Analiza utjecaja faktora rizika na pojavnost subkliničkog mastitisa na nekim farmama mliječnih krava na istoku Alžira

In order to study the effect of various risk factors on the prevalence of subclinical mastitis, we studied the prevalence of subclinical mastitis in 104 dairy cows (416 udders) from 18 bovine herds in three wilayas in eastern of Algeria using the California mastitis test (CMT) combined with bacteriological analysis. The aim of this research was to determine the effect of 27 risk factors related to the animal, its environment and milking practices on prevalence, as well as the effects of breed, age, stage of lactation, housing and litter type, hygiene level, season, mil production, vacuum level and elimination of first jets on subclinical mastitis frequency. A survey was carried out to collect data on cow numbers, breeds, type of breeding, housing, litter and the level of hygiene in farms, the method of milking and milking practices. The CMT combined with bacteriological examination was performed to detect subclinical mastitis, the Chi 2 test was used to test for difference between the means. Prevalence values were: 24% by CMT vs 17% by bacteriology analysis. Concerning udders, we recorded a rate of 10% vs. 8.7% respectively. The results of the survey show that the Holstein breed is the most exploited and affected (P<0.05). The number of dairy cows varied from 3 to 20 cows. In this study, 56% of cows were raised on farms built of cinder blocks, which had a lower rate compared to farms built of wood and reeds (P<0.001). Animal housing consisting of a concrete floor 33% vs. 36% raised with straw bedding and were more affected (P<0.001), litter is removed only once a day for 13% of cows, which is a factor that predisposes cows to this disease. The frequency is higher during early and late compared to mid-lactation phases (P<0.05); low udder conformation and the right side of udders also had an impact on this disease alongside animal hygiene (P<0.05), and cattle producing more than 12 litres per day were more susceptible (P<0.05). However, the frequency of mastitis was only slightly modified by the lactation number of cows. High and low vacuum level of milking machines and pulsation frequency predisposed cows more to this disease (P<0.05). In this study, cows milked manually were significantly more affected (P<0.05) particularly in summer and the winter, showing high rates (P<0.05).Da bismo uočili učinak različitih faktora rizika na prevalenciju subkliničkog mastitisa, ispitali smo prevalenciju subkliničkog mastitisa u 104 (416 vimena) mliječnih krava iz 18 stada goveda koja se uzgajaju u tri provincije (wilaya) u istočnom Alžiru. Ispitivanje smo proveli pomoću kalifornijskog testa za mastitis (CMT) u kombinaciji s bakteriološkom analizom. Cilj je ovog istraživanja bio utvrditi učinak 27 faktora rizika povezanih sa životinjama, njihovim okolišem i praksom mužnje na prevalenciju, kao i učinak pasmine, dobi, faze laktacije, vrste staje i stelje, razine higijene, godišnjeg doba, proizvodnje mlijeka, razine vakuuma i eliminacije prvih mlazova na učestalost subkliničkog mastitisa. Provedena je anketa u svrhu prikupljanja podataka o broju krava, eksploatiranim pasminama, vrsti uzgoja, staje, stelje i razini higijene na farmama, metodi mužnje i praksom mužnje. Proveden je CMT test u kombinaciji s bakteriološkim testiranjem za detekciju subkliničkog mastitisa. Chi 2 test je rabljen da bi se provjerila razlika između sredstava. Vrijednosti prevalencije bile su: 24 % prema CMT-u u usporedbi sa 17 % prema bakteriološkoj analizi. U svezi s vimenima, zabilježili smo stopu od 10 % u usporedbi s 8,7 %. Rezultati ankete pokazali su da je holstein pasmina najeksploatiranija i najviše zahvaćena (P<0,05); broj mliječnih krava varira od 3 do 20 krava. U ovoj studiji 56 % krava uzgajano je na farmama izgrađenima od opeke i one su imale najniže stope mastitisa u usporedbi s farmama izgrađenima od drva i trske (P<0,001). Staje za životinje imaju betonski pod za 33 % (životinja) u usporedbi s 36 % uzgajanih na podlozi od slame koje su bile više zahvaćene (P<0,001); stelja se podiže jednom na dan za 13 % krava, a to je uvjetovalo krave na ovu bolest. U usporedbi sa sredinom laktacije (P<0,05) učestalost je veća u ranoj i kasnoj laktaciji, niska morfologija vimena i desna strana vimena su utjecale su i na ovu bolest, uz higijenu životinja (P<0,05). Krave koje su proizvodile više od 12 L/dan bile su prijemčivije na bolest (P<0,05). Međutim, učestalost mastitisa malo je modificirana brojem laktacija krava. Visoki i niski vakuum strojeva za mužnju, učestalost impulsa više je uvjetovalo krave na ovu bolest (P<0,05). U ovoj studiji, krave koje su ručno mužene značajno su više bile pogođene ovom bolesti (P<0,05), posebice ljeti i zimi, pokazujući veće stope bolesti (P<0,05)

