Atmospheric corrections of low altitude thermal infrared airborne images acquired over a tropical cropped area

Corrections atmosphériques d'images thermiques de cultures tropicales acquises à basse altitude. / Accurate corrections of atmospheric effects on thermal infrared remote sensing data are an essential pre-requisite for the development of thermal infrared airborne-derived crop water stress indices. These corrections can be performed using ground surface temperature measurements, which are time consuming and expensive. Atmospheric effects can also be corrected using radiative transfer models that require knowledge of atmospheric status. The latter can be accurately characterized from radiosoundings, but these are usually unavailable. It can also be derived from meteorological model simulations, but spatial and temporal resolution are often too coarse. This study proposes performing atmospheric corrections by using temperature and relative humidity profiles acquired in flight from onboard sensors during data collection. Such measurements are used to document the atmospheric radiative transfer model MATISSE. First results from an experimentation over a tropical cropped area show that corrections are made with a 1.46 °K accuracy

Ihog and Boi are essential for Hedgehog signaling in Drosophila

<p>Abstract</p> <p>Background</p> <p>The Hedgehog (Hh) signaling pathway is important for the development of a variety of tissues in both vertebrates and invertebrates. For example, in developing nervous systems Hh signaling is required for the normal differentiation of neural progenitors into mature neurons. The molecular signaling mechanism underlying the function of Hh is not fully understood. In <it>Drosophila</it>, Ihog (Interference hedgehog) and Boi (Brother of Ihog) are related transmembrane proteins of the immunoglobulin superfamily (IgSF) with orthologs in vertebrates. Members of this IgSF subfamily have been shown to bind Hh and promote pathway activation but their exact role in the Hh signaling pathway has remained elusive. To better understand this role <it>in vivo</it>, we generated loss-of-function mutations of the <it>ihog </it>and <it>boi </it>genes, and investigated their effects in developing eye and wing imaginal discs.</p> <p>Results</p> <p>While mutation of either <it>ihog </it>or <it>boi </it>alone had no discernible effect on imaginal tissues, cells in the developing eye disc that were mutant for both <it>ihog </it>and <it>boi </it>failed to activate the Hh pathway, causing severe disruption of photoreceptor differentiation in the retina. In the anterior compartment of the developing wing disc, where different concentrations of the Hh morphogen elicit distinct cellular responses, cells mutant for both <it>ihog </it>and <it>boi </it>failed to activate responses at either high or low thresholds of Hh signaling. They also lost their affinity for neighboring cells and aberrantly sorted out from the anterior compartment of the wing disc into posterior territory. We found that <it>ihog </it>and <it>boi </it>are required for the accumulation of the essential Hh signaling mediator Smoothened (Smo) in Hh-responsive cells, providing evidence that Ihog and Boi act upstream of Smo in the Hh signaling pathway.</p> <p>Conclusions</p> <p>The consequences of <it>boi;ihog </it>mutations for eye development, neural differentiation and wing patterning phenocopy those of <it>smo </it>mutations and uncover an essential role for Ihog and Boi in the Hh signaling pathway.</p

États et sociétés en Europe centrale et orientale (XIXe-XXIe siècle)

Morgane Labbé, maîtresse de conférencesJérôme Heurtaux, maître de conférence à l’Université Paris-DauphineFrédéric Zalewski, maître de conférences à l’Université Paris-Ouest Nanterre La-Défense Enseignement suspendu durant l’année universitaire 2011-201

A new method for qualitative multi-scale analysis of bacterial biofilms on filamentous fungal colonies using confocal and electron microscopy

Bacterial biofilms frequently form on fungal surfaces and can be involved in numerous bacterial-fungal interaction processes, such as metabolic cooperation, competition, or predation. The study of biofilms is important in many biological fields, including environmental science, food production, and medicine. However, few studies have focused on such bacterial biofilms, partially due to the difficulty of investigating them. Most of the methods for qualitative and quantitative biofilm analyses described in the literature are only suitable for biofilms forming on abiotic surfaces or on homogeneous and thin biotic surfaces, such as a monolayer of epithelial cells.While laser scanning confocal microscopy (LSCM) is often used to analyze in situ and in vivo biofilms, this technology becomes very challenging when applied to bacterial biofilms on fungal hyphae, due to the thickness and the three dimensions of the hyphal networks. To overcome this shortcoming, we developed a protocol combining microscopy with a method to limit the accumulation of hyphal layers in fungal colonies. Using this method, we were able to investigate the development of bacterial biofilms on fungal hyphae at multiple scales using both LSCM and scanning electron microscopy (SEM). This report describes the protocol, including microorganism cultures, bacterial biofilm formation conditions, biofilm staining, and LSCM and SEM visualizations

