Simulated filtration pond to remove Escherichia coli from irrigation water.

The United States Food and Drug Administration?s (FDA) proposed water rules to implement the Food Safety Modernization Act (FSMA) could leave some growers, especially those who rely on an irrigation system based on recycled water, unable to irrigate fresh produce with their irrigation water, especially those who rely on an irrigation system based on recycled water. Irrigation water could be treated with chlorine, ozone, or other product to reduce the bacterial load in the water; however, at present not one of these options has been approved by the Environmental Protection Agency for treating irrigation water. In an attempt to reduce the number of bacteria present in irrigation water entering a farm, a simulated filter pond was constructed using gravel, sand, and silt-loam soil. The filter pond sought to utilize in part what occurs naturally with the filtration of water through the soil profile. This natural process provides clean water in wells and aquifers. The simulated pond reduced the Escherichia coli load in water by 95% with a flow rate of 3.9 gal/h/yd2. In order to increase the water productivity of the simulated filter pond, most of the dirt was removed; subsequently the E. coli filtration rate went to 55% and 46%, with flow rates of 12.9 gal/h/yd2 and 17.6 gal/h/yd2, respectively

Movement of Escherichia coli in soil as applied in irrigation water.

The US Food and Drug Administration (FDA) has proposed that If irrigation water exceeds 235 colony-forming units (CFU) of E. coli /100 ml in any one sample or 126 CFU/100 ml in the average of any five consecutive samples, growers would have to cease using that water in any way that directly contacts the surface of fresh produce (FDA 2013). The FDA has proposed that these E. coli levels are an indication of high risk of bacterial contamination of fresh onion (Allium cepa L.) bulbs regardless of the irrigation system. If onion irrigation exceeds 235 CFU, it is not known whether the contaminated water applied by furrow or drip irrigation actually reaches the onion bulb. Soil could filter E. coli and other bacteria before irrigation water reaches onion bulbs. ?Vaquero? onions were grown on Owyhee silt loam. In our preliminary studies reported here, well water free of E. coli was applied to onions through drip irrigation or through furrow irrigation. A second water source was intentionally enriched with E. coli by being run across a pasture and recaptured prior to use. Furrow and drip irrigation were used to apply this water containing 218 to >2400 MPN/100ml for 11+ hours per irrigation. E. coli was monitored in the soil water at the end of irrigation cycles through direct sampling of the soil. Soil water was also sampled using sterile soil solution capsules (SSSC) to sample E. coli in the soil water that moved into place, to differentiate the movement of soil water from the soil water already in place. Soil water measurements were made adjacent to the water source, half way to the bulbs, and immediately adjacent to the onion bulbs. For furrow irrigation with ditch water the E. coli counts in the soil next to the onion bulbs was only 0% and 21% of the counts in the irrigation water following the first and second irrigations, respectively. During subsequent furrow irrigations, the E. coli counts in the soil water next to the onion bulbs exceeded the counts in the irrigation water. For drip irrigation with ditch water, the E. coli counts in the soil solution next to the onion bulbs remained very low. The soil water sampled by the SSSC adjacent to the onion bulbs drip-irrigated with ditch water also had very low E. coli counts

Survival of Escherichia coli on onion during field curing and packout.

The Food and Drug administration has expressed concern that Onions (Allium cepa) irrigated with water contaminated with high rates of Escherichia coli could harbor E. coli on their surface or interior. On the other hand, since onions contain antimicrobial compounds and field conditions may not be conducive to E. coli survival, the E. coli population on the surface of onions might become negligible through the course of field curing. Further, the relationship between the E. coli in the irrigation water to the E. coli on onion bulbs after field curing, harvest, and packout has not been studied. To determine if E. coli should be of concern in onion production, we sought to measure the die-off of E. coli on onions between the last irrigation and harvest and the presence of E. coli on onions after packout. Well water was tested and had no E. coli; ditch water intentionally run across a pasture prior to use had 218 to > 2400 MPN of E. coli/100ml. Onions were sampled from those furrow irrigated (ditch water) and those drip irrigated (well water) starting at lifting 3 September 2013 for four consecutive weeks. At 0 and 28 days after lifting, both interior and exterior of the onions were tested for E. coli. At 7, 14, and 21 days after lifting, only the exterior of the onions was tested. None of the onions contained E. coli internally at 0 or 28 days after lifting. At lifting E. coli was present on the exterior of both the drip and furrow irrigated onions and seemed to be largely unrelated to the irrigation water. The exterior E. coli contamination decreased rapidly after lifting. After harvest and packout on 14 October 2013, no E. coli was detected on the onion bulb exteriors from either irrigation treatment. E. coli introduced into the onion field through furrow irrigation was not present on or in the packed out onion bulbs

