Measurements of Grain Motion in a Dense, Three-Dimensional Granular Fluid

We have used an NMR technique to measure the short-time, three-dimensional displacement of grains in a system of mustard seeds vibrated vertically at 15g. The technique averages over a time interval in which the grains move ballistically, giving a direct measurement of the granular temperature profile. The dense, lower portion of the sample is well described by a recent hydrodynamic theory for inelastic hard spheres. Near the free upper surface the mean free path is longer than the particle diameter and the hydrodynamic description fails.Comment: 4 pages, 4 figure

Grey GERT Network Model of Equipment Lifetime Evaluation Based on Small Samples

The reliability evaluation of high reliability and long life equipment is widely concerned in recent decades. Enough failure samples of these kinds of equipment are not easy or economic to obtain in reliability test, in addition, experience information is sometimes inaccurate or uncertainty. To overcome the deficiency in traditional method which requires large numbers of samples, a quantitative analysis model of equipment reliability evaluation is proposed in this paper in view of the few failure data of equipment life tests. GERT network is introduced to describe the kinds of working states of the equipment system and random process of equipment state transition choice after stress impact of single component. Considering the uncertainty and inaccuracy of the statistical data and experience information, the parameters of GERT network are represented by interval grey number. The system equivalent transfer function could be obtained by GERT matrix solving algorithm, and the reliability evaluation of equipment system can be realized. The case study results show that the equipment reliability evaluation Grey-GERT model based on small samples would save much time with little accuracy losing. Besides, the study provides a new thinking for reliability accelerated life test

A Warning System for Anthracnose Fruit Rot on Strawberries

Anthracnose fruit rot (AFR) of strawberry is caused by three Colletotrichum spp. In the Midwest, however, only Colletotrichum acutatum is found. This fungus can attach itself to apparently healthy plants and spread throughout the field without causing symptoms on the foliage. When fruit beginsto ripen and weather conditions are rainy and warm, AFR can suddenly cause great damage to the fruit. To protect against AFR where it has appeared in the past, growers need to spray every 7 to 10 days beginning at the start of bloom until harvest

The temporal changes in the emission spectrum of Comet 9P/Tempel 1 after Deep Impact

The time dependence of the changes in the emission spectra of Comet 9P/Tempel 1 after Deep Impact are derived and discussed. This was a unique event because for the first time it gave astronomers the opportunity to follow the time history of the formation and decay of O(1S), OH, CN, C2, C3, NH, and NH2. Least squares fits of a modified Haser model with constraints using known rate constants were fit to the observed data. In the case of OH a simple two-step Haser model provides a reasonable fit to the observations. Fitting the emissions from O(1S), CN, C2, C3, NH, and NH2 requires the addition of a delayed component to a regular two or three step Haser model. From this information a picture of the Deep Impact encounter emerges where there is an initial formation of gas and dust, which is responsible for the prompt emission that occurs right after impact. A secondary source of gas starts later after impact when the initial dust has dissipated enough so that solar radiation can reach the surface of freshly exposed material. The implications of this and other results are discussed in terms of the implications on the structure and composition of the comet's nucleus.Comment: Accepted for publication in The Astrophysical Journal; 26 pages including 8 figures and 1 tabl

Testing a Warning System for Anthracnose Fruit Rot on Day-neutral Strawberry

Anthracnose fruit rot (AFR) of strawberry is caused by three Colletotrichum spp. In the Midwest, however, only Colletotrichum acutatum is found. This fungus can attach itself to apparently healthy plants and spread throughout without causing symptoms on the foliage. When fruit begin to ripen and weather conditions are rainy and warm, AFR can suddenly cause great damage to the fruit. To protect against AFR where it has appeared in the past, growers need to spray every 7 to 10 days beginning at the start of bloom until harvest

Dual Role of a Viral Polymerase in Viral Genome Replication and Particle Self-Assembly

Double-stranded RNA (dsRNA) viruses package several RNA-dependent RNA polymerases (RdRp) together with their dsRNA genome into an icosahedral protein capsid known as the polymerase complex. This structure is highly conserved among dsRNA viruses but is not found in any other virus group. RdRp subunits typically interact directly with the main capsid proteins, close to the 5-fold symmetric axes, and perform viral genome replication and transcription within the icosahedral protein shell. In this study, we utilized Pseudomonas phage Phi 6, a well-established virus self-assembly model, to probe the potential roles of the RdRp in dsRNA virus assembly. We demonstrated that Phi 6 RdRp accelerates the polymerase complex self-assembly process and contributes to its conformational stability and integrity. We highlight the role of specific amino acid residues on the surface of the RdRp in its incorporation during the self-assembly reaction. Substitutions of these residues reduce RdRp incorporation into the polymerase complex during the self-assembly reaction. Furthermore, we determined that the overall transcription efficiency of the Phi 6 polymerase complex increased when the number of RdRp subunits exceeded the number of genome segments. These results suggest a mechanism for RdRp recruitment in the polymerase complex and highlight its novel role in virion assembly, in addition to the canonical RNA transcription and replication functions. IMPORTANCE Double-stranded RNA viruses infect a wide spectrum of hosts, including animals, plants, fungi, and bacteria. Yet genome replication mechanisms of these viruses are conserved. During the infection cycle, a proteinaceous capsid, the polymerase complex, is formed. An essential component of this capsid is the viral RNA polymerase that replicates and transcribes the enclosed viral genome. The polymerase complex structure is well characterized for many double-stranded RNA viruses. However, much less is known about the hierarchical molecular interactions that take place in building up such complexes. Using the bacteriophage Phi 6 self-assembly system, we obtained novel insights into the processes that mediate polymerase subunit incorporation into the polymerase complex for generation of functional structures. The results presented pave the way for the exploitation and engineering of viral self-assembly processes for biomedical and synthetic biology applications. An understanding of viral assembly processes at the molecular level may also facilitate the development of antivirals that target viral capsid assembly.Peer reviewe