Product line optimization with multiples sites

We consider the problem faced by a retailer that selects the set of products to allocate in finite capacity stores to maximize patronage. The purchase decision is made by customers that purchase exactly one product that maximizes her utility that depends on the product price, distance traveled to the store and reservation price, known to the retailer. The retailer's bilevel optimization problem is transformed into an integer optimization formulation. Small size instances are solved optimally, while for large instances, we explore Benders Decomposition, Branch and Cut and Cut and Branch to solve the problem. Our computational results show that the proposed Cut and Branch method obtains the best results, and improves on the current state of the art

Molecular Signatures of Sexual Communication in the Phlebotomine Sand Flies

Phlebotomine sand flies employ an elaborate system of pheromone communication wherein males produce pheromones that attract other males to leks (thus acting as an aggregation pheromone) and females to the lekking males (sex pheromone). In addition, the type of pheromone produced varies among populations. Despite the numerous studies on sand fly chemical communication, little is known of their chemosensory genome. Chemoreceptors interact with chemicals in an organism’s environment to elicit essential behaviors such as the identification of suitable mates and food sources. Thus, they play important roles during adaptation and speciation. Major chemoreceptor gene families, odorant receptors (ORs), gustatory receptors (GRs) and ionotropic receptors (IRs) together detect and discriminate the chemical landscape. Here, we annotated the chemoreceptor repertoire in the genomes of Lutzomyia longipalpis and Phlebotomus papatasi, major phlebotomine vectors in the New World and Old World, respectively. Comparison with other sequenced Diptera revealed a large and unique expansion where over 80% of the ~140 ORs belong to a single, taxonomically restricted clade. We next conducted a comprehensive analysis of the chemoreceptors in 63 L. longipalpis individuals from four different locations in Brazil representing allopatric and sympatric populations and three sex-aggregation pheromone types (chemotypes). Population structure based on single nucleotide polymorphisms (SNPs) and gene copy number in the chemoreceptors corresponded with their putative chemotypes, and corroborate previous studies that identified multiple populations. Our work provides genomic insights into the underlying behavioral evolution of sexual communication in the L. longipalpis species complex in Brazil, and highlights the importance of accounting for the ongoing speciation in central and South American Lutzomyia that could have important implications for vectorial capacity

Cartographier en 3D avec MapIt : une plus-value pour un parcours de professionnalisation selon la perspective étudiante

Implication Statement During the pandemic, the MapIt app was integrated into an occupational therapy program to support remote learning of built environment adaptation. MapIt maps rooms in a home, then generates a 3D model for viewing and taking virtual measurements. The students express that the use of MapIt during their training leads to embodying the roles expected of an occupational therapist. To inspire other good pedagogical ideas, this article presents how MapIt can support learning in authentic situations, a key element of a professionalization path, approaching the realities experienced by patients, clients, or caregivers.Énoncé des implications de la recherche Durant la pandémie, l’application MapIt a été intégrée dans un programme d’ergothérapie pour soutenir l’apprentissage à distance de l’adaptation de l’environnement bâti. MapIt permet de cartographier des pièces d’un domicile, puis d’en générer un modèle en 3D pour la visualisation et la prise de mesures virtuelles. Les étudiantes expriment que le recours à MapIt durant leur formation mène à incarner les rôles attendus d’une ergothérapeute. Pour inspirer d’autres bonnes idées pédagogiques, cet article présente comment MapIt peut soutenir l’apprentissage en situations authentiques, un élément clé d’un parcours de professionnalisation, s’approchant des réalités vécues par les personnes patientes, clientes ou intervenante

