Cellular origin and ultrastructure of membranes induced during poliovirus infection

Poliovirus RNA replicative complexes are associated with cytoplasmic membranous structures that accumulate during viral infection. These membranes were immunoisolated by using a monoclonal antibody against the viral nonstructural protein 2C. Biochemical analysis of the isolated membranes revealed that several organelles of the host cell (lysosomes, trans-Golgi stack and trans-Golgi network, and endoplasmic reticulum) contributed to the virus-induced membranous structures. Electron microscopy of infected cells preserved by high-pressure freezing revealed that the virus-induced membranes contain double lipid bilayers that surround apparently cytosolic material. Immunolabeling experiments showed that poliovirus proteins 2C and 3D were localized to the same membranes as the cellular markers tested. The morphological and biochemical data are consistent with the hypothesis that autophagy or a similar host process is involved in the formation of the poliovirus-induced membranes

Studying Anomalous WWγWW\gamma and WWZWWZ Couplings with Polarized ppˉp\bar{p} Collisions

We calculate tree-level cross-sections for $W^{+}\gamma$ and $W^{+}W^{-}$ production in proton-antiproton collisions, with one $W$ decaying to leptons, with anomalous electroweak triple-boson coupling parameters $\Delta\kappa$ and $\lambda$. We compare the unpolarized cross-sections to those for a polarized proton beam, to study how a polarized proton beam would improve experimental tests of anomalous couplings.Comment: 36 pages, 14 postscript figure

Microtubules in Bacteria: Ancient Tubulins Build a Five-Protofilament Homolog of the Eukaryotic Cytoskeleton

Microtubules play crucial roles in cytokinesis, transport, and motility, and are therefore superb targets for anti-cancer drugs. All tubulins evolved from a common ancestor they share with the distantly related bacterial cell division protein FtsZ, but while eukaryotic tubulins evolved into highly conserved microtubule-forming heterodimers, bacterial FtsZ presumably continued to function as single homopolymeric protofilaments as it does today. Microtubules have not previously been found in bacteria, and we lack insight into their evolution from the tubulin/FtsZ ancestor. Using electron cryomicroscopy, here we show that the tubulin homologs BtubA and BtubB form microtubules in bacteria and suggest these be referred to as “bacterial microtubules” (bMTs). bMTs share important features with their eukaryotic counterparts, such as straight protofilaments and similar protofilament interactions. bMTs are composed of only five protofilaments, however, instead of the 13 typical in eukaryotes. These and other results suggest that rather than being derived from modern eukaryotic tubulin, BtubA and BtubB arose from early tubulin intermediates that formed small microtubules. Since we show that bacterial microtubules can be produced in abundance in vitro without chaperones, they should be useful tools for tubulin research and drug screening

A Probe of New Physics in Top Quark Pair Production at e−e+e^-e^+ Colliders

We describe how to probe new physics through examination of the form factors describing the Ztt couplings via the scattering process e^-e^+->t+tbar. We focus on experimental methods on how the top quark momentum can be determined and show how this can be applied to select polarized samples of $t\bar{t}$ pairs through the angular correlations in the final state leptons. We also study the dependence on the energy and luminosity of an \ee\ collider to probe a CP violating asymmetry at the $10^{-2}$ level.}Comment: 24 pages in TeXsis (figures available upon request) (revised July 1993

Tracing CP violation in the production of top quark pairs by multiple TeV proton-proton collisions

We investigate the possibilities of searching for non-standard CP violation in $pp\to t\bar{t}X$ at multiple TeV collision energies. A general kinematic analysis of the underlying partonic production processes $gg\to t\bar{t}$ and $q\bar{q}\to t\bar{t}$ in terms of their density matrices is given. We evaluate the CP-violating parts of these matrices in two-Higgs doublet extensions of the standard model (SM) and give results for CP asymmetries at the parton level. We show that these asymmetries can be traced by measuring suitable observables constructed from energies and momenta of the decay products of $t$ and $\bar{t}$. We find CP-violating effects to be of the order of $10^{-3}$ and show that possible contaminations induced by SM interactions are savely below the expected signals.Comment: 24 pages, SLAC-PUB-6403, PITHA 93/43, 9 Figs. available upon request. Written in LaTe

Massive Lepton Pairs as a Prompt Photon Surrogate

We discuss the transverse momentum distribution for the production of massive lepton-pairs in hadron reactions at fixed target and collider energies within the context of next-to-leading order perturbative quantum chromodynamics. For values of the transverse momentum $Q_T$ greater than the pair mass $Q$, $Q_T > Q$, we show that the differential cross section is dominated by subprocesses initiated by incident gluons. Massive lepton-pair differential cross sections are an advantageous source of constraints on the gluon density, free from the experimental and theoretical complications of photon isolation that beset studies of prompt photon production. We compare calculations with data and provide predictions for the differential cross section as a function of $Q_T$ in proton-antiproton reactions at center-of-mass energies of 1.8 TeV, and in proton-nucleon reactions at fixed target and LHC energies.Comment: 36 pages, RevTeX, including 16 ps files of figures; minor changes in wording; one reference added. Version to appear in Phys Rev

The t W- Mode of Single Top Production

The t W- mode of single top production is proposed as an important means to study the weak interactions of the top quark. While the rate of this mode is most likely too small to be observed at Run II of the Fermilab Tevatron, it is expected to be considerably larger at the CERN LHC. In this article the inclusive t W- rate is computed, including O(1 / log (m_t^2 / m_b^2)) corrections, and when combined with detailed Monte Carlo simulations including the top and W decay products, indicate that the t W- single top process may be extracted from the considerable t tbar and W+ W- j backgrounds at low luminosity runs of the LHC.Comment: 16 pages, 4 figure

QCD corrections to decay-lepton polar and azimuthal angular distributions in e+e- -> t tbar in the soft-gluon approximation

QCD corrections to order alpha_s in the soft-gluon approximation to angular distributions of decay charged leptons in the process e+e- -> t tbar followed by semileptonic decay of t or tbar, are obtained in the e+e- centre-of-mass frame. As compared to distributions in the top rest frame, these have the advantage that they would allow direct comparison with experiment without the need to reconstruct the top rest frame. The results also do not depend on the choice of a spin quantization axis for t or tbar. Analytic expression for the triple distribution in the polar angle of t and polar and azimuthal angles of the lepton is obtained. Analytic expression is also derived for the distribution in the charged-lepton polar angle. Numerical values are discussed for total c.m. energies of 400 GeV, 800 GeV and 1500 GeV.Comment: 21 pages, Latex, 6 figures included in the submission. To appear in Pramana - Journal of Physics; expanded version of hep-ph/0011321, v

Electron Cryotomography of Bacterial Cells

While much is already known about the basic metabolism of bacterial cells, many fundamental questions are still surprisingly unanswered, including for instance how they generate and maintain specific cell shapes, establish polarity, segregate their genomes, and divide. In order to understand these phenomena, imaging technologies are needed that bridge the resolution gap between fluorescence light microscopy and higher-resolution methods such as X-ray crystallography and NMR spectroscopy