14 research outputs found
Observations on the biology and ecology of the chrysomelid beetle Gastrophysa polygoni in cereal fields
No full textZur Lebensweise und Wirtsbindung des Distel-BlattkäfersLema cyanella L.(puncticollis Curt.) (Col. Chrysomelidae)
No full textContributions to the chemistry of human hair: III. Protein chemical aspects of permanent waving treatments
No full textReactor Concept for Lipase-Catalyzed Solvent-Free Conversion of Highly Viscous Reactants Forming Two-Phase Systems
No full textReaction and strain engineering for improved stereo-selective whole-cell reduction of a bicyclic diketone
No full textReduction of bicyclo[2.2.2]octane-2,6-dione to (1R, 4S, 6S)-6-hydroxy-bicyclo[2.2.2]octane-2-one by whole cells of Saccharomyces cerevisiae was improved using an engineered recombinant strain and process design. The substrate inhibition followed a Han-Levenspiel model showing an effective concentration window between 12 and 22 g/l, in which the activity was kept above 95%. Yeast growth stage, substrate concentration and a stable pH were shown to be important parameters for effective conversion. The over-expression of the reductase gene YDR368w significantly improved diastereoselectivity compared to previously reported results. Using strain TMB4110 expressing YDR368w in batch reduction with pH control, complete conversion of 40 g/l (290 mM) substrate was achieved with 97% diastereomeric excess (de) and >99 enantiomeric excess (ee), allowing isolation of the optically pure ketoalcohol in 84% yield
Efficient production of diltiazem chiral intermediate using immobilized lipase from Serratia marcescens
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Enzymatic- and Iridium-Catalyzed Asymmetric Synthesis of a Benzothiazepinylphosphonate Bile Acid Transporter Inhibitor
No full textLarval parasites ofOulema melanopus in Europe and their colonization in the United States
No full textReversible immobilization of Candida rugosa lipase on fibrous polymer grafted and sulfonated p(HEMA/EGDMA) beads
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