Identification of the master sex determining gene in Northern pike (Esox lucius) reveals restricted sex chromosome differentiation.

Teleost fishes, thanks to their rapid evolution of sex determination mechanisms, provide remarkable opportunities to study the formation of sex chromosomes and the mechanisms driving the birth of new master sex determining (MSD) genes. However, the evolutionary interplay between the sex chromosomes and the MSD genes they harbor is rather unexplored. We characterized a male-specific duplicate of the anti-Müllerian hormone (amh) as the MSD gene in Northern Pike (Esox lucius), using genomic and expression evidence as well as by loss-of-function and gain-of-function experiments. Using RAD-Sequencing from a family panel, we identified Linkage Group (LG) 24 as the sex chromosome and positioned the sex locus in its sub-telomeric region. Furthermore, we demonstrated that this MSD originated from an ancient duplication of the autosomal amh gene, which was subsequently translocated to LG24. Using sex-specific pooled genome sequencing and a new male genome sequence assembled using Nanopore long reads, we also characterized the differentiation of the X and Y chromosomes, revealing a small male-specific insertion containing the MSD gene and a limited region with reduced recombination. Our study reveals an unexpectedly low level of differentiation between a pair of sex chromosomes harboring an old MSD gene in a wild teleost fish population, and highlights both the pivotal role of genes from the amh pathway in sex determination, as well as the importance of gene duplication as a mechanism driving the turnover of sex chromosomes in this clade

A model perspective on the dynamics of the shadow zone of the eastern tropical North Atlantic – Part 1: the poleward slope currents along West Africa

International audienceThe West African seaboard is one of the upwelling sectors that has received the least attention, and in situ observations relevant to its dynamics are particularly scarce. The current system in this sector is not well known and understood, e.g., in terms of seasonal variability, across-shore structure, and forcing processes. This knowledge gap is addressed in two studies that analyze the mean seasonal cycle of an eddy-permitting numerical simulation of the tropical Atlantic. Part 1 is concerned with the circulation over the West African continental slope at the southernmost reach of the Canary Current system, between  ∼ 8 and 20°N. The focus is on the depth range most directly implicated in the wind-driven circulation (offshore and coastal upwellings and Sverdrup transport) located above the potential density σt = 26.7 kg m−3 in the model (approx. above 250m of depth). In this sector and for this depth range, the flow is predominantly poleward as a direct consequence of positive wind stress curl forcing, but the degree to which the magnitude of the upper ocean poleward transport reflects Sverdrup theory varies with latitude. The model poleward flow also exhibits a marked semiannual cycle with transport maxima in spring and fall. Dynamical rationalizations of these characteristics are offered in terms of wind forcing of coastal trapped waves and Rossby wave dynamics. Remote forcing by seasonal fluctuations of coastal winds in the Gulf of Guinea plays an instrumental role in the fall intensification of the poleward flow. The spring intensification appears to be related to wind fluctuations taking place at shorter distances north of the Gulf of Guinea entrance and also locally. Rossby wave activity accompanying the semiannual fluctuations of the poleward flow in the coastal waveguide varies greatly with latitude, which in turn exerts a major influence on the vertical structure of the poleward flow. Although the realism of the model West African boundary currents is difficult to determine precisely, the present in-depth investigation provides a renewed framework for future observational programs in the region

Sex chromosome and sex locus characterization in goldfish, Carassius auratus (Linnaeus, 1758).

