Genome wide analysis of gene expression changes in skin from patients with type 2 diabetes

Non-healing chronic ulcers are a serious complication of diabetes and are a major healthcare problem. While a host of treatments have been explored to heal or prevent these ulcers from forming, these treatments have not been found to be consistently effective in clinical trials. An understanding of the changes in gene expression in the skin of diabetic patients may provide insight into the processes and mechanisms that precede the formation of non-healing ulcers. In this study, we investigated genome wide changes in gene expression in skin between patients with type 2 diabetes and non-diabetic patients using next generation sequencing. We compared the gene expression in skin samples taken from 27 patients (13 with type 2 diabetes and 14 non-diabetic). This information may be useful in identifying the causal factors and potential therapeutic targets for the prevention and treatment of diabetic related diseases

ÉTUDE BIOMÉTRIQUE DES BOVINS DE BOUCHERIE. III. - VARIABILITÉ DE LA COMPOSITION EN MORCEAUX ET DES CARACTÈRES DE CONFORMATION DE LA CARCASSE DES BOEUFS CHAROLAIS

International audienc

ÉTUDE BIOMÉTRIQUE DES BOVINS DE BOUCHERIE. II - ESTIMATION DU POIDS DE LA MUSCULATURE CHEZ LES BOVINS CHAROLAIS

International audienc

ÉTUDE DE LA PRODUCTION DE LA VIANDE CHEZ LES OVINS. IV. RELATION ENTRE LA FORME ET LA COMPOSITION DU MEMBRE POSTÉRIEUR

International audienc

ETUDE BIOMETRIQUE DES BOVINS DE BOUCHERIE. I. - VARIABILITÉ DE LA COMPOSITION ANATOMIQUE DE LA CARCASSE DES BOVINS CHAROLAIS

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Perivascular-resident macrophage-like melanocytes in the inner ear are essential for the integrity of the intrastrial fluid–blood barrier

The microenvironment of the cochlea is maintained by the barrier between the systemic circulation and the fluids inside the stria vascularis. However, the mechanisms that control the permeability of the intrastrial fluid–blood barrier remain largely unknown. The barrier comprises endothelial cells connected to each other by tight junctions and an underlying basement membrane. In a recent study, we found that the intrastrial fluid–blood barrier also includes a large number of perivascular cells with both macrophage and melanocyte characteristics. The perivascular-resident macrophage-like melanocytes (PVM/Ms) are in close contact with vessels through cytoplasmic processes. Here we demonstrate that PVM/Ms have an important role in maintaining the integrity of the intrastrial fluid–blood barrier and hearing function. Using a cell culture-based in vitro model and a genetically induced PVM/M-depleted animal model, we show that absence of PVM/Ms increases the permeability of the intrastrial fluid–blood barrier to both low- and high-molecular-weight tracers. The increased permeability is caused by decreased expression of pigment epithelial-derived factor, which regulates expression of several tight junction-associated proteins instrumental to barrier integrity. When tested for endocochlear potential and auditory brainstem response, PVM/M-depleted animals show substantial drop in endocochlear potential with accompanying hearing loss. Our results demonstrate a critical role for PVM/Ms in regulating the permeability of the intrastrial fluid–blood barrier for establishing a normal endocochlear potential hearing threshold