Polyanalytic Hardy decomposition of higher order Lipschitz functions

This paper is concerned with the problem of decomposing a higher order Lipschitz function on a closed Jordan curve $\Gamma$ into a sum of two polyanalytic functions in each open domain defined by $\Gamma$. Our basic tools are the Hardy projections related to a singular integral operator arising in polyanalytic function theory, which, as it is proved here, represents an involution operator on the higher order Lipschitz classes. Our result generalizes the classical Hardy decomposition of Holder continuous functions on the boundary of a domain

Decomposition of first order Lipschitz functions by Clifford algebra-valued harmonic functions

In this paper we solve the problem on finding a sectionally Clifford algebra-valued harmonic function, zero at infinity and satisfying certain boundary value condition related to higher order Lipschitz functions. Our main tool are the Hardy projections related to a singular integral operator arising in bimonogenic function theory, which turns out to be an involution operator on the first order Lipschitz classes. Our result generalizes the classical Hardy decomposition of Holder continuous functions on a simple closed curve in the complex plane

Nivel de conocimientos sobre bioseguridad en Estomatología

A cross - sectional descriptive study was carried out to determine the level of knowledge about biosecurity in stomatology in the technical and professional staff of the Julio A. Mella Clinic in Guantanamo province. The universe was constituted by the stomatological personnel who showed willingness to fill out the questionnaire. Knowledge about biosafety was analyzed: (knowledge of Biosafety standards, indications of surface disinfection and instruments, communicable diseases and general evaluation of Biosafety knowledge). An exhaustive bibliographical revision on the subject was carried out with universal and national literatures. The primary data were obtained from a survey sheet prepared by the authors. There were low numbers on a level of knowledge that was moderately sufficient, most of them showed insufficiency.Se realizó un estudio descriptivo transversal con el objetivo de determinar el nivel de conocimiento sobre bioseguridad en Estomatología en  el personal técnico y Profesional de la Clínica “Julio A. Mella” de la provincia Guantánamo. El universo estuvo constituido por el personal estomatológico que mostró disposición en llenar el cuestionario. Se analizaron los conocimientos sobre bioseguridad: (conocimiento sobre las normas de Bioseguridad, indicaciones de desinfección de superficies e instrumental, enfermedades trasmisibles e evaluación en general de los conocimientos sobre Bioseguridad). Se realizó una exhaustiva revisión bibliográfica sobre el tema con literaturas universales y nacionales. Los datos primarios se obtuvieron de una planilla encuesta confeccionada por los autores. Se mostraron cifras bajas sobre nivel de conocimiento medianamente suficiente, la mayoría mostró insuficiencia de estos

Nivel de conocimientos sobre bioseguridad en Estomatología

A cross - sectional descriptive study was carried out to determine the level of knowledge about biosecurity in stomatology in the technical and professional staff of the Julio A. Mella Clinic in Guantanamo province. The universe was constituted by the stomatological personnel who showed willingness to fill out the questionnaire. Knowledge about biosafety was analyzed: (knowledge of Biosafety standards, indications of surface disinfection and instruments, communicable diseases and general evaluation of Biosafety knowledge). An exhaustive bibliographical revision on the subject was carried out with universal and national literatures. The primary data were obtained from a survey sheet prepared by the authors. There were low numbers on a level of knowledge that was moderately sufficient, most of them showed insufficiency.Se realizó un estudio descriptivo transversal con el objetivo de determinar el nivel de conocimiento sobre bioseguridad en Estomatología en  el personal técnico y Profesional de la Clínica “Julio A. Mella” de la provincia Guantánamo. El universo estuvo constituido por el personal estomatológico que mostró disposición en llenar el cuestionario. Se analizaron los conocimientos sobre bioseguridad: (conocimiento sobre las normas de Bioseguridad, indicaciones de desinfección de superficies e instrumental, enfermedades trasmisibles e evaluación en general de los conocimientos sobre Bioseguridad). Se realizó una exhaustiva revisión bibliográfica sobre el tema con literaturas universales y nacionales. Los datos primarios se obtuvieron de una planilla encuesta confeccionada por los autores. Se mostraron cifras bajas sobre nivel de conocimiento medianamente suficiente, la mayoría mostró insuficiencia de estos

RS-1 enhances CRISPR-mediated targeted knock-in in bovine embryos

8 Pág.Targeted knock-in (KI) can be achieved in embryos by clustered regularly interspaced short palindromic repeats (CRISPR)-assisted homology directed repair (HDR). However, HDR efficiency is constrained by the competition of nonhomologous end joining. The objective of this study was to explore whether CRISPR-assisted targeted KI rates can be improved in bovine embryos by exposure to the HDR enhancer RS-1. In vitro produced zygotes were injected with CRISPR components (300 ng/µl Cas9 messenger RNA and 100 ng/µl single guide RNA against a noncoding region) and a single-stranded DNA (ssDNA) repair template (100 ng/µl). ssDNA template contained a 6 bp XbaI site insert, allowing targeted KI detection by restriction analysis, flanked by 50 bp homology arms. Following microinjection, zygotes were exposed to 0, 3.75, or 7.5 µM RS-1 for 24 hr. No differences were noted between groups in terms of development or genome edition rates. However, targeted KI rates were doubled in the group exposed to 7.5 µM RS-1 compared to the others (52.8% vs. 25% and 23.1%, for 7.5, 0, and 3.75 µM, respectively). In conclusion, transient exposure to 7.5 µM RS-1 enhances targeted KI rates resulting in approximately half of the embryos containing the intended mutation, hence allowing direct KI generation in embryos.This study was supported by grants from the Spanish Ministry of Science and Competitiveness (AGL2014‐58739‐R and AGL2017‐84908‐R to PBA), the European Research Council (Starting Grant 757886‐ELONGAN to PBA) and Science Foundation Ireland (13/IA/1983 to PL). ILT was funded by an FPI scholarship from the Spanish Ministry of Science and Competitiveness (BES2015‐072774) and AMM by an industrial doctorate scholarship from Madrid Region Government (IND2017/BIO7748).Peer reviewe