge and recognition of the course of the inferior alveolar nerve canal. In the present study, three different techniques were examined: normal CT images with coronal and sagittal slices, standard reconstructions based on axial slices, and multiplanar reconstruction and display (MPR/MPD), also based on axial slices. Six dissected human jaw bones were examined with these three techniques. Afterwards, these jaws were sawn, and the real values were measured. Comparing these scores with the radiological measurements, the standard reconstruction technique seemed the most reliable method in the preoperative examination of the jaw bone quality and bone proportion.status: publishe

CT scan standard reconstruction technique for reliable jaw bone volume determination

Computed tomography can assist the surgeon in planning the exact three-dimensional positioning of endosseous implants in the jaw bone. This study examined the reliability of three CT techniques: direct imaging (normal coronal and sagittal slices), standard reconstruction (based on axial slices), and multiplanar reconstruction and display (also based on axial slices). The three imaging techniques were used to estimate the bone height, bone width, and maximal implant length in six dissected human jaw bones. These jaws were then sawed perpendicular to their axis to enable measurement of real values for comparison. The sagittal and coronal slices frequently were overestimated, especially in the canine and premolar regions. The mean absolute deviation was 1.4 mm. Standard reconstruction offered the most reliable cross-sectional images, with a mean absolute deviation of 0.5 mm. The multiplanar reconstruction and display technique frequently demonstrated underestimations (mean absolute deviation was 2.3 mm). The standard reconstruction technique seems to be the method of choice in preoperative radiographic examination of patients before implant placement.status: publishe

Computed tomography in the preoperative planning of oral endo-osseous implant surgery

Computed tomography (CT) is a well-established aid in the preoperative assessment of the dimension of mandible and maxilla for endo-osseous implant installation. CT is a valuable tool for the measurement of the alveolar ridge and recognition of the course of the inferior alveolar nerve canal. In the present study, three different techniques were examined: normal CT images with coronal and sagittal slices, standard reconstructions based on axial slices, and multiplanar reconstruction and display (MPR/MPD), also based on axial slices. Six dissected human jaw bones were examined with these three techniques. Afterwards, these jaws were sawn, and the real values were measured. Comparing these scores with the radiological measurements, the standard reconstruction technique seemed the most reliable method in the preoperative examination of the jaw bone quality and bone proportion.status: publishe

Technica standard di ricostruzione mediante TAC per una corretta determinazione del volume dell'osso mascellare

status: publishe