Diagnostic and prognostic significance of some inflammatory serum proteins in patients with precancerous diseases and cervical cancer

Background: The high social significance of cervical cancer, the shortcomings of the performed cervical screening is prerequisites for research in the field of mproving the diagnosis of this disease. We hypothesized that the systemic level of some inflammatory proteins could be used as a diagnostic criterion for cervical cancer.The aim of the study was to study the level of some vascular-inflammatory markers in the blood serum in patients with precancerous diseases and cervical cancer in order to improve their diagnosis and also to identify markers for predicting an unfavorable outcome in patients with cervical cancer.Materials and methods. A non-randomized prospective controlled study was carried out, the participants of which were patients with the diagnosis of cervical cancer (n = 49) and cervical intraepithelial neoplasia of the III degree (n = 13). The control group included 15 relatively healthy women. The following spectrum of inflammatory proteins was determined in blood serum by flow cytometry using the Human Vascular Inflammation Panel 1: myoglobin, calprotectin, lipocalin A, matrix metal peroxidase 2, osteopontin, myeloperoxidase, serum amyloid A, protein 4, which binds insulin-like growth factor cell-cell adhesion 1, vascular cell adhesion molecule, matrix metalloperoxidase 9, cystatin C. Statistical analysis was carried out by calculating the Mann-Whitney test with Bonferroni’s correction. The model was created using binary logistic regression to diagnose cervical cancer.Results. In the intergroup comparison of the protein`s spectrum in the blood serum, no significant differences were obtained. However, using the binary logistic regression method, an equation was drawn up to calculate the diagnostic coefficient of cervical cancer, which allows diagnosing cervical cancer with an accuracy of 82%, and in terms of information content is not inferior to cytological diagnostics. The developed coefficient can be used to predict an unfavorable outcome of cervical cancer after 1 year from the moment of diagnosis. Conclusion. The developed diagnostic coefficient makes it possible to  diagnose cervical cancer with high accuracy and can be used to predict cervical cancer

RECONSTRUCTION OF HYPOPHYSIAL-THYROID LINK OF REGULATION OF REPRODUCTIVE SYSTEM IN WOMEN WITH CHRONIC VIRAL HEPATITIS

The article presents the results of a study of levels of hormones of hypophysial-thyroid, system, in women with parenteral chronic viral hepatitis B and C in the light menstrua! disorders. We examined. 77 women of reproductive age: 42 patients with, chronic viral hepatitis and 35 healthy controls. We determined, the concentration of thyroid-stimulating hormone, triiodothyronine, thyroxine, free triiodothyronine and. free thyroxine. We identified. some differences in the concentrations of the studied, hormones in patients with, viral hepatitis depending on the nature of the menstrual cycle

HIV and pregnancy: realities of XXI century

The human immunodeficiency virus (HIV) continues to be a major global public health issue, having claimed 34 million lives so far. 38 million people are living with HIV around the world. 2 million of these people are children. The high medical, social and economic significance of HIV infection is determined by the widespread and severe course of the disease. Every year, up to 2.7 million new infections are registered worldwide. Up to 30 % of cases of HIV infection in pregnant women are detected for the first time during pregnancy-related examinations. Every year, 1.49 million babies are born to HIV-positive women worldwide. The current tasks are to preserve the health of women and reduce the risk of transmission of HIV infection from mother to child. The article analyzes the results of Russian and foreign studies concerning the problems of pregnant HIV-positive women, published in international databases. The aim of the work is to analyze the data of studies aimed at studying the effect of HIV infection on the course of pregnancy

State of lipid peroxidation - antioxidant system in men with diabetes mellitus type 1

The aim of study is to assess lipid peroxidation and antioxidant system in men with diabetes mellitus type 1. The objective of this study was to identify features of the lipid peroxidation-antioxidant defense system in men of reproductive age with diabetes mellitus type 1. 22 men with diabetes mellitus type 1 (the average age of 27.1 ± 7.38years) and30 healthy men of the same age (control group) (the average age of 28.0 ± 4.3 years) were involved in the study. The main and control groups were formed on the principle of "copy - pair". Standard clinical examination methods were used: history taking, physical examination and consultation of specialists to identify complications of diabetes. To determine the indicators of the system of free-radical lipid oxidation and activity of antioxidant protection, the spectrophotometric and fluorometric methods were used. In men with diabetes mellitus 1 type, there is increase in the level of active products reacting with thiobarbituric acid compared to the same indicator in the control group. The level of antioxidant protection in men with diabetes mellitus 1 type unlike the control was characterized by decrease in total antioxidant activity. However, stability of processes of superoxide dismutase activity and the glutathione system in comparison with control is observed against the backdrop of intensity increase of oxidative processes. Activation of lipid peroxidation is observed in men with diabetes mellitus type 1

