Операционный метод построения функций грина для малых времен, сооветствующих решению краевых задач для уравнений переноса параболического типа

This article is dedicated to the expansion of mathematical tools used to solve the problems of thermodynamics, in particular, problems in the theory of thermal shock. The authors used the method of Green's functions, which allowed simplifying the solution of the relevant problems.Настоящая статья посвящена расширению математического аппарата, используемого для решения задач термодинамики, в частности, задач теории теплового удара. Авторами был использован метод функций Грина, что позволило существенно упростить решение соответствующих задач

FEATURES OF IMMUNE RESPONSE IN PRETERM BABIES WITH BRONCHOPULMONARY DISPLASIA OF VARIOUS SEVERITY

To evaluate the immunity indexes in the children who were born with bronchopulmonary dysplasia (BPD) of varying severity at very early delivery terms (22-27 weeks), dynamic examinations were performed in 35 infants: 17 children were with severe BPD; 18, with mild-severity and moderately severe BPD. The comparison group consisted of seven children born at 22-27 weeks of gestational age without signs of BPD. Relative numbers of lymphocyte subpopulations (CD3+, CD4+, CD8+, CD19+, CD16+CD56+, CD4+CD25+), and monocytes (CD14+CD64+, CD14+HLA-DR+) were determined by flow cytometry. The level of cytokines (IL-6, IL-8, IL-4) was measured by enzyme immunoassay technique. The features of immune status in children with BPD of severe, mild and moderate severity were discerned. It was found that the predictors of severe BPD development in the children born at very early terms, are: increased content of IL-8 at birth and at the age of 1 month, reduced level of expression on monocytes (CD64, HLA-DR) on the 1 month of life, and CD14+CD64+ cells at 38-40 weeks post conception. The revealed features of immune status in newborns with BPD can be used to assess the effectiveness of the therapy, which requires further research in this direction

Molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosis

Mycoplasmas are bacteria that are extremely unstable in vitro as they lack a rigid cell wall. They are most often detected in association with other pathogens, including those that can become L-forms if treated with antibiotics. Mycoplasma colonies, as well as colonies of L-form bacteria, have a typical «fried egg» appearance, therefore it is necessary to differentiate them for the accurate diagnosis and choice of treatment. The paper presents data on mycoplasma infection diagnosis in cattle and results of differentiation of isolated mycoplasma and L-form bacteria colonies using multiple passaging and real-time polymerase chain reaction. For that, 177 samples were collected from animals with mycoplasmosis clinical signs, 45 of them were tested using molecular genetic method, 132 samples were subjected to bacteriological testing. Mycoplasma DNA was detected in 71.1% of samples, and specific colonies were detected in 3.8% of samples. Such biochemical tests of mycoplasma species identification as arginine hydrolysis, blood serum liquefaction, film and grain formation, inoculation into Tween-80-containing medium, hemadsorption and hemolysis of erythrocytes do not allow an objective assessment of the species belonging to mycoplasmas, but, according to the results obtained, the isolated species most likely belongs to Mycoplasma dispar, which is pathogenic for cattle. Real-time polymerase chain reaction is undoubtedly the most accurate and rapid diagnostic method for mycoplasmosis, but a preliminary diagnosis can also be established bacteriologically within 2–7 days. In addition, during microbiological testing, it is possible to assess the antibiotic resistance of mycoplasma isolates, thereby developing an optimal and high-quality scheme of the disease treatment and prevention

Comparative characteristic of parameters of umbilical and peripheral blood in dependence on type of delivery

Clinical-immunological adaptation was analyzed of 28 newborn delivered by cesarion section and 42 neonatal infants born by vaginal delivery. At operation general anesthesia was used with nitrous oxide. It has been ascertained that hematological and immunological parameters may be used as additional criteria for early diagnosis of impaired clinical adaptive processes of newborn.Проведен сравнительный анализ клинико-иммунологической адаптации 28 новорожденных, извлеченных путем кесарева сечения и 42 новорожденных, родившихся естественным путем. При операции применялась общая анестезия закисью азота. Установлено, что гематологические и иммунологические показатели могут использоваться в качестве дополнительных критериев ранней диагностики нарушений адаптации у новорожденных

Efficacy evaluation of the intravaginal preparation containing lactobacilli culture in the case of recurrent vulvovaginal candidiasis

То determine the effectiveness of the recurrence vulvovaginal candidiasis prevention by lactobacilli drugs within a multicenter international study at Mother and Child Care Research Institute in Russian Public Health Ministry, follow-up of 30 patients be carried out. After antimycotic therapy using a Sertaconazole, female patient from main group (15 women) received intravaginal lactobacillus preparation 'Lactoginal' within 21 days; a comparison group of patients did not receive the probiotic. The period of dynamic observation up of patients was 5 months, during which carried out registration of exacerbations (reported and laboratory-confirmed). These results demonstrate the effectiveness of combined therapy using Sertaconazole (Zalain) followed by the application Lactoginal within 21 days to prevent recurrence of vulvovaginal candidiasis compared to monotherapy with Sertaconazole.С целью определения эффективности профилактики рецидивов вульвовагинального кандидоза препаратами лакто-бацилл в рамках многоцентрового международного исследования в ФГБУ «НИИ ОММ» наблюдалось 30 пациенток. После проведения антимикотинеской терапии сертаконазолом, пациентки основной группы (15 женщин) получали интравагинально препарат лактобацилл «Лактожиналь» в течение 21 дня; пациентки группы сравнения не получали пробиотик. Период динамического наблюдения за пациентками составил 5 месяцев, во время которого проводилась регистрация обострений (релортированных и лабораторно подтвержденных). Представленные результаты демонстрируют эффективность использование комплексной терапии сертаконазолом (Залаин) с последующим применением прерапата Лактожиналь в течение 21 дня с целью профилактики рецидива вульвовагинального кандидоза по сравнению с монотерапией препаратами сертаконазола