Solvent Effects on Extractant Conformational Energetics in Liquid-Liquid Extraction: A Simulation Study of Molecular Solvents and Ionic Liquids

Extractant design in liquid-liquid extraction (LLE) is a research frontier of metal ion separations that typically focuses on the direct extractant-metal interactions. However, a more detailed understanding of energetic drivers of separations beyond primary metal coordination is often lacking, including the role of solvent in the extractant phase. In this work, we propose a new mechanism for enhancing metal-complexant energetics with nanostructured solvents. Using molecular dynamics simulations with umbrella sampling, we find that the organic solvent can reshape the energetics of the extractant's intramolecular conformational landscape. We calculate free energy profiles of different conformations of a representative bidentate extractant, n-octyl(phenyl)-N,N-diisobutyl carbamoyl methyl phosphinoxide (CMPO), in four different solvents: dodecane, tributyl phosphate (TBP), and dry and wet ionic liquid (IL) 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([EMIM][Tf_2N]). By promoting reorganization of the extractant molecule into its binding conformation, our findings reveal how particular solvents can ameliorate this unfavorable step of the metal separation process. In particular, the charge alternating nanodomains formed in ILs substantially reduce the free energy penalty associated with extractant reorganization. Importantly, using alchemical free energy calculations, we find that this stabilization persists even when we explicitly include the extracted cation. These findings provide insight into the energic drivers of metal ion separations and potentially suggest a new approach to designing effective separations using a molecular-level understanding of solvent effects

Gene Structure Induced Epigenetic Modifications of pericarp color1 Alleles of Maize Result in Tissue-Specific Mosaicism

BACKGROUND: The pericarp color1 (p1) gene encodes for a myb-homologous protein that regulates the biosynthesis of brick-red flavonoid pigments called phlobahpenes. The pattern of pigmentation on the pericarp and cob glumes depends upon the allelic constitution at the p1 locus. p1 alleles have unique gene structure and copy number which have been proposed to influence the epigenetic regulation of tissue-specific gene expression. For example, the presence of tandem-repeats has been correlated with the suppression of pericarp pigmentation though a mechanism associated with increased DNA methylation. METHODOLOGY/PRINCIPAL FINDINGS: Herein, we extensively characterize a p1 allele called P1-mosaic (P1-mm) that has mosaic pericarp and light pink or colorless cob glumes pigmentation. Relative to the P1-wr (white pericarp and red cob glumes), we show that the tandem repeats of P1-mm have a modified gene structure containing a reduced number of repeats. The P1-mm has reduced DNA methylation at a distal enhancer and elevated DNA methylation downstream of the transcription start site. CONCLUSIONS/SIGNIFICANCE: Mosaic gene expression occurs in many eukaryotes. Herein we use maize p1 gene as model system to provide further insight about the mechanisms that govern expression mosaicism. We suggest that the gene structure of P1-mm is modified in some of its tandem gene repeats. It is known that repeated genes are susceptible to chromatin-mediated regulation of gene expression. We discuss how the modification to the tandem repeats of P1-mm may have disrupted the epigenetic mechanisms that stably confer tissue-specific expression

Exploring Entrepreneurial Roles and Identity in the United Kingdom and China

This paper examines entrepreneurial identity in both the United Kingdom and China through the lenses of identity theory and social identity theory to develop a deeper and more holistic understanding of the concept of entrepreneurial identity. By examining the entrepreneur as both a role and an identity this paper explores how an entrepreneur views the role of the entrepreneur, the counter-roles to the entrepreneur, the ‘self-as-entrepreneur’ understand how entrepreneurs construct their identity as entrepreneur. By looking at the role identity in different social constructs, a more nuanced view of entrepreneurial identity can be uncovered for entrepreneurs in both the UK and China. The study argues that entrepreneurs in the UK use counter-roles to bridge the disconnect between their understanding of the entrepreneur-as-role and the self-as-entrepreneur whereas entrepreneurs in China have less conflict reconciling the two, and use the counter-role as a way to paint entrepreneurship as a ‘calling’, justifying their abandonment of other identities