Ingraft chimerism in lung transplantation - a study in a porcine model of obliterative bronchiolitis

<p>Abstract</p> <p>Background</p> <p>Bronchial epithelium is a target of the alloimmune response in lung transplantation, and intact epithelium may protect allografts from rejection and obliterative bronchiolitis (OB). Herein we study the influence of chimerism on bronchial epithelium and OB development in pigs.</p> <p>Methods</p> <p>A total of 54 immunosuppressed and unimmunosuppressed bronchial allografts were serially obtained 2-90 days after transplantation. Histology (H&E) was assessed and the fluorescence in situ hybridization (FISH) method for Y chromosomes using pig-specific DNA-label was used to detect recipient derived cells in graft epithelium and bronchial wall, and donor cell migration to recipient organs. Ingraft chimerism was studied by using male recipients with female donors, whereas donor cell migration to recipient organs was studied using female recipients with male donors.</p> <p>Results</p> <p>Early appearance of recipient-derived cells in the airway epithelium appeared predictive of epithelial destruction (<it>R </it>= 0.610 - 0.671 and <it>p </it>< 0.05) and of obliteration of the bronchial lumen (<it>R </it>= 0.698 and <it>p </it>< 0.01). All allografts with preserved epithelium showed epithelial chimerism throughout the follow-up. Antirejection medication did not prevent, but delayed the appearance of Y chromosome positive cells in the epithelium (<it>p </it>< 0.05), or bronchial wall (<it>p </it>< 0.05).</p> <p>Conclusions</p> <p>In this study we demonstrate that early appearance of Y chromosomes in the airway epithelium predicts features characteristic of OB. Chimerism occurred in all allografts, including those without features of OB. Therefore we suggest that ingraft chimerism may be a mechanism involved in the repair of alloimmune-mediated tissue injury after transplantation.</p

MHC class I A region diversity and polymorphism in macaque species

The HLA-A locus represents a single copy gene that displays abundant allelic polymorphism in the human population, whereas, in contrast, a nonhuman primate species such as the rhesus macaque (Macaca mulatta) possesses multiple HLA-A-like (Mamu-A) genes, which parade varying degrees of polymorphism. The number and combination of transcribed Mamu-A genes present per chromosome display diversity in a population of Indian animals. At present, it is not clearly understood whether these different A region configurations are evolutionarily stable entities. To shed light on this issue, rhesus macaques from a Chinese population and a panel of cynomolgus monkeys (Macaca fascicularis) were screened for various A region-linked variations. Comparisons demonstrated that most A region configurations are old entities predating macaque speciation, whereas most allelic variation (>95%) is of more recent origin. The latter situation contrasts the observations of the major histocompatibility complex class II genes in rhesus and cynomolgus macaques, which share a high number of identical alleles (>30%) as defined by exon 2 sequencing

Crisia and Crisidia (Bryozoa Cyclostomata) from the upper Maastrichtian near Maastricht

The genera Crisia and Crisidia (family Crisiidae, Bryozoa Cyclostomata) are reported from the Upper Maastrichtian near Maastricht, eliminating all doubts as to the Cretaceous origin of the Crisiidae. In addition, the oldest-known Tertiary occurrences of the various crisiid genera are briefly discussed.Les genres Crisia et Crisidia (famille Crisiidae, Bryozoa Cyclostomata) sont rapportés pour la première fois comme provenant du Maastrichtien supérieur dans la région-type. Cette découverte donne une base plus ferme en ce qui concerne l'origine des Crisiidae dans le Crétacé. En outre les plus anciens représentants des genres des Crisiidae dans le Tertiaire sont brièvement discutés.Lagaaij Robert. Crisia and Crisidia (Bryozoa Cyclostomata) from the upper Maastrichtian near Maastricht. In: Documents des Laboratoires de Géologie de la Faculté des Sciences de Lyon. Hors série n°3, 1975. Bryozoa 1974 – Proceedings of the third Conference International Bryozoology Association – Lyon (fasc. 2) pp. 583-594

Letter to H.B. Stenzel from R. Lagaaij on 1963-08-14

Jackson School of Geoscience

Scientific publications by Robert Lagaaij

Lagaaij Robert. Scientific publications by Robert Lagaaij. In: Documents des Laboratoires de Géologie de la Faculté des Sciences de Lyon. Hors série n°3, 1975. Bryozoa 1974 – Proceedings of the third Conference International Bryozoology Association – Lyon (fasc. 1) p. 12

Letter to H.B. Stenzel from R. Lagaaij on 1963-07-06

Jackson School of Geoscience

Letter to H.B. Stenzel from Robert Lagaaij on 1959-02-08

Jackson School of Geoscience

Letter to H.B. Stenzel from Robert Lagaaij on 1961-08-05

Jackson School of Geoscience

Letter to Henryk B. Stenzel from R. Lagaaij on 1964-06-21

Jackson School of Geoscience

Letter to H.B. Stenzel from Robert Lagaaij on 1960-05-29

Jackson School of Geoscience