Assessment of Stability of Chemical Cholera Vaccine in a New Primary Packaging

The bivalent chemical cholera vaccine is the only drug for the prevention of cholera registered in the Russian Federation. The vaccine has been produced in glass bottles containing 210 tablets. At the same time, modern trends dictate the need to produce the drug in varying dispensing and more practical packaging for the convenience of the consumer.The aim of the work was to study the stability of the properties of the immunobiological medicinal product “Bivalent chemical cholera vaccine” with modified filling and in new primary packaging.Materials and methods. When studying the quality of bivalent chemical cholera vaccine batches, physicochemical parameters, formaldehyde content, specific activity and safety, abnormal toxicity, immunogenicity, and microbiological purity were assessed. Stability in terms of “specific activity” was evaluated using dot immunoassay.Results and discussion. As a result of this work, the use of several dispensing options and new primary packaging of cholera vaccine has been experimentally substantiated. The stability of the finished vaccine preparation has been established in the “accelerated aging” test and during long-term storage. The possibility of using dot immunoassay with a conjugate based on staphylococcal protein A, labeled with colloidal gold, to monitor the stability of cholera vaccine has been experimentally demonstrated

INFLUENCE OF APOE GENOTYPES ON BIOCHEMICAL INDICES OF IRON AND LIPID METABOLISM IN PATIENTS WITH THE ENDOMETRIUM NEOPLASTIC DISEASES AND HEAL THY WOMEN

Objective. APOE genotype association with the level of iron in plasma and the risk of endometrial neoplastic diseases has not been studied. The aim of the work was to analyze the genotypic associations of the apolipoprotein E (APOE) with iron and lipid levels in endometrial hyperplasia (EH)/endometrial cancer (EC) patients and healthy women.Materials and methods. The biochemical blood levels of iron and cholesterol metabolism detected in women d with diagnosed endometrial hyperplasia (53), endometrial cancer (87) and healthy women (70) are determined on an automated analyzer «OLYMPUS AU – 400”. The genotyping was performed with a PCR-RFLP (polymerase chain reaction with restriction fragment length polymorphism) technique.Results. A statistically significant decrease in the levels of serum iron, transferrin saturation, high-density lipoprotein on the one hand, and a statistically significant increase in body mass index, triglycerides, very low density lipoproteins, atherogenic index in patients with endometrial adenocarcinoma was observed. Statistically significant differences in the distribution of E3, E4, E2 allele frequencies between patients and healthy women (P = 0.048) was revealed. E3 allele frequency was reduced, and the E2 allelewas increased in patients with EC. It was shown that the serum iron level was significantly increased in EC patients and healthy carriers of the genotype E3/E2 (P= 0,0014 and 0,0363 respectively). There was significantly elevated triglyceride level in carriers of the minor genotypes E2/E4, E4/E4 among patients with EH (0,0389).Conclusions. Genotype E3/E2 compared with other APOE genotypes under study is associated with elevated levels of iron and the risk of endometrial cancer. The association APOE 2/2, 2/4, 4/4 as well as 3/2 with endometrial neoplastic diseases requires further investigation. The data obtained can be the basis for the creation of a marker system for determining a high level susceptibility to malignant disease of the female reproductive system

ON-LINE PLATFORM FOR COMPETENCES DEVELOPMENT IN CORPORATE EDUCATIONAL SYSTEMS

The aim of the study is to present the model of interaction of university and business corporations in uniform information and educational space, which consists of the uniform requirements to the contents and quality of education and requirements of science; education and business are equally satisfied.Methods. The methods involve summing up the results of the national and international researchers’ findings on the problem of vocational pedagogical education; methods of expert survey and system analysis.Results. The information research results disclosing the drawbacks of the existing online-education technologies are presented. There is also a presentation of online-platform concept «The University of the 3rd Millennium» where modern university is considered as a service provider responsible for corporate education. Outsourcing will allow the companies to cut their expenses on employee education programs, while universities will accumulate additional resources by means of developing and selling innovative educational products. The additional funds will enable the university to carry out scientific research, to effectively implement post graduates’ employment programs, to introduce innovations.Scientific novelty. The authors define «as is» competences, which the university must have to launch this online-platform project, and «as to be» competences, which every university is to master to successfully implement the project. Internal high-risk systemic tensions and external challenges facing the platform are defined; strategic matrix of their intersection is developed. The latter allowed the authors to specify essential tasks which demand solution within the project. There is also a description of interconnection of the defined tasks with the «as to be» competences, and a Canvas model of the suggested online platform.Practical significance: The authors of the article propose the model of business and education interaction, within which state institutions and local authorities, enterprises and companies with different patterns of ownership will pass corporate education to a modern university, effectively fulfilling the functions of a research, educational and consultative center in many fields.The research results proving readiness of the heads of the companies and organizations (Moscow, St.-Petersburg, Lipetsk, Chelyabinsk, Kostroma) to outsource their corporate education to universities prove that the offered solution is more appropriate than the existing alternatives (systems of additional education in universities, MOOC, corporate universities)