DNA multigene characterization of Fasciola hepatica and Lymnaea neotropica and its fascioliasis transmission capacity in Uruguay, with historical correlation, human report review and infection risk analysis

Fascioliasis is a highly pathogenic zoonotic disease emerging in recent decades, in part due to the effects of climate and global changes. South America is the continent presenting more numerous human fascioliasis endemic areas and the highest Fasciola hepatica infection prevalences and intensities known in humans. These serious public health scenarios appear mainly linked to altitude areas in Andean countries, whereas lowland areas of non-Andean countries, such as Uruguay, only show sporadic human cases or outbreaks. To understand this difference, we characterized F. hepatica from cattle and horses and lymnaeids of Uruguay by sequencing of ribosomal DNA ITS-2 and ITS-1 spacers and mitochondrial DNA cox1, nad1 and 16S genes. Results indicate that vectors belong to Lymnaea neotropica instead of to Lymnaea viator, as always reported from Uruguay. Our correlation of fasciolid and lymnaeid haplotypes with historical data on the introduction and spread of livestock species into Uruguay allow to understand the molecular diversity detected. We study the life cycle and transmission features of F. hepatica by L. neotropica of Uruguay under standardized experimental conditions to enable a comparison with the transmission capacity of F. hepatica by Galba truncatula at very high altitude in Bolivia. Results demonstrate that although L. neotropica is a highly efficient vector in the lowlands, its transmission capacity is markedly lower than that of G. truncatula in the highlands. On this baseline, we review the human fascioliasis cases reported in Uruguay and analyze the present and future risk of human infection in front of future climate change estimations

Violência sexual contra adolescentes: notificações nos conselhos tutelares, Campo Grande, Mato Grosso do Sul, Brasil La violencia sexual contra adolescentes: notificaciones en los consejos tutelares, Campo Grande, Mato Grosso do Sul, Brasil Sexual violence against adolescents: notifications filed in the child and adolescent protective services in the city of Campo Grande, state of Mato Grosso do Sul, Brazil

Estudo com o objetivo de verificar nos Conselhos Tutelares Norte e Sul do município de Campo Grande, Mato Grosso do Sul, a ocorrência de notificações de violência sexual contra adolescentes em 2007 e 2008. Pesquisa descritiva quantitativa retrospectiva baseada em dados dos arquivos de notificações de violência sexual contra adolescentes. A maioria das vítimas era do sexo feminino. Houve prevalência da faixa etária de 10 a 14 anos, para ambos os sexos. Predominaram agressores masculinos. A maior parte dos atos de violência sexual notificados ocorreu em ambiente doméstico. A abordagem do profissional de saúde frente aos casos de violência sexual deve ser permeada por respeito para com a vítima e sua família. Para tanto, requer-se um atendimento acolhedor, que assegure a privacidade necessária para que o adolescente possa expressar os fatos e manifestar seus sentimentos.<br>Estudio desarrollado con el objetivo de verificar la ocurrencia de notificaciones de violencia sexual contra adolescentes en 2007 y 2008 en los Consejos Tutelares Norte y Sur de Campo Grande, Mato Grosso do Sul, Brasil. Investigación descriptiva cuantitativa retrospectiva con base en archivos de notificaciones de violencia sexual contra adolescentes. La mayoría de las víctimas era de sexo femenino. Se registró una prevalencia de grupo etario de 10 a 14 años para adolescentes del sexo femenino y del masculino. Predominaron agresores masculinos. La mayor parte de los actos de violencia sexual verificados ocurrió en el ambiente del hogar. El abordaje del profesional de la salud frente a los casos de violencia sexual debe estar imbuido de respeto para con la víctima y su familia. A tal fin, se hace necesaria una atención acogedora, que asegure la privacidad necesaria como para que el adolescente pueda expresarse acerca de lo sucedido y manifestar sus sentimientos.<br>This study examines reports of sexual violence against adolescents in 2007 and 2008 made with the North- and South-District Child and Adolescent Protective Services of Campo Grande, the capital city of the state of Mato Grosso do Sul, Brazil. Data for this retrospective, descriptive and quantitative research were collected from the services' archives on sexual violence against adolescents. Results: most victims were females. Reports predominantly concerned boys and girls from 10 to 14 years of age. Most offenders were males. The majority of reported cases occurred at home. When addressing cases of sexual violence, healthcare professionals should adopt a stance permeated by respect for the victims and their families. This requires providing a receptive environment, so as to ensure the level of privacy required for victimized adolescents to report facts and express their feelings