220 research outputs found
Genetic differentiation of Anopheles gambiae populations from East and West Africa : comparison of microsatellite and allozyme loci
Genetic variation of #Anopheles gambiae$ was analysed to assess interpopulation divergence over a 6000 km distance using short tandem repeat (microsatellite) loci and allozyme loci. Differentiation of populations from Kenya and Senegal measured by allele length variation at five microsatellite loci was compared with estimates calculated from published data on six allozyme loci (Miles, 1978). The average Wright's F(ST) of microsatellite loci (0.016) was lower than that of allozymes (0.036). Slatkin's R(ST) values for microsatellite loci were generally higher than their F(ST) values, but the average R(ST) value was virtually identical (0.036) to the average allozyme F(ST). These low estimates of differentiation correspond to an effective migration index (Nm) larger than 3, suggesting that gene flow across the continent is only weakly restricted. Polymorphism of microsatellite loci was significantly higher than that of allozymes, probably because the former experience considerably higher mutation rates. That microsatellite loci did not measure greater interpopulation divergence than allozyme loci suggested constraints on microsatellite evolution. Alternatively, extensive mosquito dispersal, aided by human transportation during the last century, better explains the low differentiation and the similarity of estimates derived from both types of genetic markers. (Résumé d'auteur
Establishment and characterization of three cell lines from Aedes triseriatus (Diptera : Culicidae)
Program and abstracts of the 44th annual meeting of the american society of tropical medicine and hygiene
Comparaison de la transmission du paludisme dans deux faciès épidémiologiques au Sénégal: la zone côtière sahélienne et la zone méridionale soudanienne
Aménagements hydro-agricoles et santé (vallée du fleuve Sénégal)
La transmission du paludisme dans le delta du fleuve Sénégal a été étudiée dans trois villages, deux villages de riziculture irriguée dont un d'aménagement récent et un village de culture pluviale traditionnelle. La riziculture irriguée a entraîné une pullulation de moustiques et principalement d'#Anopheles pharoensis. La densité de ses populations est très importante et liée aux cycles des cultures dans la zone rizicole, son indice d'anthropophilie est élevé mais la longévité des femelles est très faible. #An. gambiae s.l. vecteur majeur du paludisme au Sénégal est, dans le delta, remplacé par #An. pharoensis$, vecteur secondaire dont l'aptitude à assurer une bonne transmission n'est pas prouvée. La riziculture irriguée n'a pas entraîné une augmentation de la transmission du paludisme. (Résumé d'auteur
Susceptibility of Anopheles stephensi to Plasmodium gallinaceum: A Trait of the Mosquito, the Parasite, and the Environment
Vector susceptibility to Plasmodium infection is treated primarily as a vector trait, although it is a composite trait expressing the joint occurrence of the parasite and the vector with genetic contributions of both. A comprehensive approach to assess the specific contribution of genetic and environmental variation on "vector susceptibility" is lacking. Here we developed and implemented a simple scheme to assess the specific contributions of the vector, the parasite, and the environment to "vector susceptibility." To the best of our knowledge this is the first study that employs such an approach.We conducted selection experiments on the vector (while holding the parasite "constant") and on the parasite (while holding the vector "constant") to estimate the genetic contributions of the mosquito and the parasite to the susceptibility of Anopheles stephensi to Plasmodium gallinaceum. We separately estimated the realized heritability of (i) susceptibility to parasite infection by the mosquito vector and (ii) parasite compatibility (transmissibility) with the vector while controlling the other. The heritabilities of vector and the parasite were higher for the prevalence, i.e., fraction of infected mosquitoes, than the corresponding heritabilities of parasite load, i.e., the number of oocysts per mosquito.The vector's genetics (heritability) comprised 67% of "vector susceptibility" measured by the prevalence of mosquitoes infected with P. gallinaceum oocysts, whereas the specific contribution of parasite genetics (heritability) to this trait was only 5%. Our parasite source might possess minimal genetic diversity, which could explain its low heritability (and the high value of the vector). Notably, the environment contributed 28%. These estimates are relevant only to the particular system under study, but this experimental design could be useful for other parasite-host systems. The prospects and limitations of the genetic manipulation of vector populations to render the vector resistant to the parasite are better considered on the basis of this framework
Variations saisonnières des populations d'Anopheles gambiae s.l. et transmission du paludisme dans un village de savane soudanienne du Sud-Est du Sénégal
Using the entomological inoculation rate to assess the impact of vector control on malaria parasite transmission and elimination
Prior studies have shown that annual entomological inoculation rates (EIRs) must be reduced to less than one to substantially reduce the prevalence of malaria infection. In this study, EIR values were used to quantify the impact of insecticide-treated bed nets (ITNs), indoor residual spraying (IRS), and source reduction (SR) on malaria transmission. The analysis of EIR was extended through determining whether available vector control tools can ultimately eradicate malaria. The analysis is based primarily on a review of all controlled studies that used ITN, IRS, and/or SR and reported their effects on the EIR. To compare EIRs between studies, the percent difference in EIR between the intervention and control groups was calculated. Eight vector control intervention studies that measured EIR were found: four ITN studies, one IRS study, one SR study, and two studies with separate ITN and IRS intervention groups. In both the Tanzania study and the Solomon Islands study, one community received ITNs and one received IRS. In the second year of the Tanzania study, EIR was 90% lower in the ITN community and 93% lower in the IRS community, relative to the community without intervention; the ITN and IRS effects were not significantly different. In contrast, in the Solomon Islands study, EIR was 94% lower in the ITN community and 56% lower in the IRS community. The one SR study, in Dar es Salaam, reported a lower EIR reduction (47%) than the ITN and IRS studies. All of these vector control interventions reduced EIR, but none reduced it to zero. These studies indicate that current vector control methods alone cannot ultimately eradicate malaria because no intervention sustained an annual EIR less than one. While researchers develop new tools, integrated vector management may make the greatest impact on malaria transmission. There are many gaps in the entomological malaria literature and recommendations for future research are provided
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