Efficient targeting to storage granules of human proinsulins with altered propeptide domain.

In neuronal and endocrine cells, peptide hormones are selectively segregated into storage granules, while other proteins are exported continuously without storage. Sorting of hormones by cellular machinery involves the recognition of specific structural domains on prohormone molecules. Since the propeptide of insulin is known to play an important role in its three-dimensional structure, it is reasonable to speculate that targeting of proinsulin to storage granules would require a functional connecting peptide. To test this hypothesis, we constructed two mutations in human proinsulin with different predicted structures. In one mutation, Ins delta C, the entire C peptide was deleted, resulting in an altered insulin in which the B and the A chains are joined contiguously. In the other mutation, Ins/IGF, the C peptide of proinsulin was replaced with the unrelated 12-amino acid connecting peptide of human insulin-like growth factor-I; this substitution should permit correct folding of the B and A chains to form a tertiary structure similar to that of proinsulin. By several biochemical and morphological criteria, we found that Ins/IGF is efficiently targeted to storage granules, suggesting that the C peptide of proinsulin does not contain necessary sorting information. Unexpectedly, Ins delta C, which presumably cannot fold properly, is also targeted to granules at a high efficiency. These results imply that either the targeting machinery can tolerate changes in the tertiary structure of transported proteins, or that the B and A chains of insulin can form a relatively intact three-dimensional structure even in the absence of C peptide

A Toom rule that increases the thickness of sets

Toom's north-east-self voting cellular automaton rule R is known to suppress small minorities. A variant which we call R^+ is also known to turn an arbitrary initial configuration into a homogenous one (without changing the ones that were homogenous to start with). Here we show that R^+ always increases a certain property of sets called thickness. This result is intended as a step towards a proof of the fast convergence towards consensus under R^+. The latter is observable experimentally, even in the presence of some noise.Comment: 16 pages, 8 figure

QCD with light Wilson quarks on fine lattices (II): DD-HMC simulations and data analysis

In this second report on our recent numerical simulations of two-flavour QCD, we provide further technical details on the simulations and describe the methods we used to extract the meson masses and decay constants from the generated ensembles of gauge fields. Among the topics covered are the choice of the DD-HMC parameters, the issue of stability, autocorrelations and the statistical error analysis. Extensive data tables are included as well as a short discussion of the quark-mass dependence in partially quenched QCD, supplementing the physics analysis that was presented in the first paper in this series.Comment: TeX source, 35 pages, figures include

Pushing the limits: K2 observations of the trans-Neptunian objects 2002 GV31 and (278361) 2007 JJ43

We present the first photometric observations of trans-Neptunian objects (TNOs) taken with the Kepler space telescope, obtained in the course of the K2 ecliptic survey. Two faint objects have been monitored in specifically designed pixel masks that were centered on the stationary points of the objects, when their daily motion was the slowest. In the design of the experiment, only the apparent path of these objects were retrieved from the detectors, i.e. the costs in terms of Kepler pixels were minimized. Because of the faintness of the targets we employ specific reduction techniques and co-added images. We measure rotational periods and amplitudes in the unfiltered Kepler band as follows: for (278361) 2007 JJ43 and 2002 GV31 we get P_rot=12.097 h and P_rot=29.2 h while 0.10 and 0.35 mag for the total amplitudes, respectively. Future space missions, like TESS and PLATO are not well suited to this kind of observations. Therefore, we encourage to include the brightest TNOs around their stationary points in each observing campaign to exploit this unique capability of the K2 Mission -- and therefore to provide unbiased rotational, shape and albedo characteristics of many objects.Comment: Accepted for publication in ApJ Letters, 5.2 pages in emulateapj style, misspelled 2007 JJ43 designation correcte

The Prevalence of Workaholism: A Survey Study in a Nationally Representative Sample of Norwegian Employees

Workaholism has become an increasingly popular area for empirical study. However, most studies examining the prevalence of workaholism have used non-representative samples and measures with poorly defined cut-off scores. To overcome these methodological limitations, a nationally representative survey among employees in Norway (N = 1,124) was conducted. Questions relating to gender, age, marital status, caretaker responsibility for children, percentage of full-time equivalent, and educational level were asked. Workaholism was assessed by the use of a psychometrically validated instrument (i.e., Bergen Work Addiction Scale). Personality was assessed using the Mini-International Personality Item Pool. Results showed that the prevalence of workaholism was 8.3% (95% CI = 6.7–9.9%). An adjusted logistic regression analysis showed that workaholism was negatively related to age and positively related to the personality dimensions agreeableness, neuroticism, and intellect/imagination. Implications for these findings are discussed

Evidence for fresh frost layer on the bare nucleus of comet Hale--Bopp at 32 AU distance

Here we report that the activity of comet Hale--Bopp ceased between late 2007 and March, 2009, at about 28 AU distance from the Sun. At that time the comet resided at a distance from the Sun that exceeded the freeze-out distance of regular comets by an order of magnitude. A Herschel Space Observatory PACS scan was taken in mid-2010, in the already inactive state of the nucleus. The albedo has been found to be surprisingly large (8.1$\pm$0.9%{}), which exceeds the value known for any other comets. With re-reduction of archive HST images from 1995 and 1996, we confirm that the pre-perihelion albedo resembled that of an ordinary comet, and was smaller by a factor of two than the post-activity albedo. Our further observations with the Very Large Telescope (VLT) also confirmed that the albedo increased significantly by the end of the activity. We explain these observations by proposing gravitational redeposition of icy grains towards the end of the activity. This is plausible for such a massive body in a cold environment, where gas velocity is lowered to the range of the escape velocity. These observations also show that giant comets are not just the upscaled versions of the comets we know but can be affected by processes that are yet to be fully identified.Comment: 7 pages, 6 figures, accepted for publication in Ap