Virus and bacterial associations verified in the urine of healthy subjects (pilot study)

Introduction. CCurrently, there are more and more new data that the urine of the healthy subject has its own unique microbiota and virobiota. Nevertheless, in the etiology and pathogenesis of the inflammatory diseases of the urinary system, the bacterial component is the most studied, but the viral component, as a rule, remains outside the scope of the standard clinical examination of patients. Objectives. To investigate the viral-bacterial associations in the urine of healthy subjects.Materials and methods. The 20 healthy sexually active women and men were examined, which are divided into groups according to gender: Group I – women (n = 10), Group II – men (n = 10). The average age of the subjects was 22.4 ± 1.2 years.Results. Bacteriological examination showed that in the urine of healthy women predominates Lactobacillus spp. (90,0%), Peptococcus spp. (80.0%), Propionibacterium spp. (70.0%), and in the urine of healthy men - Eubacterium spp. (70.0%) and Peptostreptococcus spp. (40.0%). During the polymerase chain reaction of urine were detected the papilloma (HPV) and herpetic (HSV) viruses in 40.0% of cases. In group I were verified HPV (20.0%) and HSVII (10.0%), in group II was found only the HPV (10.0%). In all cases, when viruses were detected in the urine, they were recorded as part of virus-bacterial associations. In one healthy woman in the urine were found HPV + HSVII associationsConclusions. The findings about of verification different taxa of viruses in the urine of healthy subjects are the basis for understanding and detailing the etiological structure of infections of the urinary system. The further studies should be aimed for increasing the cohort of healthy subjects in order to obtain the correct factograms of bacterial and viral patterns which present in their urine