Influence of Three Probiotics Strains, Lactobacillus rhamnosus GG, Bifidobacterium animalis subsp. Lactis BB-12 and Saccharomyces boulardii CNCM I-745 on the Biochemical and Haematological Profiles and Body Weight of Healthy Rabbits

Currently, probiotics are used as growth promoters on a large scale to improve the productivity of several animals’ species within the aim of reducing the presence of antibiotic residues in animal products consumed by humans. Several reports evidenced the positive effect of probiotic supplementation on the growth performances and health of rabbits, mainly through the balance of the intestinal microbiota of the host animal. Therefore, certain probiotics, including Lactobacilli, Bifidobacteria, Saccharomyces, can improve the biochemical and haematological profiles, especially in production animals. In this context, this study was performed on rabbits for the economic importance they play as a source of meat proteins in developing countries and their use as experimental models in research and biomedicine. This study then aimed to evaluate the effect of three strains of probiotics: Lactobacillus rhamnosus GG Bifidobacterium animalis subsp. Lactis BB-12 and Saccharomyces boulardii CNCM I-745, on the biochemical and haematological parameters and their influence on the rabbit’s weight of the ITELV2006 strain. The findings evidenced that the probiotic strain affected the biochemical and haematological parameters. Further, the strains showed a positive effect on the weight gain of the rabbits.This study aimed to investigate the effects of three strains of probiotics, these being Lactobacillus rhamnosus GG, Bifidobacterium animalis subsp. Lactis BB-12 and Saccharomyces boulardii CNCM I-745, on the body weight, animal performances and blood parameters of rabbits (male and female) of the ITELV2006 strain. The supplementation of the feed of the rabbits with the three probiotic strains allowed observing positive effects on most of the biochemical and haematological parameters investigated during a period of 60 days (30 days of supplementation and 30 days without treatment). Further, there was a significant improvement in the body weight of the rabbits at the end of the experiment. The effect of the three probiotics investigated in this trial was found to be related to the sex of the rabbits and to the intake period (duration). Ultimately, these findings raise the possibility of using probiotics to investigate in an in-depth and specific manner based on fixed factors such as the strain, the gender and age of the animals, the main underlying mechanisms and effects, which would allow achieving optimal and adapted health benefits and sustainable production. In the context of animal production, it is worth investigating in a targeted study the effect of the three strains on muscle growth and development and finding evidence of the possible consequences on meat quality traits of the rabbits supplemented with probiotics

Preliminary investigation of the antimicrobial and mechanisms of resistance of Enterobacteria isolated from minced meat in the Northeast of Algeria: The case of butchers from Constantine

peer-reviewedFood products of animal origin such as fresh meat are easily contaminated by microorganisms if handling, processing and storage conditions are not fully respected. The present study aimed first to evaluate the bacterial load and microbial contamination rates of ground raw beef to identify the main pathogenic flora that dominate and second, to determine the resistance patterns and extended-spectrum beta-lactamase (ESBL) of isolated Gram-negative strains against certain families of antibiotics. Therefore, 39 samples have been collected from 5 butcher shops located in Constantine province in the North-East of Algeria. The samples were analysed for total bacterial count, presence of total and faecal coliforms, Staphylococci and Salmonella. Furthermore, 23 antibiotics were tested using the diffusion method on Mueller-Hinton agar, towards 22 strains isolates. Bacterial analyses showed a high contamination by total aerobic bacteria, total and faecal coliforms. Escherichia coli, Citrobacter spp., Enterobacter spp., Hafnia alvei, Salmonella pullorum and Staphylococcus spp (except Staphylococcus aureus) were further revealed in some samples. The results of the antibiogram test exhibit multi-resistance to more than eight antibiotics with varied effects. From the whole tested strains isolates, the fully susceptibility effect was for spectinomycin (SPT). This study reveals that the analysed minced meat was found to be highly contaminated with antibiotic resistant bacteria. This study allows concluding that appropriate use of antibiotics in compliance with good hygiene practices is essential to reduce the antibiotic resistance identified in this preliminary study

