7 research outputs found
Avaliação da segurança e ocorrência de defeitos na operação de corte semimecanizado de florestas de eucalipto
Avaliação qualitativa de impactos ambientais considerando as etapas de limpeza e preparo do terreno em plantios florestais no Nordeste paraense
The invertebrate's community in adjacent Alto Iguaçu's anthropic lakes of different environmental factors
RNA interference inhibits yellow fever virus replication in vitro and in vivo
RNA interference (RNAi) is a process that is induced by double stranded RNA and involves the degradation of specific sequences of mRNA in the cytoplasm of the eukaryotic cells. It has been used as an antiviral tool against many viruses, including flaviviruses. The genus Flavivirus contains the most important arboviruses in the world, i.e., dengue (DENV) and yellow fever (YFV). In our study, we investigated the in vitro and in vivo effect of RNAi against YFV. Using stable cell lines that expressed RNAi against YFV, the cell lines were able to inhibit as much as 97% of the viral replication. Two constructions (one against NS1 and the other against E region of YFV genome) were able to protect the adult Balb/c mice against YFV challenge. The histopathologic analysis demonstrated an important protection of the central nervous system by RNAi after 10 days of viral challenge. Our data suggests that RNAi is a potential viable therapeutic weapon against yellow fever.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP