1,978 research outputs found
A tight coupling between β\u3csub\u3e2\u3c/sub\u3eY97 and β\u3csub\u3e2\u3c/sub\u3eF200 of the GABA\u3csub\u3eA\u3c/sub\u3e receptor mediates GABA binding
The GABAA receptor is an oligopentameric chloride channel that is activated via conformation changes induced upon the binding of the endogenous ligand, GABA, to the extracellular inter-subunit interfaces. Although dozens of amino acid residues at the α/β interface have been implicated in ligand binding, the structural elements that mediate ligand binding and receptor activation are not yet fully described. In this study, double-mutant cycle analysis was employed to test for possible interactions between several arginines (α1R67, α1R120, α1R132, and β2R207) and two aromatic residues (β2Y97 and β2F200) that are present in the ligand-binding pocket and are known to influence GABA affinity. Our results show that neither α1R67 nor α1R120 is functionally coupled to either of the aromatics, whereas a moderate coupling exists between α1R132 and both aromatic residues. Significant functional coupling between β2R207 and both β2Y97 and β2F200 was found. Furthermore, we identified an even stronger coupling between the two aromatics, β2Y97 and β2F200, and for the first time provided direct evidence for the involvement of β2Y97 and β2F200 in GABA binding. As these residues are tightly linked, and mutation of either has similar, severe effects on GABA binding and receptor kinetics, we believe they form a single functional unit that may directly coordinate GABA
Population Genetic Structuring in Opisthorchis viverrini over Various Spatial Scales in Thailand and Lao PDR
Khon Kaen Province in northeast Thailand is known as a hot spot for opisthorchiasis in Southeast Asia. Preliminary allozyme and mitochondrial DNA haplotype data from within one endemic district in this Province (Ban Phai), indicated substantial genetic variability within Opisthorchis viverrini. Here, we used microsatellite DNA analyses to examine the genetic diversity and population structure of O. viverrini from four geographically close localities in Khon Kaen Province. Genotyping based on 12 microsatellite loci yielded a mean number of alleles per locus that ranged from 2.83 to 3.7 with an expected heterozygosity in Hardy-Weinberg equilibrium of 0.44-0.56. Assessment of population structure by pairwise F(ST) analysis showed inter-population differentiation (P<0.05) which indicates population substructuring between these localities. Unique alleles were found in three of four localities with the highest number observed per locality being three. Our results highlight the existence of genetic diversity and population substructuring in O. viverrini over a small spatial scale which is similar to that found at a larger scale. This provides the basis for the investigation of the role of parasite genetic diversity and differentiation in transmission dynamics and control of O. viverrini
Experimental Realization of an Exact Solution to the Vlasov Equations for an Expanding Plasma
We study the expansion of ultracold neutral plasmas in the regime in which
inelastic collisions are negligible. The plasma expands due to the thermal
pressure of the electrons, and for an initial spherically symmetric Gaussian
density profle, the expansion is self-similar. Measurements of the plasma size
and ion kinetic energy using fluorescence imaging and spectroscopy show that
the expansion follows an analytic solution of the Vlasov equations for an
adiabatically expanding plasma.Comment: 4 pages, 4 figure
Electron Temperature Evolution in Expanding Ultracold Neutral Plasmas
We have used the free expansion of ultracold neutral plasmas as a
time-resolved probe of electron temperature. A combination of experimental
measurements of the ion expansion velocity and numerical simulations
characterize the crossover from an elastic-collision regime at low initial
Gamma_e, which is dominated by adiabatic cooling of the electrons, to the
regime of high Gamma_e in which inelastic processes drastically heat the
electrons. We identify the time scales and relative contributions of various
processes, and experimentally show the importance of radiative decay and
disorder-induced electron heating for the first time in ultracold neutral
plasmas
Using Absorption Imaging to Study Ion Dynamics in an Ultracold Neutral Plasma
We report optical absorption imaging of ultracold neutral plasmas.Images are
used to measure the ion absorption spectrum, which is Doppler-broadened.