Feeding practices of dairy cows in Algeria: Characterization, typology, and impact on milk production and fertility

Objective: To explore feeding strategies and identify eventual errors that could cause poor pro-duction and reproduction performances in dairy farms in the north of Algeria.Materials and Methods: A survey was conducted among 211 farms of different sizes, from 2014 to 2018 to compose a database that is analyzed statistically.Results: The results relative to the nutritive value of the rations showed an average dry matter intake of 15.9 ± 4.74 kg/cow/day, providing 13.2 ± 4.34 UFL (Feed Unit for Lactation) of net energy and 1306 ± 456 gm of protein digested in small intestine (PDI)/cow/day. A high proportion of concentrate intake is observed, with an average of 64.7% ± 17.4% of energy intake and 70.2% ± 16.2% of nitrogen intake.Dairy cows performances were characterized by a low milk productionregarding their genetic potential (14.2 ± 4.73 kg of milk/cow/day), a calving interval > to one year (397 ± 20.4 days), though the coverage rates of their nutrient requirements reached 120%.The typology of the dietary rations allowed grouping them into three categories: deficient rations cluster (DR), correct rations cluster, and excessive rations cluster (ER). DR and ER, which are not adjusted to animals’ needs, were found in 57.8% of farms.Conclusion: It is obvious that the feeding management in the dairy farms in the North of Algeria is not based upon scientific achievements, contributing to relatively low performances of cows as well as to important financial losses. This finding imposes the necessity to adopt a correct and accurate rationing of animals

Study of Probiotic Effects of Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus plantarum 299v Strains on Biochemical and Morphometric Parameters of Rabbits after Obesity Induction

This study aimed first to develop an experimental model of obesity and metabolic syndrome over 14 weeks using a diet called “cafeteria”, which is a high-fat diet, to evaluate its consequences on the biochemical and morphometric parameters in ITELV2006 strain rabbits. Second, the trial aimed to evaluate the effect of two strains of probiotics, these being Bifidobacterium animalis subsp. lactis BB-12® and Lactobacillus plantarum 299v®, on the obesity and MetS induced during the first experiment. Overall, the results of the “cafeteria” diet demonstrated significant changes in numerous biochemical and morphometric parameters, reproducing obesity and the main clinical manifestations of the metabolic syndrome in humans. The administration of the two probiotic strains demonstrated an impact on certain parameters of obesity and induced MetS. This study makes it possible to conclude that probiotics could be useful in the treatment of obesity and metabolic syndrome of rabbits, but in a dependent manner. Furthermore, this study evidenced the importance of selecting specific probiotic strains and dosages to achieve desirable results on rabbits or other species

Study of Probiotic Effects of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> BB-12 and <i>Lactobacillus plantarum</i> 299v Strains on Biochemical and Morphometric Parameters of Rabbits after Obesity Induction

This study aimed first to develop an experimental model of obesity and metabolic syndrome over 14 weeks using a diet called “cafeteria”, which is a high-fat diet, to evaluate its consequences on the biochemical and morphometric parameters in ITELV2006 strain rabbits. Second, the trial aimed to evaluate the effect of two strains of probiotics, these being Bifidobacterium animalis subsp. lactis BB-12® and Lactobacillus plantarum 299v®, on the obesity and MetS induced during the first experiment. Overall, the results of the “cafeteria” diet demonstrated significant changes in numerous biochemical and morphometric parameters, reproducing obesity and the main clinical manifestations of the metabolic syndrome in humans. The administration of the two probiotic strains demonstrated an impact on certain parameters of obesity and induced MetS. This study makes it possible to conclude that probiotics could be useful in the treatment of obesity and metabolic syndrome of rabbits, but in a dependent manner. Furthermore, this study evidenced the importance of selecting specific probiotic strains and dosages to achieve desirable results on rabbits or other species