A life in progress: motion and emotion in the autobiography of Robert M. La Follette

This article is a study of a La Follette’s Autobiography, the autobiography of the leading Wisconsin progressive Robert M. La Follette, which was published serially in 1911 and, in book form, in 1913. Rather than focusing, as have other historians, on which parts of La Follette’s account are accurate and can therefore be trusted, it explains instead why and how this major autobiography was conceived and written. The article shows that the autobiography was the product of a sustained, complex, and often fraught series of collaborations among La Follette’s family, friends, and political allies, and in the process illuminates the importance of affective ties as well as political ambition and commitment in bringing the project to fruition. In the world of progressive reform, it argues, personal and political experiences were inseparable

The N-Terminal Domain of the Drosophila Retinoblastoma Protein Rbf1 Interacts with ORC and Associates with Chromatin in an E2F Independent Manner

The retinoblastoma (Rb) tumor suppressor protein can function as a DNA replication inhibitor as well as a transcription factor. Regulation of DNA replication may occur through interaction of Rb with the origin recognition complex (ORC).We characterized the interaction of Drosophila Rb, Rbf1, with ORC. Using expression of proteins in Drosophila S2 cells, we found that an N-terminal Rbf1 fragment (amino acids 1-345) is sufficient for Rbf1 association with ORC but does not bind to dE2F1. We also found that the C-terminal half of Rbf1 (amino acids 345-845) interacts with ORC. We observed that the amino-terminal domain of Rbf1 localizes to chromatin in vivo and associates with chromosomal regions implicated in replication initiation, including colocalization with Orc2 and acetylated histone H4.Our results suggest that Rbf1 can associate with ORC and chromatin through domains independent of the E2F binding site. We infer that Rbf1 may play a role in regulating replication directly through its association with ORC and/or chromatin factors other than E2F. Our data suggest an important role for retinoblastoma family proteins in cell proliferation and tumor suppression through interaction with the replication initiation machinery

Multicriteria analysis on rock moisture and streamflow in a rainfall-runoff model improves accuracy of model results

Although shallow ((Formula presented.) 1.5 m) soil water storage has been extensively studied, the significance of deeper unsaturated zone water storage to flow generation is poorly documented. However, a limited but growing body of empirical work shows that the weathered bedrock vadose zone, not soil, stores the majority of plant available water in many seasonally dry and semi-arid landscapes. Moreover, this storage dynamic mediates recharge to hillslope groundwater systems that generate stream discharge and support ecologically significant baseflows. Explicit representations of bedrock vadose zone processes are rarely incorporated into runoff models, due in part to a paucity of observations that can constrain simulations. Here, we develop a simple representation of the weathered bedrock vadose zone that is guided by in situ field observations. We incorporate this representation into a rainfall-runoff model, and calibrate it on streamflow alone, on rock moisture (i.e., weathered bedrock vadose zone moisture) alone, and on both using the concept of Pareto optimality. We find that the model is capable of accurately simultaneously simulating dynamics in rock moisture and streamflow, in terms of Kling-Gupta Efficiency, when using Pareto optimal parameter sets. Calibration on streamflow alone, however, is insufficient to accurately simulate rock moisture dynamics. We further find that the posterior distributions of some model parameters are sensitive to choice of calibration scenario. The posterior distribution of high-performing model parameters resulting from the streamflow only calibration scenario include physically unrealistic values that are not yielded by the rock moisture only or Pareto calibration strategies. These results suggest that the accuracy of some model results can be increased and parameter uncertainty decreased via incorporation of rock moisture data in calibration, without sacrificing streamflow simulation quality. Emerging recognition of the global significance of weathered bedrock water storage in seasonally dry and semi-arid regions motivates more observations of weathered bedrock moisture and integration of this variable into earth system models