Through the spectral width, we monitor ion equilibration in the first 250ns
after plasma formation. The equilibration leaves ions on the border between the
weakly coupled gaseous and strongly coupled liquid states. On a longer
timescale of microseconds, we observe radial acceleration of ions resulting
from pressure exerted by the trapped electron gas.Comment: 4 pages, 4 figure
Ultracold Neutral Plasmas
Ultracold neutral plasmas are formed by photoionizing laser-cooled atoms near
the ionization threshold. Through the application of atomic physics techniques
and diagnostics, these experiments stretch the boundaries of traditional
neutral plasma physics. The electron temperature in these plasmas ranges from
1-1000 K and the ion temperature is around 1 K. The density can approach
cm. Fundamental interest stems from the possibility of
creating strongly-coupled plasmas, but recombination, collective modes, and
thermalization in these systems have also been studied. Optical absorption
images of a strontium plasma, using the Sr
transition at 422 nm, depict the density profile of the plasma, and probe
kinetics on a 50 ns time-scale. The Doppler-broadened ion absorption spectrum
measures the ion velocity distribution, which gives an accurate measure of the
ion dynamics in the first microsecond after photoionization.Comment: 12th International Congress on Plasma Physics, 25-29 October 2004,
Nice (France
Absorption Imaging and Spectroscopy of Ultracold Neutral Plasmas
Absorption imaging and spectroscopy can probe the dynamics of an ultracold
neutral plasma during the first few microseconds after its creation.
Quantitative analysis of the data, however, is complicated by the inhomogeneous
density distribution, expansion of the plasma, and possible lack of global
thermal equilibrium for the ions. In this article we describe methods for
addressing these issues. Using simple assumptions about the underlying
temperature distribution and ion motion, the Doppler-broadened absorption
spectrum obtained from plasma images can be related to the average temperature
in the plasma.Comment: 14 pages, 8 figure
Magnetic trapping of metastable atomic strontium
We report the magnetic trapping of metastable atomic strontium. Atoms
are cooled in a magneto-optical trap (MOT) operating on the dipole allowed
transition at 461 nm. Decay via
continuously loads a magnetic trap formed by the quadrupole magnetic field of
the MOT. Over atoms at a density of cm and
temperature of 1 mK are trapped. The atom temperature is significantly lower
than what would be expected from the kinetic and potential energy of atoms as
they are transferred from the MOT. This suggests that thermalization and
evaporative cooling are occurring in the magnetic trap.Comment: This paper has been accepted by PR
Phylogenetic relationships within the Opisthorchis viverrini species complex with specific analysis of O. viverrini sensu lato from Sakon Nakhon, Thailand by mitochondrial and nuclear DNA sequencing
The liver fluke Opisthorchis viverrini sensu lato causes serious public-health problems in Northeast Thailand and Southeast Asian countries. A hypothesis has been proposed that O. viverrini represents a species complex with varying levels of genetic differentiation in Thailand and Lao PDR. This study aimed to clarify whether O. viverrini populations can be genetically divided into separate taxa. We collected O. viverrini s.l. from eight different locations in Lao PDR and Thailand. The results of nad1, cox1, CF-int6, Pm-int9, ITS2 and 28S rDNA sequence analysis revealed that sub-structuring occurred between the eight populations. We found that O. viverrini s.l. from Sakon Nakhon (SK), Thailand, shows significant genetic differentiation (P < .05) from all other isolates from different localities in Thailand and Lao PDR. This was supported by haplotype and phylogenetic tree analyses in which the SK isolate was separated from all other isolates. This suggests that O. viverrini s.l. from SK is a cryptic species. The data, however, also confirm the association between genetic groups of O. viverrini s.l. and specific wetland systems, and raise important questions regarding the epidemiological significance of these genetic differences
Solution of the relativistic Dirac-Hulthen problem
The one-particle three-dimensional Dirac equation with spherical symmetry is
solved for the Hulthen potential. The s-wave relativistic energy spectrum and
two-component spinor wavefunctions are obtained analytically. Conforming to the
standard feature of the relativistic problem, the solution space splits into
two distinct subspaces depending on the sign of a fundamental parameter in the
problem. Unique and interesting properties of the energy spectrum are pointed
out and illustrated graphically for several values of the physical parameters.
The square integrable two-component wavefunctions are written in terms of the
Jacobi polynomials. The nonrelativistic limit reproduces the well-known
nonrelativistic energy spectrum and results in Schrodinger equation with a
"generalized" three-parameter Hulthen potential, which is the sum of the
original Hulthen potential and its square.Comment: 13 pages, 3 color figure
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