27 research outputs found
SMAD-4 altered nucleo-cytoplasmic shuttling in thyroid tumours: a new intriguing link in cancer development
No full textIncreased TbRII expression reduces tumorigenicity and induces neuronal differentiation of human neuroblastoma cells
No full text
Anatomia Patologica e basi molecolari dei carcinomi differenziati tiroidei (Capitolo 5)
No full textVolume n°25 della Collana Monografica della Società Italiana di Chirurgia, V.le Tiziano, 19-Roma
Transforming growth factor-beta pathway in human renal cell carcinoma and surrounding normal-appearing renal parenchyma.
No full textOBJECTIVE:
To analyze the role of the transforming growth factor (TGF)-beta pathway in renal tumors and to verify whether alterations in TGF-beta 1 pathway expression are associated with the grade of tumor differentiation and pathologic stage in renal cell carcinomas.
STUDY DESIGN:
The expression of TGF-beta 1 and TGF-beta receptors (T beta RI and T beta RII), SMAD-2 and SMAD-4 was investigated by immunohistochemistry in normal peritumoral and tumoral tissue from 53 renal cell carcinomas (clear cell type). The gene expression of SMAD-2 and SMAD-4 was also studied by reverse transcription polymerase chain reaction (RT-PCR) in normal peritumoral and tumoral tissue from 6 of 56 primary tumors.
RESULTS:
TGF-beta 1, T beta RI and T beta RII immunoreactivity was more frequent in tumoral than in normal peritumoral renal tissue (96.22%, 79.25% and 75.41% vs. 88.37%, 69.76% and 62.69%), whereas SMAD-2 and SMAD-4 immunoreactivity was more frequent in normal peritumoral than in tumoral tissue (23.25% and 30.23% vs. 15.09% and 7.54%). In tumor areas, immunohistochemical scores were lower for T beta RII than for T beta RI and TGF-beta 1 and higher than SMAD-4 and SMAD-2 scores. TGF-beta 1, T beta RI, T beta RII and SMAD-4 histologic scores correlated with neither the histologic grade of malignancy nor TNM clinical stage, whereas SMAD-2 protein levels were significantly lower in grade 3 than in grade 1 tumors. In the samples of normal kidney and carcinoma studied, RT-PCR detected the correct transcripts for SMAD-2 and SMAD-4, indicating that the RNA of the samples analyzed contained RNA sequences coding for these genes.
CONCLUSION:
Our data support the concept that the reduction of T beta RII and SMAD proteins in renal cell carcinomas is involved in tumor development and suggest an altered TGF-beta/SMAD signaling pathway in kidney neoplasia
Loss of TGFb mediated growth regulation in transformed thyroid cells is associated with alterations in the expression of TGFb receptors.
No full textEffetti sulla proliferazione e sulle funzioni di cellule di tiroide normali e TGFb resistenti
No full textpubblicata in Tumor
THE PHOSPHATASE INHIBITOR OKADAIC ACID STIMULATED THE TSH-INDUCED G1-S PHASE TRANSITION IN THYROID CELLS
No full textLoss of TGFb mediated growth regulation in K-Ras transformed thyroid cells is asssociated with reduced expression of Type II TGFb Receptor.
No full text
