Genotypic properties of collection plague microbes strains from the natural plague foci of Kazakhstan

Background. Epidemiological and epizootological monitoring of natural plague foci requires an integrated approach to solving problems, taking into account the phenotypic and genetic variability of Y. pestis and zoning of natural plague foci. The introduction of a new molecular genetic methodology aimed at studying the genomic polymorphism of the plague pathogen provides reliable results for the differentiation of not only groups, but also individual strains.The aim. To determine the genotypes of the plague microbe from different autonomous foci of the Republic of Kazakhstan.Materials and methods. 105 strains of Y. pestis isolated from various natural plague foci of Kazakhstan in 1951–2015 were studied. The phenotypic properties of the strains were studied using standard microbiological methods. A polymerase chain reaction (PCR) was used to detect fragments of the cafl, pst and YPO2088 genes. Multilocus variable number tandem repeat (VNTR) analysis (MLVA) was performed for 25 VNTR loci.Results. The phenotypic properties of the strains were preliminarily studied and the strains of the plague microbe were tested for specificity using the Pest-Quest test system (Kazakhstan). The PCR study confirmed the species-specific affiliation of Y. pestis strains. A variety of strains with typical phenotypic characteristics was revealed. MLVA for 25 key loci (MLVA25) revealed that the studied strains of the plague microbe are phylogenetically closest to the Mediaevalis biovar representatives. A phylogenetic tree of the studied strains has been obtained. It was found that 9 genotypes circulate on the territory of Kazakhstan, and their distribution in certain natural plague foci was determined.Conclusions. The resulting clustering indicates the relationship between the strain groups obtained on the dendrogram by the MLVA25 method and the territories of certain natural plague foci

Biological Properties and Molecular Genetic Characteristics of Bacillus Anthracis Strains Isolated During Anthrax Outbreaks in Kazakhstan in 2016

Today, anthrax is recorded in many countries around the world, in Kazakhstan – in the form of sporadic cases or small outbreaks. Despite the endemicity of anthrax in Kazakhstan, the genetic diversity strains is not well described. At the moment, MLVA-25, which is sufficient for molecular and epidemiological monitoring, is recognized as one of the most discriminatory methods of genotyping.Objective: to determine the cultural and morphological properties, the genomic characteristics of the strains of the anthrax pathogen, their geographical distribution in the territory of Kazakhstan during the outbreaks in 2016, a comparative analysis with collection strains isolated since 1962.Methods: microbiological, genetic research methods were used in the work.Results. We investigated 11 strains of B. anthracis, which were isolated in 2016 in Kazakhstan. For comparison of genotypes, 26 strains were used from the collection of pathogenic microorganisms of the A.M. Aykimbaev’s Kazakh Scientific Center for Quarantine and Zoonotic Diseases. Phylogenetic analysis clustered 37 strains of B. anthracis into three clusters and 23 genotypes.The results of studying the phenotypic properties of anthrax strains by the main identification tests showed that all the strains studied in the experiment and isolated from 1961 to 2016 had biological properties characteristic of typical of B. anthracis strains.The anthrax strains isolated in 2016 are characterized as significant variability, as well as the circulation of the same genotypes and clusters in different areas of Kazakhstan. MLVA-profiles of analyzed Kazakhstan strains are unique and do not fully coincide with any studied strain from MLVAbank. On the MST-tree, Kazakhstan’s strains are located in three clusters, as on the phylogenetic tree.Conclusion: Molecular genetic analysis of B. anthracis strains enhances the ability of epidemiologists to track the sources and pathways of infection.It is necessary to improve the tracking system for strains of especially dangerous infections in  Kazakhstan using modern molecular genetic methods

Retrospective analysis and modern spatiotemporal characteristics of tularemia in the territory of the West Kazakhstan and North Kazakhstan regions

Rationale. An important task is to monitor the incidence of tularemia among the population of Kazakhstan. Natural foci of this infection occupy large areas. In some regions with large numbers of rodents and ectoparasites and low vaccination coverage, human cases of tularemia have been reported.The aim of the study. To carry out retrospective analysis and to study modern spatiotemporal characteristics of tularemia in the West Kazakhstan and North Kazakhstan regions in order to improve the effectiveness of preventive measures.Materials and methods. In our work, we used public records, the results of an epizootological survey of tularemia natural foci and the official data from the Departments of Sanitary and Epidemiological Control of two regions on the human cases of tularemia in 2000–2021. We used descriptive statistics methods, relative and absolute indicators of the tularemia incidence in the population for the analysis. The phenotypic and genetic properties of the strains isolated in 2000–2021 were studied according to the guidelines.Results. A retrospective analysis of the tularemia incidence among the population of the North Kazakhstan and West Kazakhstan regions showed an improvement in the epidemic situation. Over the past 20 years, 4 human cases of tularemia have been registered in the West Kazakhstan region, while the epizootic potential was quite high; more than 300 strains of the tularemia microbe were isolated during the studied period. In the North Kazakhstan region from 2000 to 2021, 11 human cases of tularemia were registered; when studying rodents, mammals and environmental objects, single positive samples for specific tularemia antibodies and antigens were detected; no strains of tularemia microbe were isolated.Conclusion. An analysis of long-term data on the epizootic and epidemic activity of tularemia natural foci, processed using descriptive statistics and GIS technology, made it possible to identify places of long-term persistence of the tularemia agent in the natural focus of the North Kazakhstan and West Kazakhstan regions and to create an electronic map of the territories endemic for tularemia to determine the scope of preventive measures

Anthrax in the East Kazakhstan Region

Almost every year in Kazakhstan, cases of diseases of animals and people with anthrax are recorded. The incidence rate of people with anthrax in the period from 2000 to 2018 ranged from 0.01 to 0.24 per 100 thousand people. In the territory of the East Kazakhstan region, the incidence rate is higher than in the republic.Objective: a retrospective analysis of anthrax outbreaks in the East Kazakhstan region from 2000 to 2018.Methods: a retrospective analysis using statistical, cadastral data, archival funds, the results of participation in the investigation of outbreaks of infection.Results. Since 1938, cases of animal and human disease of anthrax have been recorded in East Kazakhstan. Infection of humans occurs during the slaughter of animals. In Zharma, Urjar, and Ayagozskiy areas most of all are anthrax foci of soil. In 67 % of cases, outbreaks of anthrax were recorded in these areas.Conclusion. The relative incidence rate of anthrax in people in East Kazakhstan is from 0.07 to 0.27, since 2001 it is higher than the republican indicator.In the East Kazakhstan region from 1997 to 2018, 37 people fell ill with anthrax. The form of the disease is skin, in 8.1 % secondary sepsis. In the remaining patients, the diseases were mild (70.3 %), ended in recovery. The source of human infection is mainly cattle. The isolated strains of B. anthracis have typical properties, they are included in cluster A1a, A3b (MLVA-8). The strains isolated in 2016 are similar to the strains isolated in the Almaty region (MLVA-25). Grouped with a number of European, Asian and African strains from France, Germany, Italy, Tajikistan, Pakistan, Korea and Namibia. The presence of a significant number of soil foci of anthrax in the territory of the East Kazakhstan region, not fully the implementation of veterinary and sanitary preventive measures leads to an exacerbation of the situation for anthrax