DISTINCTIVE FEATURES OF HORMONAL STATUS IN MALE ADOLESCENTS WITH ESSENTIAL HYPERTENSION AND OBSTRUCTIVE SLEEP APNEA

Background. It is known that obstructive sleep apnea (OSA) has been closely linked to essential hypertension (EH) and is considered as an important stressor. The hypothalamic-pituitary-adrenal axis (HPA) is a major component involved in stress response. However, the response of HPA axis to OSA effects may be variable. Aim: to estimate hormonal status in OSA and non-OSA adolescents with EH. Methods. We examined 38 male adolescents with EH (18 OSA patients - group A, 20 non-OSA patients - group B) aged 14-17 years. OSA was verified by polysomnography applying system GRASS-TeLeFACTOR Twin PSG (Comet, USA). EH was diagnosed with 24-h ambulatory blood pressure monitoring using monitor Oscar 2 for OXFORD Medilog Prima. Hormonal status, including thyroid-stimulating hormone (TSH), prolactin (PRL) and cortisol, were routinely collected using an automatic analyzer «Cobos ELL» (USA) and test-system «Alcor-bio» (Russia). All differences were considered significant at p < 0.05. Results. There were statistically significant differences in morning serum cortisol level of OSA adolescents (745.8 ± 6.4 nmol/L versus 493.2 ± 3.2 nmol/L in non-OSA participants; р < 0.05). Morning serum PRL and TSH levels in the both groups no statistically significant differences, but there is a trend towards its increasing in OSA adolescents with EH (482.8 ± 4.2 mME/L in the group A and 442.2 ± 4.1 mME/L in the group B, and 1.95 ± 0.9 mkME/ml versus 1.5 ± 0.2 mkME/ml, respectively, р < 0.05). Conclusion. The results of this study indicate for chronic stress if OSA in adolescents with EH is present. This is due to the influence of sleep fragmentation and intermittent hypoxia on HPA axis with compensatory stress-related hormones release

Neuroendocrine regulation and ovarian reserve in HIV-infected women with reproductive disorders

Background: Among the subjects of the Russian Federation, Irkutsk region is an area with an unfavorable epidemiological situation for HIV-infection. Women living with HIV in Russia and visiting AIDS-centers represent a socially and economically active part of the population are at a young reproductive age and plan to have children. Aims: to study the state of the neuroendocrine system and the ovarian reserve in HIV-infected women with reproductive disorders. Materials and methods. We examined in 17 HIV-infected women with reproductive disorders and 33 women with HIV-infection and reproductive health at the age of 18-45 years (mean age 31,1 ± 4,4 years). The determination of hormone levels was carried out by a competitive solid-phase enzyme-linked immunosorbent assay. Results. The obtained results testify to neuroendocrine disorders in the form of a decrease in the level of total testosterone, 17-OH progesterone, dehydroepiandrosterone sulfate, as a manifestation of androgen deficiency of the body, as a result of ovarian dysfunction. Decrease in the level of Anti-Mullerian Hormone and increase the content of follicle-stimulating hormone, as a manifestation of the beginning of early menopause or a factor leading to infertility. Conclusions. The results of this study show that a woman's body on the background of HIV-infection reveals disorders of the neuroendocrine system dysfunction and decreased ovarian ovarian reserve, and as a result, reproductive disorders

