DIAGNOSTIC POTENTIAL OF THE ERYTHROCYTIC IMMUNOGLOBULIN DIAGNOSTICUM FOR INDICATION AND IDENTIFICATION OF THE CAUSATIVE AGENTS OF PARTICULARLY DANGEROUS (DEEP) MYCOSES

Objective of the study was to assess analytical and diagnostic sensitivity and specificity of the “Reagent kit. Erythrocytic coccidioidomycosal and histoplasmosal immunoglobulin dry diagnosticum”, designed for identification of causative agents of coccidioidomycosis and histoplasmosis in isolated cultures of micromycetes, as well as in clinical and biological samples using indirect hemagglutination test.Materials and methods. The investigation included 264 positive samples (216 samples of micromycete suspensions, 48 samples of biological and clinical material) containing pathogens of histoplasmosis and coccidioidomycosis concentrated up to 3,12·106 and 1,56·106 cells/ml, respectively, and 128 negative samples containing heterologous microorganisms in concentrations equal to 5·106 cells/ml. The study was carried out using biological samples that were artificially contaminated with stated pathogens of particularly dangerous mycoses and samples, obtained from bioassay animals with experimental infection.Results and conclusions. It is established that diagnostic sensitivity of the reagent kit is not less than 99,0 %. The diagnostic specificity is not less than 98,0 %. Reproducibility of the results in all cases was 100 %. The results obtained testify to the prospect of introduction of the developed kit into the health care practice

System of Monitoring and Response to Public Health Emergencies of Sanitary-Epidemiological Character in the CIS Countries

The most important component of strengthening the potential for responding to biological threats both at the national and interstate levels is the formation of a unified system for monitoring and responding to emergencies (ES) of sanitary-epidemiological nature in the CIS territory.The aim of the work was to review the systems for monitoring and responding to emergencies of sanitary-epidemiological character in the CIS countries by the example of the Russian Federation, the Republic of Belarus, the Republic of Kazakhstan and the Kyrgyz Republic, to characterize the main areas of international cooperation on countering biological threats and coordinating international response measures in the CIS countries.Materials and methods. Information and analytical materials provided by organizations responsible for epidemiological surveillance and control in the CIS countries, Internet sources, and publications were used for the study.Results and discussion. The organization and functioning of the systems for monitoring and responding to emergencies in the CIS countries is a state function. It includes, as a rule, the national, regional (sub-national) and territorial (local) levels, which have horizontal and vertical connections. The legal framework is made up of documents of the legislative level. Interdepartmental interaction in response to emergencies is carried out both at the republican level and in administrative territories; the basis for interaction is the integrated planning of preventive and anti-epidemic measures and the functioning of the relevant organizational structures on an ongoing basis. Since 2015, with the support of the Government of the Russian Federation, programs have been implemented aimed at assisting partner countries in the implementation of the International Health Regulations (2005) in order to increase national response capacity and form a unified sanitaryepidemiological emergency response system in the CIS countries. The main areas of collaboration are strengthening the material and technical base and human resources of specialized institutions and scientific cooperation. As a result of the program implementation, a unified system for monitoring and prompt response to emergencies in the field of public health of sanitary-epidemiological nature has essentially been formed in the CIS countries to date, uniting more than 15 specialized institutions from 8 CIS countries

Management of the Investigation and Detection of Legionellosis Agent in the Environmental Samples during the Preparation and Holding of the XXII Winter Olympics and XI Paralympics in Sochi

Provided are the data on the management and carrying out of the laboratory control over the hot-water supply systems of the sports venues and accommodation areas for the presence of Legionella pneumophila during the XXII Olympics and XI Paralympics in Sochi, 2014. Inspected have been 105 facilities. The samples from 37 of them showed positive. L. pneumophila DNA concentration varies between 2.19·102 and 3.92·107 genome equivalents (g.e.)/l. By means of bacteriological investigation detected have been legionella colonies in the water supply systems of 16 facilities, at one of the items - the loading is over 1·104 colony-forming units/l. Performed has been comparative analysis of the results obtained using PCR assay and bacteriological test. Based on the genotyping of 7 isolated strains serogroup 1 and 2 strains of 2-14 serogroups it is concluded that L. pneumophila strains circulating in the resort town Sochi are genetically heterogeneous