20 research outputs found
Tu1924 Identification of Glycoprotein 2 as an Immunomodulator of Innate and Adaptive Immune Responses
947 – Priming and Maturation of the Human Fetal Immunity Occurs Early in Fetal Development
W1632 Pattern Recognition Repeats Have Differential Effects On Innate Responses in the Normal and Inflamed Intestinal Mucosa
Identification of Pancreatic Glycoprotein 2 as an Endogenous Immunomodulator of Innate and Adaptive Immune Responses
W1214 The Chemokine Cxcl12 Has Constitutive and Inflammation-Induced Expression By Intestinal Epithelial Cells In Vivo, and Attracts Cxcr4+ Interferon-Gamma (IFN-Gamma) Secreting Mucosal Lymphocytes In Vitro
Antibodies to GP2, the major zymogen granule membrane glycoprotein, in inflammatory bowel diseases reply
Single-cell atlas of the human neonatal small intestine affected by necrotizing enterocolitis.
Necrotizing enterocolitis (NEC) is a gastrointestinal complication of premature infants with high rates of morbidity and mortality. A comprehensive view of the cellular changes and aberrant interactions that underlie NEC is lacking. This study aimed at filling in this gap. We combine single-cell RNA sequencing (scRNAseq), T-cell receptor beta (TCRβ) analysis, bulk transcriptomics, and imaging to characterize cell identities, interactions, and zonal changes in NEC. We find an abundance of proinflammatory macrophages, fibroblasts, endothelial cells as well as T cells that exhibit increased TCRβ clonal expansion. Villus tip epithelial cells are reduced in NEC and the remaining epithelial cells up-regulate proinflammatory genes. We establish a detailed map of aberrant epithelial-mesenchymal-immune interactions that are associated with inflammation in NEC mucosa. Our analyses highlight the cellular dysregulations of NEC-associated intestinal tissue and identify potential targets for biomarker discovery and therapeutics