Avaliação do efeito antimicrobiano in vitro dos extratos etanólicos de Plectranthus neochilus e Cnidoscolus quercifolius

Avaliou-se o efeito antimicrobiano in vitro dos extratos etanólicos de Plectranthus neochilus e Cnidoscolus quercifolius de forma isolada e associadas. Na 1ª etapa, utilizaram-se os extratos em concentrações 1%, 2,5%, 5% e 10%. Na 2ª etapa, utilizaram-se apenas extratos brutos. Para o teste de sensibilidade dos extratos, utilizaram-se cepas padrão Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853. Nas duas etapas, realizaram-se os mesmos testes de sensibilidade. As placas foram semeadas e lidas após 24 horas de incubação em estufa a 37ºC. De acordo com os resultados, conclui-se que não houve atividade bacteriana dos extratos etanólicos estudados isolados, bem como associados diante das cepas padrão de Staphylococcus aureus, Escherichia coli e Pseudomonas aeruginosa nas diferentes metodologias aplicadas quando comparadas com o antibiótico padrão (amicacina)

EVALUATION OF THE EFFECTS OF OZONE THERAPY AND CISPLATIN IN AN EXPERIMENTAL MODEL IN MICE WITH EHRLICH CARCINOMA

The present objective was to evaluate Swiss albino mice (Mus muscullus) with Ehrlich carcinoma treated with intrarectal ozone therapy and cisplatin. Twenty-four female mice, approximately 60 days of age, varying between 35g and 40g in weight were divided into four groups, Group G1: Positive control: 2.5mg/kg of cisplatin; Group G2: Ozone-oxygen mix via rectal insufflation (MOO IR); Group G3: 2.5mg/kg of cisplatin + (MOO IR), and Group G4: Negative control with 1ml of saline 0.9%. All animals underwent an eight-day adaptation period. Forty-eight hours after inoculation, the treatments were performed for six days. The mice were euthanized at the end of the experiment and the tumor was removed. There were statistically significant differences between groups for tumor weight. Mean tumor weight was greater in group G4, and lesser in group G3. These significant differences were observed between the negative control and other groups. On histopathology, there were no significant differences between groups. It is concluded that combined ozone therapy and cisplatin was shown to be the treatment where mice with Ehrlich carcinoma had the least tumor weight

Evaluation of the effects of ozone therapy and cisplatin in an experimental model in mice with Ehrlich carcinoma

The present objective was to evaluate Swiss albino mice (Mus muscullus) with Ehrlich carcinoma treated with intrarectal ozone therapy and cisplatin. Twenty-four female mice, approximately 60 days of age, varying between 35g and 40g in weight were divided into four groups, Group G1: Positive control: 2.5mg/kg of cisplatin; Group G2: Ozone-oxygen mix via rectal insufflation (MOO IR); Group G3: 2.5mg/kg of cisplatin + (MOO IR), and Group G4: Negative control with 1ml of saline 0.9%. All animals underwent an eight-day adaptation period. Forty-eight hours after inoculation, the treatments were performed for six days. The mice were euthanized at the end of the experiment and the tumor was removed. There were statistically significant differences between groups for tumor weight. Mean tumor weight was greater in group G4, and lesser in group G3. These significant differences were observed between the negative control and other groups. On histopathology, there were no significant differences between groups. It is concluded that combined ozone therapy and cisplatin was shown to be the treatment where mice with Ehrlich carcinoma had the least tumor weight.Ehrlich carcinoma is an aggressive, rapidly-growing tumor used as an experimental model in female mice because it corresponds to mammary adenocarcinoma. The present objective was to evaluate Swiss albino mice (Mus musculus) with Ehrlich carcinoma treated with intrarectal ozone therapy and cisplatin. Twenty-four female mice, approximately 60 days of age, varying between 35g and 43g in weight were divided into four groups, Group 1 (G1): 1mL of cisplatin 2.5mg/kg, orally; Group 2 (G2): Ozone-oxygen mixture (OOM) 20 µg/mL, via rectal insufflation (RI); Group 3 (G3): 1mL of cisplatin 2.5mg/kg, orally+ 20 µg/mL (OOM RI), and Group 4 (G4): control with 1mL of saline 0.9%, orally. All animals underwent an eight-day adaptation period. Forty-eight hours after inoculation, the treatments were performed daily, for six days. The mice were euthanized at the end of the experiment, after treatment, and the tumor was removed. There were statistically significant differences between groups for tumor weight. Mean tumor weight was greater in G4 (3,83 ± 1.20), and lesser in G3 (0.79 ± 0.73). These significant differences were observed between the group G4 and other groups. On histopathology, there were no significant differences between groups. It is concluded that ozone therapy associated with cisplatin proved to be the treatment in which mice with Erhlich's carcinoma showed delay in tumor growth, therefore, the lowest tumor weight

Avaliação do efeito da ozonioterapia e cisplatina em modelo experimental de camundongos com carcinoma de Ehrlich.

