14 research outputs found
Metabolic profiling of human saliva before and after induced physiological stress by ultra-high performance liquid chromatography–ion mobility–mass spectrometry
This paper was accepted for publication in the journal, Metabolomics. The final publication is available at Springer via http://dx.doi.org/10.1007/s11306-013-0541-xA method has been developed for metabolite profiling of the salivary metabolome based on protein precipitation and ultra-high performance liquid chromatography coupled with ion mobility-mass spectrometry (UHPLC–IM–MS). The developed method requires 0.5 mL of human saliva, which is easily obtainable by passive drool. Standard protocols have been established for the collection, storage and pre-treatment of saliva. The use of UHPLC allows rapid global metabolic profiling for biomarker discovery with a cycle time of 15 min. Mass spectrometry imparts the ability to analyse a diverse number of species reproducibly over a wide dynamic range, which is essential for profiling of biofluids. The combination of UHPLC with IM–MS provides an added dimension enabling complex metabolic samples to be separated on the basis of retention time, ion mobility and mass-to-charge ratio in a single chromatographic run. The developed method has been applied to targeted metabolite identification and untargeted metabolite profiling of saliva samples collected before and after exercise-induced physiological stress. δ-Valerolactam has been identified as a potential biomarker on the basis of retention time, MS/MS spectrum and ion mobility drift time
Density Functional Theory Study of the Oxygen Reduction Reaction on a Cobalt–Polypyrrole Composite Catalyst
Anomalous enhancement of fluorescence of carbon dots through lanthanum doping and potential application in intracellular imaging of ferric ion
High-Resolution Hydrodynamic Chromatographic Separation of Large DNA Using Narrow, Bare Open Capillaries: A Rapid and Economical Alternative Technology to Pulsed-Field Gel Electrophoresis?
DFT Study of Polyaniline and Metal Composites as Nonprecious Metal Catalysts for Oxygen Reduction in Fuel Cells
Charge Tunable Zwitterionic Polyampholyte Layers Formed in Cyclic Olefin Copolymer Microchannels through Photochemical Graft Polymerization
Global urinary metabolic profiling procedures using gas chromatography-mass spectrometry
10.1038/nprot.2011.375Nature Protocols6101483-149