Photometric Redshifts of Quasars

We demonstrate that the design of the Sloan Digital Sky Survey (SDSS) filter system and the quality of the SDSS imaging data are sufficient for determining accurate and precise photometric redshifts (``photo-z''s) of quasars. Using a sample of 2625 quasars, we show that photo-z determination is even possible for z<=2.2 despite the lack of a strong continuum break that robust photo-z techniques normally require. We find that, using our empirical method on our sample of objects known to be quasars, approximately 70% of the photometric redshifts are correct to within delta z = 0.2; the fraction of correct photometric redshifts is even better for z>3. The accuracy of quasar photometric redshifts does not appear to be dependent upon magnitude to nearly 21st magnitude in i'. Careful calibration of the color-redshift relation to 21st magnitude may allow for the discovery of on the order of 10^6 quasars candidates in addition to the 10^5 quasars that the SDSS will confirm spectroscopically. We discuss the efficient selection of quasar candidates from imaging data for use with the photometric redshift technique and the potential scientific uses of a large sample of quasar candidates with photometric redshifts.Comment: 29 pages, 8 figures, submitted to A

Insights into the Molecular Basis of L-Form Formation and Survival in Escherichia coli

L-forms have been shown to occur among many species of bacteria and are suspected to be involved in persistent infections. Since their discovery in 1935, numerous studies characterizing L-form morphology, growth, and pathogenic potential have been conducted. However, the molecular mechanisms underlying the formation and survival of L-forms remain unknown. Using unstable L-form colonies of Escherichia coli as a model, we performed genome-wide transcriptome analysis and screened a deletion mutant library to study the molecular mechanisms involved in formation and survival of L-forms. Microarray analysis of L-form versus classical colonies revealed many up-regulated genes of unknown function as well as multiple over-expressed stress pathways shared in common with persister cells and biofilms. Mutant screens identified three groups of mutants which displayed varying degrees of defects in L-form colony formation. Group 1 mutants, which showed the strongest defect in L-form colony formation, belonged to pathways involved in cell envelope stress, DNA repair, iron homeostasis, outer membrane biogenesis, and drug efflux/ABC transporters. Four (Group 1) mutants, rcsB, a positive response regulator of colanic acid capsule synthesis, ruvA, a recombinational junction binding protein, fur, a ferric uptake regulator and smpA a small membrane lipoprotein were selected for complementation. Complementation of the mutants using a high-copy overexpression vector failed, while utilization of a low-copy inducible vector successfully restored L-form formation. This work represents the first systematic genetic evaluation of genes and pathways involved in the formation and survival of unstable L-form bacteria. Our findings provide new insights into the molecular mechanisms underlying L-form formation and survival and have implications for understanding the emergence of antibiotic resistance, bacterial persistence and latent infections and designing novel drugs and vaccines

Global Analysis of Extracytoplasmic Stress Signaling in Escherichia coli

The Bae, Cpx, Psp, Rcs, and σE pathways constitute the Escherichia coli signaling systems that detect and respond to alterations of the bacterial envelope. Contributions of these systems to stress response have previously been examined individually; however, the possible interconnections between these pathways are unknown. Here we investigate the dynamics between the five stress response pathways by determining the specificities of each system with respect to signal-inducing conditions, and monitoring global transcriptional changes in response to transient overexpression of each of the effectors. Our studies show that different extracytoplasmic stress conditions elicit a combined response of these pathways. Involvement of the five pathways in the various tested stress conditions is explained by our unexpected finding that transcriptional responses induced by the individual systems show little overlap. The extracytoplasmic stress signaling pathways in E. coli thus regulate mainly complementary functions whose discrete contributions are integrated to mount the full adaptive response

Application of protons to computer tomography

It was demonstrated that the application of protons to computed tomography can result in a significant dose advantage relative to x rays. Thus, at the same dose as is delivered by contemporary commercial x-ray scanners, a proton scanner could produce reconstructions with a factor of 2 or more improvement in density resolution. Whether such an improvement can result in significantly better diagnoses of human disease is an open question which can only be answered by the implementation of a proton scanner in a clinical situation