THYROID FUNCTION AND REPRODUCTIVE HEALTH OF TEENAGE BOYS OF MAJOR ETHNIC GROUPS IN EASTERN SIBERIA

This paper presents the results of a retrospective analysis of epidemiological studies (2005-2011 years) continuous sampling method 320 adolescent boys aged 14-17 years living in the Irkutsk region (Irkutsk, Bratsk, Angarsk, v. Bay-anday): 282 boys Europeans, 30 Mongoloids, 8 Mitis. We compared the clinical, instrumental and laboratory data in adolescents, depending on where you live, the presence or absence of pathology and ethnic groups in the Russian, Buryat and Mitis. Ethnicity was determined by taking into account the phenotypic characteristics of the child and genealogical data history (children with parents in two generations of one ethnic group). The high frequency of goiter in teenage boys who live in the major industrial cities in the 2.63.4 times the figure given in the rural population (px2 0,05), living in rural areas, with no significant ethnic differences. However, the teenagers Caucasians living in urban areas, the puberty and physical development delay met in 2,5-1,4 times more often (33,8-58,3 %; px2 < 0,05). Our data support a role of thyroid dysfunction in the development of reproductive disorders, and therefore timely diagnosis, treatment and recovery of thyroid function is necessary for the prevention of infertility. The established differences in urban and rural adolescent boys suggest the influence of anthropogenic impact on the functioning of the endocrine and reproductive systems

Assessment of functional state of the pituitary-gonadal system in fertile women with acute hepatitis A

Liver is a necessary participant of the metabolism of steroid hormones, so dysfunction of pituitary-gonadal system is possible at viral hepatitis A. We assessed functional state of pituitary-gonadal part of neuroendocrinal regulation system in fertile women with acute hepatitis A and detected changes that can be regarded as compensatory-adaptive shielding of an organism

Trichology features of alopecia in reproductive age women with polycystic ovary syndrome

Background. Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders, affecting 5-10 % of reproductive-aged women. The dermatologic manifestations of PCOS play a significant role in diagnosis. The purpose of the present study was to examine the impact of androgenic alopecia among patients with PCOS and to define their most significant trkhological features. Because the data on dermatologic changes associated with PCOS are rather scarce, the present study was designed to determine the incidence and prevalence of androgenic alopecia. Materials and methods. We conducted a prospective observational study of patients with PCOS. The diagnosis of PCOS was made according to the consensus criteria adopted in May2003 in Rotterdam. Each patient signed informed consent, and then a general physical examination, systemic examination, breast and pelvic examination, as well as a detailed trichological examination were carried out. Results. Twenty women with PCOS participated in the study. The median age was28.7± 6.2years. Oligo/anovulation frequency was 63.2 %, hirsutism frequency - 68.4 %. In our study, the prevalence of androgenic alopecia was found to be 30 %, telogenic hair loss - 55 %, absence of alopecia signs in І5 % of cases. Conclusions. Androgenic alopecia is a prevalent but unreliable marker of biochemical hyperandrogenism. It is therefore imperative for the treating physicians to understand the adverse effects associated with PCOS, recognize these potential health risks in patients, and treat them accordingly

Features of the interrelationships of some molecular parameters of cervical epithelium cells with biological characteristics of tumor cells in the process of cervical carcinogenesis

Background. Despite the known trigger and identified risk factors, the screening program developed, many aspects of the pathogenesis of cervical cancer are still being studied. In particular, recently in the literature there are data on the participation of short-chain fatty acids in the tumor process. The aim of the study was to perform a correlation analysis of the level of TNF-A., its soluble sTNF-RI receptor, the spectrum of SCFA and some biological parameters (apoptosis, necrosis, proliferation, cell cycle) in the exocervical cells during dys- and neoplastic transformation. Materials and methods: the laboratory data obtained by us earlier were used to perform the Spearman correlation analysis. The groups of research: IA - the focus of the pre-tumor lesion of exocervix; IB - paradysplastic cells; IIA - locus of cervical cancer; IIB - paraneoplastic cells. Results. The correlation relationships between the parameters studied were multidirectional, determined by the state of the cell (healthy, dysplastic, malignant) and varied within one study, depending on the location in relation to the pathological focus. Conclusions. The revealed interrelations are important for expanding the already available information on cervical carcinogenesis, for discovery of new diagnostic methods and treatment of cervical neoplasia. Summarizing, it is possible to judge the presence of pathogenetic relationships between the processes of cervical carcinogenesis (proliferation, apoptosis, necrosis, modulation of the phases of the cell cycle), the level of short-chain fatty acids and cytokine production at the local level