Community-acquired pneumonia: the dynamics of senior medical students’ knowledge

Сommunity-acquired pneumonia (CAP) is an acute infectious disease. Inflammation of the lower respiratory system, interstitium, and microvasculature, mainly of bacterial etiology, is a typical pathological sign of this disease. It is one of the leading causes of death from infectious diseases. The survey was conducted within the KNOCAP multicenter research project (the full name of the project “The assessment of students' and physicians' knowledge of community-acquired pneumonia basics”

BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA

The emergence of resistance among the most important bacterial pathogens is generally recognized as one of the major public health problems. The most important of these organisms are penicillin-resistant Streptococcus pneumoniae, vancomycin-resistant enterococci and methicillin- and vancomycin-resistant Staphylococcus aureus. These antibiotic resistance in common pathogens have made antimicrobial therapy of many infections. Scientists need to look for new ways of treating bacterial infections in the work, using the developed algorithm from the methods of search software in the genomic structure of Staphylococcus aureus subsp. aureus ST228, the CRISPR/Cas locus and the division structures of its CRISPR cassette. The results of the bacteriophage search through the decoded spacer sequences of CRISPR-cassettes of this strain were also obtained using the developed algorithm of the software methods of bioinformatics. It was determined that the CRISPR/Cas system of strain of ST228 of S. aureus was of type IIIA. It is shown that cas-genes are in the immediate vicinity of CRISPR cassettes. The spacer structures in the detected CRISPR cassette are the Staphylococcus, Mycobacterium, Streptococcus, Bacillus, Gordonia, Arthrobacter, Streptomyces. The implementation of the algorithm of program methods for locating CRISPR/Cas-loci can be applied to many other decoded bacterial genomes to return bacteriophage therapy

Using the Analysis of Public Opinion on the Situation, System Diagnostics Problem Areas

In accordance with the methodology of system diagnostics of regional situations statistical portrait of the North Caucasus republics is combined with the results of the analysis specifically public opinion poll in the republics, which revealed their real socio- economic problems