Goldfish is an important model for various areas of research, including neural development and behavior and a species of significant importance in aquaculture, especially as an ornamental species. It has a male heterogametic (XX/XY) sex determination system that relies on both genetic and environmental factors, with high temperatures being able to produce female-to-male sex reversal. Little, however, is currently known on the molecular basis of genetic sex determination in this important cyprinid model. Here we used sequencing approaches to better characterize sex determination and sex-chromosomes in an experimental strain of goldfish. Our results confirmed that sex determination in goldfish is a mix of environmental and genetic factors and that its sex determination system is male heterogametic (XX/XY). Using reduced representation (RAD-seq) and whole genome (pool-seq) approaches, we characterized sex-linked polymorphisms and developed male specific genetic markers. These male specific markers were used to distinguish sex-reversed XX neomales from XY males and to demonstrate that XX female-to-male sex reversal could even occur at a relatively low rearing temperature (18 °C), for which sex reversal has been previously shown to be close to zero. We also characterized a relatively large non-recombining region (~ 11.7 Mb) on goldfish linkage group 22 (LG22) that contained a high-density of male-biased genetic polymorphisms. This large LG22 region harbors 373 genes, including a single candidate as a potential master sex gene, i.e., the anti-Mullerian hormone gene (amh). However, no sex-linked polymorphisms were detected in the coding DNA sequence of the goldfish amh gene. These results show that our goldfish strain has a relatively large sex locus on LG22, which is likely the Y chromosome of this experimental population. The presence of a few XX males even at low temperature also suggests that other environmental factors in addition to temperature could trigger female-to-male sex reversal. Finally, we also developed sex-linked genetic markers, which will be important tools for future research on sex determination in our experimental goldfish population. However, additional work would be needed to explore whether this sex locus is conserved in other populations of goldfish

A numerical study of the circulation and monthly-to-seasonal variability in the Caribbean Sea: the role of Caribbean eddies

This study examines the circulation and associated monthly-to-seasonal variability in the Caribbean Sea using a regional ocean circulation model. The model domain covers the region between 99.0 and 54.0°W and between 8.0 and 30.3°N, with a horizontal resolution of 1/6°. The ocean circulation model is driven by 6-hourly atmospheric reanalysis data from the National Center for Environmental Prediction and boundary forcing extracted from 5-day global ocean reanalysis data produced by Smith et al. (Mercator Newsletter 36:39–49, 2010), and integrated for 7 years. A comparison of model results with observations demonstrates that the regional ocean circulation model has skill in simulating circulation and associated variability in the study region. Analysis of the model results, as well as a companion model run that uses steady annual mean forcing, illustrates the role of Caribbean eddies for driving monthly-to-seasonal circulation variability in the model. It is found that vertically integrated transport between Nicaragua and Jamaica is influenced by the interaction between the density perturbations associated with Caribbean eddies and the Nicaraguan Ridge. The impact of Caribbean eddies squeezing through the Yucatan Channel is also discussed

A supernumerary "B-sex" chromosome drives male sex determination in the Pachón cavefish, Astyanax mexicanus.

Sex chromosomes are generally derived from a pair of classical type-A chromosomes, and relatively few alternative models have been proposed up to now. <sup>1</sup> <sup>,</sup> <sup>2</sup> B chromosomes (Bs) are supernumerary and dispensable chromosomes with non-Mendelian inheritance found in many plant and animal species <sup>3</sup> <sup>,</sup> <sup>4</sup> that have often been considered as selfish genetic elements that behave as genome parasites. <sup>5</sup> <sup>,</sup> <sup>6</sup> The observation that in some species Bs can be either restricted or predominant in one sex <sup>7-14</sup> raised the interesting hypothesis that Bs could play a role in sex determination. <sup>15</sup> The characterization of putative B master sex-determining (MSD) genes, however, has not yet been provided to support this hypothesis. Here, in Astyanax mexicanus cavefish originating from Pachón cave, we show that Bs are strongly male predominant. Based on a high-quality genome assembly of a B-carrying male, we characterized the Pachón cavefish B sequence and found that it contains two duplicated loci of the putative MSD gene growth differentiation factor 6b (gdf6b). Supporting its role as an MSD gene, we found that the Pachón cavefish gdf6b gene is expressed specifically in differentiating male gonads, and that its knockout induces male-to-female sex reversal in B-carrying males. This demonstrates that gdf6b is necessary for triggering male sex determination in Pachón cavefish. Altogether these results bring multiple and independent lines of evidence supporting the conclusion that the Pachón cavefish B is a "B-sex" chromosome that contains duplicated copies of the gdf6b gene, which can promote male sex determination in this species

Sex determination diversity in fish

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