Group design in group testing for COVID-19 : A French case-study

Group testing is a screening strategy that involves dividing a population into several disjointed groups of subjects. In its simplest implementation, each group is tested with a single test in the first phase, while in the second phase only subjects in positive groups, if any, need to be tested again individually. In this paper, we address the problem of group testing design, which aims to determine a partition into groups of a finite population in such a way that cardinality constraints on the size of each group and a constraint on the expected total number of tests are satisfied while minimizing a linear combination of the expected number of false negative and false positive classifications. First, we show that the properties and model introduced by Aprahmian et al. can be extended to the group test design problem, which is then modeled as a constrained shortest path problem on a specific graph. We design and implement an ad hoc algorithm to solve this problem. On instances based on Santé Publique France data on Covid-19 screening tests, the results of the computational experiments are very promising

The Hepatokine TSK does not affect brown fat thermogenic capacity, body weight gain, and glucose homeostasis

Objectives Hepatokines are proteins secreted by the liver that impact the functions of the liver and various tissues through autocrine, paracrine, and endocrine signaling. Recently, Tsukushi (TSK) was identified as a new hepatokine that is induced by obesity and cold exposure. It was proposed that TSK controls sympathetic innervation and thermogenesis in brown adipose tissue (BAT) and that loss of TSK protects against diet-induced obesity and improves glucose homeostasis. Here we report the impact of deleting and/or overexpressing TSK on BAT thermogenic capacity, body weight regulation, and glucose homeostasis. Methods We measured the expression of thermogenic genes and markers of BAT innervation and activation in TSK-null and TSK-overexpressing mice. Body weight, body temperature, and parameters of glucose homeostasis were also assessed in the context of TSK loss and overexpression. Results The loss of TSK did not affect the thermogenic activation of BAT. We found that TSK-null mice were not protected against the development of obesity and did not show improvement in glucose tolerance. The overexpression of TSK also failed to modulate thermogenesis, body weight gain, and glucose homeostasis in mice

Impact of three years of large scale Indoor Residual Spraying (IRS) and Insecticide Treated Nets (ITNs) interventions on insecticide resistance in Anopheles gambiae s.l. in Benin

<p>Abstract</p> <p>Background</p> <p>In Benin, Indoor Residual Spraying (IRS) and long-lasting insecticidal nets (LLINs) are the cornerstones of malaria prevention. In the context of high resistance of <it>Anopheles gambiae </it>to pyrethroids, The National Malaria Control Program (NMCP) has undertaken a full coverage of IRS in a no-flood zone in the Oueme region, coupled with the distribution of LLINs in a flood zone. We assessed the impact of this campaign on phenotypic resistance, <it>kdr </it>(knock-down resistance) and <it>ace-1^R</it>(insensitive acetylcholinesterase) mutations.</p> <p>Methods</p> <p>Insecticides used for malaria vector control interventions were bendiocarb WP (0.4 g/m²) and deltamethrin (55 mg/m²), respectively for IRS and LLINs. Susceptibility status of <it>An. gambiae </it>was assessed using World Health Organization bioassay tests to DDT, permethrin, deltamethrin and bendiocarb in the Oueme region before intervention (2007) and after interventions in 2008 and 2010. <it>An. gambiae </it>specimens were screened for identification of species, molecular M and S forms and for the detection of the West African <it>kdr </it>(L1014F) as well as <it>ace-1^R</it>mutations using PCR techniques.</p> <p>Results</p> <p>The univariate logistic regression performed showed that <it>kdr </it>frequency has increased significantly during the three years in the intervention area and in the control area. Several factors (LLINs, IRS, mosquito coils, aerosols, use of pesticides for crop protection) could explain the selection of individual resistant <it>An. gambiae</it>. The <it>Kdr </it>resistance gene could not be the only mechanism of resistance observed in the Oueme region. The high susceptibility to bendiocarb is in agreement with a previous study conducted in Benin. However, the occurrence of <it>ace-1^R</it>heterozygous individuals even on sites far from IRS areas, suggests other factors may contribute to the selection of resistance other than those exerted by the vector control program.</p> <p>Conclusion</p> <p>The results of this study have confirmed that <it>An.gambiae </it>have maintained and developed the resistance to pyrethroids, but are still susceptible to bendiocarb. Our data clearly shows that selection of resistant individuals was caused by other insecticides than those used by the IRS and LLINs.</p