Aktiviti Antimikrob Ekstrak Vernonia Cinerea L., Tridax Procumbens L., Dan Emilia Sonchifolia L. Daripada Famili Asteraceae

This research was conducted to study the antimicrobial effect of three plant extracts of Asteraceae family namely Vernonia cinerea L., Tridax procumbens L., and Emilia sonchifolia L. Kajian ini telah dijalankan untuk mengkaji kesan aktiviti antimikrob tiga jenis tumbuhan daripada famili Asteraceae iaitu Vernonia cinerea L., Tridax procumbens L., dan Emilia sonchifolia L

Standardization of Euphorbia hirta with Chemical Compounds Identification (GC-MS)

The present study was intended to evaluate the various pharmacognostical procedures in the leaves of E. hirta, (Euphorbiaceae). The various pharmacognostical parameters were carried out as per WHO guidelines procedure i.e., macroscopic and microscopic evaluation, heavy metal analysis, functional group analysis by Fourier Transform Infrared (FTIR) spectroscopy and assay. The study was extended with analyzing the chemical compounds identification in the extract of E. hirta by using GC-MS. The assay part of standardization involved determination of the antioxidant activity (DPPH assay) which could help assesses the chemical effects and establishes curative values. The extracts showed an antioxidant activity above 50%, independent of the extraction time. Six major peaks in the range of 600 - 1500 and 2800 - 3400 cm-1 were observed in the FTIR spectra. The chemical constituent aspect of standardization involves quantification of the main chemical components in E. hirta. The GC-MS method used for quantification of 1,3,4,5-tetrahydroxycyclohexanecarboxylic acid in the extract was rapid, accurate, precise, linear (R2 = 0.9981), rugged and robust. The concentrations of heavy metals determined in E. hirta extract are well below the permissible limit. The result of the pharmacognostical standardization of this plant serves as a reference piece and helps in future identification and authentication of this plant specimen

The antimicrobial efficacy of elaeis guineensis : characterization, in Vitro and in Vivo studies

The urgent need to treat multi-drug resistant pathogenic microorganisms in chronically infected patients has given rise to the development of new antimicrobials from natural resources. We have tested Elaeis guineensis Jacq (Arecaceae) methanol extract against a variety of bacterial, fungal and yeast strains associated with infections. Our studies have demonstrated that E. guineensis exhibits excellent antimicrobial activity in vitro and in vivo against the bacterial and fungal strains tested. A marked inhibitory effect of the E. guineensis extracts was observed against C. albicans whereby E. guineensis extract at =, 1, or 2 times the MIC significantly inhibited C. albicans growth with a noticeable drop in optical density (OD) of the bacterial culture. This finding confirmed the anticandidal activity of the extract on C. albicans. Imaging using scanning (SEM) and transmission (TEM) electron microscopy was done to determine the major alterations in the microstructure of the extract-treated C. albicans. The main abnormalities noted via SEM and TEM studies were the alteration in morphology of the yeast cells. In vivo antimicrobial activity was studied in mice that had been inoculated with C. albicans and exhibited good anticandidal activity. The authors conclude that the extract may be used as a candidate for the development of anticandidal agent.<br /

Wound Healing Activity of Elaeis guineensis Leaf Extract Ointment

Elaeis guineensis of the Arecaceae family is widely used in the traditional medicine of societies in West Africa for treating various ailments. To validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied. The results showed that E. guineensis leaf extract had potent wound healing capacity as evident from the better wound closure (P < 0.05), improved tissue regeneration at the wound site, and supporting histopathological parameters pertaining to wound healing. Matrix metalloproteinases expression correlated well with the results thus confirming efficacy of E. guineensis in the treatment of the wound. E. guineensis accelerated wound healing in rats, thus supporting its traditional use. The result of this study suggested that, used efficiently, oil palm leaf extract is a renewable resource with wound healing properties

Cytotoxicity and Oral Acute Toxicity Studies of Lantana camara Leaf Extract

Background: The objective of this study was to investigate the toxicity of Lantana camara methanol extract. Methods: In order to evaluate the toxicity of Lantana camara, the acute toxicity of the methanolic extract on adult mice and cytotoxicity test on Vero cell line were investigated. A fixed large dose of 2 g/kg body weight of L. camara leaf extract was administrated by a single oral gavage according to the OECD procedure. Results: In 2 weeks, L. camara leaf extract showed no obvious acute toxicity. While female mice lost body weight after being treated with single dose of leaf extract in acute toxicity test, male ones lost organ mass, particularly for heart and kidney. The biochemical liver function tests showed significantly elevated TBIL and ALT in the L. camara leaf extract treated female mice group compared with the control group. Cytotoxicity effect of leaf extract of L. camara was estimated through a MTT assay. Cytotoxicity tests on Vero cell line disclosed that leaf extract at concentrations up to 500 µg/mL inhibited the growth of cells 2.5 times less than did Triton 100× 1%. More interestingly, the cytotoxicity initiated to decline at elevated concentrations of this extract. Conclusions: The results of both tests confirm that L. camara shows a pro toxic effect

Standardization of Cassia Spectabilis with respect to authenticity, assay and chemical constituent analysis

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Ethical issues in the prevention of H1N1: the Malaysian experience

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