97 research outputs found

    大時代的文化研究 : 馬照跑,舞照跳? = Will horse racing and dancing go on in the Great Era of Cultural Studies?

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    研討會分為二部分。第一部分的主題是「大時代的文化研究:馬照跑,舞照跳?」,以MCSian的論文為引旨,透過對話去思考在當前處境如何尋找出路,由鄧芝珊主持,分別由廖可兒主講〈從聯合國氣候變化大會到黃背心運動–反思「環境 vs 發展與生計」的二元對立〉,馮羚主講〈從台灣電音三太子看傳統文化的傳承與挑戰〉,譚家浚主講〈賽馬作為統治殖民手段 - 由被統治者的接受與反抗所演變成的香港賽馬文化〉,並由李小良評論及與講者進行討論

    此時彼刻文化研究 = Cultural studies still in the making

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    時光荏苒,MCS15年了! 今年MCS年度研討會與過去有點不同,主題是「展演CROSSOVER:香港文化研究的變奏與另類新選擇」。在舉步維艱的社會政治泥沼中,我們如何理解和面對這境地?透過這次研討會,MCS表達對這個時代的關切,並在這關口中提出新概念,加入「表演研究」元素──不但帶來學術上的協同效應,而且使未來教育的路更闊、與大家走得更遠,發揮更大的潛質。 研討會分為兩部分。第一部分以MCSian的論文為引旨,透過對話去思考在當前處境如何尋找出路,由梁旭明主持,分別由吳紹奇主講〈作為歷史哲學家的班雅明:論歷史哲學兼評〈歷史哲學論綱〉〉,Kong Yee主講〈The Identity of Cheung Chau Kai-fong : The Cultural Disparity of The Northern and Southern Cheung Chau〉,古卓嵐主講〈Modern Educayshun 的啟示──從恐懼女性主義心理 到課室政治的省思〉,並由羅冠聰評論及與講者進行討論

    Robust estimation of bacterial cell count from optical density

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    Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data
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