Neoplasias representam uma das principais causas de morte em humanos e animais, é uma doença complexa caracterizada pela modificação permanente do material genético das células. A ozonioterapia é descrita como uma abordagem terapêutica para o tratamento coadjuvante do câncer e dos efeitos colaterais associados à quimioterapia e radioterapia. O objetivo deste trabalho foisemp avaliar os camundongos albinos Swiss (Mus muscullus) com carcinoma de Ehrlich submetidos aos tratamentos com ozonioterapia intrarretal e cisplatina. O experimento, consistiu na utilização de 25 camundongos, albinos Swiss (Mus muscullus), fêmeas com aproximadamente 60 dias de idade, apresentando peso corpóreo entre 35g e 40g, inoculados com o carcinoma de Ehrlich, divididos em quatro grupos, Grupo G1: Controle positivo: 2,5mg/kg de Cisplatina; Grupo G2: Mistura Ozônio Oxigênio via Insuflação Retal; Grupo G3: 2,5mg/kg de Cisplatina + Mistura Ozônio Oxigênio via Insuflação Retal e Grupo G4: Controle negativo 1ml de soro Fisiológico 0,9%. Os animais de todos os grupos foram submetidos a um período de adaptação de oito dias, em que nos grupos G2 e G3 foram submetidos a ozonioterapia via insuflação retal durante seis dias. Após este período realizou-se a inoculação das células do carcinoma de Ehrlich em todos os animais. Após 48h da inoculação se iniciou os diferentes tratamentos durante seis dias, ao fim do experimento, foi realizada a eutanásia dos animais e feita a coleta de sangue, através da punção cardíaca, e a remoção do tumor e órgãos (pulmão, baço, rins e fígado). Os resultados relacionados com o hemograma, perfil renal e hepático não apresentaram diferenças estatisticamente significativas (p < 0,05) entre os grupos para as variáveis analisadas. No peso do tumor e de cada um dos órgãos analisados (Pulmão, Baço, Rim, Fígado) apresentaram diferenças estatisticamente significativas entre os grupos. Para estas variáveis se destaca que a média do peso do tumor foi maior no grupo G4 (3,83 g), foi menor no grupo G3 (0,79 g). E essas diferenças significativas foram notadas entre o controle negativo com os demais grupos. No exame histopatológico não foram notadas diferenças estatisticamente significativas (p < 0,05) entre grupos Conclui-se que de acordo com os resultados a ozonioterapia associada a cisplatina demonstrou ser o tratamento em que os em camundongos com carcinoma de Erhlich apresentaram o menor peso do tumor. As ações terapêuticas no grupo tratado apenas com ozonioterapia proporcionaram aumento no consumo do alimento, como também, uma atividade antitumoral semelhante ao grupo tratado apenas com cisplatina.Neoplasms represent one of the main causes of death in humans and animals, it is a complex disease characterized by permanent modification of the cell's genetic material. Ozone therapy is described as a therapeutic approach for the coadjutant treatment of cancer and side effects associated with chemotherapy and radiotherapy. This paper aims to evaluate albino Swiss mice (Mus muscullus) with Ehrlich carcinoma submitted to treatments with intrarectal ozone therapy and cisplatin. The experiment consisted of using 25 Swiss albino mice (Mus muscullus), females with approximately 60 days old, with body weight between 35g and 40g, inoculated with Ehrlich carcinoma, divided into four groups, Group G1: Positive control: 2.5mg / kg of Cisplatin; Group G2: Ozone-Oxygen Mixture via Rectal Insufflation; Group G3: 2.5mg / kg of Cisplatin + Ozone-Oxygen Mixture via Rectal Insufflation and Group G4: Negative control 1ml of 0.9% saline solution. Animals in all groups underwent an adaptation period of eight days, in which groups G2 and G3 were subjected to ozone therapy via rectal insufflation for six days. After this period, Ehrlich carcinoma cells were inoculated in all animals. After 48h of the inoculation, the different treatments were started for six days, at the end of the experiment the animals were euthanized, blood was collected through cardiac puncture, and the tumor and organs were removed (lung, spleen, kidneys and liver). The results related to the blood count, renal and hepatic profile did not show statistically significant differences (p <0.05) between the groups for the analyzed variables. The weight of the tumor and each of the organs analyzed (Lung, Spleen, Kidney, Liver) showed statistically significant differences between the groups. For these variables, it is highlighted that the tumor average weight was higher in the G4 group (3.83 g), it was lower in the G3 group (0.79 g). And these significant differences were noted between the negative control group with the other groups. In the histopathological exam, there were no statistically significant differences (p <0.05) between groups. It is concluded that, according to the results, ozone therapy associated with cisplatin proved to be the treatment in which mice with Erhlich carcinoma had the lighter tumor weight. The therapeutic actions in the group treated only with ozone therapy provided an increase in food consumption, as well as an antitumor activity similar to the group treated only with cisplatin.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNP