31 research outputs found
Addressing Catheter Associated UTI in the Neuro ICU
Increasing systemic pressures have forced clinicians to identify and address a variety of metrics to provide better “quality” care. Catheter associated UTIs (CAUTI) are a common health careassociated infection in the Neuro ICU and are associated with increased LOS and overall mortality.
Aims for Improvement: Improving the incidence of CAUTI at the Gibbon and JHN ICUs on an annual basis after incremental interventions
Improving Serial Imaging Protocols in Spontaneous Intracerebral Hemorrhage
There is no universally agreed upon protocol to image patient presenting with intra-parenchymal hemorrhage of non-traumatic etiology (sICH). At our institution, it is common practice for a patient to have 3 CT’s done within 24 hours. They are often at onset of symptoms or presentation, 6 hours post onset of symptoms, and finally 24 hours post bleed onset.
The goal of this project will be to assess the safety and efficacy of obtaining this repeat imaging in our patients in the hopes that limiting unnecessary CT head studies will decrease resource utilization, decrease patient radiation, expedite movement of stable patients out of the ICU and/or disposition
Standardizing Postoperative Handoffs Using the Evidence-Based IPASS Framework Improves Handoff Communication for Postoperative Neurosurgical Patients in the Neuro-Intensive Care Unit
Aims for Improvement
Within one year of initiation of the process improvement plan, we wanted to improve: Direct communication of airway and hemodynamic concerns Direct communication of operative events, complications, and perioperative management goals. Attendance at postoperative handoffs Confirmation of information by receiving teams Staff perceptions of handoff efficacy and teamwork
Identification and fragmentation pathways of caffeine metabolites in urine samples via liquid chromatography with positive electrospray ionization coupled to a hybrid quadrupole linear ion trap (LTQ) and Fourier transform ion cyclotron resonance mass spectrometry and tandem mass spectrometry.
Liquid chromatography (LC) with positive ion electrospray ionization (ESI+) coupled to a hybrid quadrupole linear ion trap (LTQ) and Fourier transform ion cyclotron resonance mass spectrometry (FTICRMS) was employed for the simultaneous determination of caffeine and its metabolites in human urine within a single chromatographic run. LC/ESI-FTICRMS led to the unambiguous determination of the molecular masses of the studied compounds without interference from other biomolecules. A systematic and comprehensive study of the mass spectral behaviour of caffeine and its fourteen metabolites by tandem mass spectrometry (MS/MS) was performed, through in-source ion trap collision-induced dissociation (CID) of the protonated molecules, [M+H](+). A retro-Diels-Alder (RDA) process along with ring-contraction reactions were the major fragmentation pathways observed during CID. The base peak of xanthine precursors originates from the loss of methyl isocyanate (CH(3)NCO, 57 Da) or isocyanic acid (HNCO, 43 Da), which in turn lose a CO unit. Also uric acid derivatives shared a RDA rearrangement as a common fragmentation process and a successive loss of CO(2) or CO. The uracil derivatives showed a loss of a ketene unit (CH(2)CO, 42 Da) from the protonated molecule along with the loss of H(2)O or CO. To assess the potential of the present method three established metabolite ratios to measure P450 CYP1A2, N-acetyltransferase and xanthine oxidase activities were evaluated by a number of identified metabolites from healthy human urine samples after caffeine intake
Electrospray ionizationÂŻtandem mass spectrometry of the Quorum Sensing peptide pheromones LamD produced by Lactobacillus Plantarum WCFS1
“FRAGMENTATION STUDY OF CAFFEINE AND ITS XANTHINE METABOLITES IN URINE SAMPLES BY LC-ESI-MS AND TANDEM MS”
Comunicazione POSTER
Tandem mass spectrometry investigation of Quorum Sensing peptide-precursors produced by Lactobacillus Plantarum by a hybrid mass spectrometric analyzer with low and high resolution
Fragmentation pathways of synthetic peptides investigated by infrared multiphoton dissociation (IRMPD) and Fourier-transform ion cyclotron mass spectrometry (FTICR MS)
Identification and fragmentation pathways of caffeine metabolites in urine samples via combined LC-ESI-LTQ-FTICRMS and tandem mass spectrometry
Liquid chromatography (LC) with positive ion electrospray ionization (ESIR) coupled to a hybrid
quadrupole linear ion trap (LTQ) and Fourier transform ion cyclotron resonance mass spectrometry
(FTICRMS) was employed for the simultaneous determination of caffeine and its metabolites in
human urine within a single chromatographic run. LC/ESI-FTICRMS led to the unambiguous
determination of the molecular masses of the studied compounds without interference from other
biomolecules. A systematic and comprehensive study of the mass spectral behaviour of caffeine and
its fourteen metabolites by tandem mass spectrometry (MS/MS) was performed, through in-source
ion trap collision-induced dissociation (CID) of the protonated molecules, [MRH]R. A retro-Diels-
Alder (RDA) process along with ring-contraction reactions were the major fragmentation pathways
observed during CID. The base peak of xanthine precursors originates from the loss of methyl
isocyanate (CH3NCO, 57 Da) or isocyanic acid (HNCO, 43 Da), which in turn lose a CO unit. Also uric
acid derivatives shared a RDA rearrangement as a common fragmentation process and a successive
loss of CO2 or CO. The uracil derivatives showed a loss of a ketene unit (CH2CO, 42 Da) from the
protonated molecule along with the loss of H2O or CO. To assess the potential of the present method
three established metabolite ratios to measure P450 CYP1A2, N-acetyltransferase and xanthine
oxidase activities were evaluated by a number of identified metabolites from healthy human urine
samples after caffeine intake