Prognostic criteria of the formation of neurological pathology in the neonatal period for the childrenborn by cesarean section

Aim of study: To determine prognostic markers of the risk of realization of perinatal pathology of central nervous system (CNS) for the children born by Caesarean sections. Materials and methods: A biochemical and immunological study of umbilical cord blood of the newborn children born by Caesarean sections with the perinatal pathology CNS (n=28) and without perinatal pathology CNS (n=15) was carred out.Results and discussion: It was found that the cord blood of babies born by surgery and have developed a neonatal neurological pathology, there is a significant decrease in the level ofpH, partial pressure of oxygen content of the true and standard bicarbonate, and the increase in the deficit of buffer bases of whole blood and interstitial liquid, high concentrations of IL-1ß, IL-4, IL-6, IL-8, TNF-a, neurospecifi cenolazy, compared with to analogical indexes of infants without neurological pathology. Prognostic markers (standard bicarbonate, IL-6, IL-8), reflecting the development of neurological pathology in the neonatal period were identified.Цель исследования: определение прогностических маркеров риска реализации перинатального поражения ЦНС в неонатальном периоде у детей, родившихся путем операции кесарева сечения. Материалы и методы: проведено биохимическое и иммунологическое исследование пуповинной крови новорожденных детей, родившихся путем операции кесарева сечения, с реализованным перинатальным поражением ЦНС (п=28) и не имеющих перинатального поражения ЦНС (п=15). Результаты и обсуждение: установлено, что в пуповинной крови детей, родившихся оперативным путем и развивших в неонатальном периоде неврологическую патологию, отмечается достоверное снижение уровня pH, парциального давления кислорода, содержания истинного и стандартного бикарбоната, а также увеличение дефицита буферных оснований цельной крови и межклеточной жидкости, повышенное содержание IL-1ß,IL-4, IL-6, IL-8, TNF-a, нейро-специфической енолазы, по сравнению с аналогичными показателями новорожденных, не имеющих неврологической патологии. Выделены прогностические маркеры (стандартный бикарбонат, IL-6, IL-8) отражающие развитие неврологических нарушений в неонатальном периоде

Effect of the antihypertensive therapy used during pregnancy on features of the adaptation period in the infants

The aim of the research: to evaluate the effect of the different variants of the antihypertensive therapy during pregnancy on clinical features of the adaptation period and the state of the metabolic parameters in the infants. The study, including 65 infants of women with chronic hypertension used during pregnancy antihypertensive therapy. Higher anthropometric measures, the low percentage of LUGR, the low incidence of disease, the earlier statements were typical for infants of women who received monotherapy dopegyt during pregnancy. The low anthropometric measures, the high percentage of LUGR, the high incidence of disease, the trend toward more frequent neurological disorders are occurring in the newborns of the mothers who received combination therapy dopegyt and calcium channel blockers. Hemodynamic adaptation proceeded somewhat less favorable in the infants whose mothers received antihypertensive treatment β-blockers.Цель исследования: оценить влияние использования различных вариантов антигипертензивной терапии во время беременности на клинические особенности течения адаптационного периода и состояние некоторых параметров метаболизма у новорожденных. Под наблюдением находились 65 новорожденных от женщин, страдающих хронической артериальной гипертензией и получавших во время беременности антигипертензивную терапию. Для детей от женщин, получавших во время беременности монотерапию допегитом, характерны были высокие антропометрические показатели, низкий процент СЗРП, низкая частота заболеваемости, более ранние сутки выписки. Сниженные антропометрические показатели, высокий процент СЗРП, повышенная заболеваемость, тенденция к более частой неврологической патологии отмечались в группах новорожденных от матерей, находившихся на комбинированной терапии, причем при сочетании допегита с блокаторами кальциевых каналов данные показатели были несколько хуже, чем при сочетании β-адреноблокаторов с блокаторами кальциевых каналов. Гемодинамическая адаптация наименее благоприятно протекала у новорожденных, матери которых получали антигипертензивную терапию β-адреноблокаторами

Operational method for constructing Green’s functions for small times corresponding to the solution to the boundary value problems for transfer equations of parabolic type

This article is dedicated to the expansion of mathematical tools used to solve the problems of thermodynamics, in particular, problems in the theory of thermal shock. The authors used the method of Green's functions, which allowed simplifying the solution of the relevant problems

Morphology of mammary gland lactating goats in age aspect

A morphological study of the mammary gland of lactating goats held at the Department of anatomy and physiology of Kostroma SAA in 2010—2011. It is established, that the mammary glands are undergoing significant changes in age aspect