37 research outputs found
A single residue substitution in the receptor-binding domain of H5N1 hemagglutinin is critical for packaging into pseudotyped lentiviral particles
- Author
- AM Kilpatrick
- AN Abdel-Ghafar
- B Labrosse
- Béatrice Nal
- C Tsai
- Chi-Hong Lam
- DA Senne
- DA Steinhauer
- Dong-Jiang Tang
- DP Nayak
- G Kayali
- GM Air
- H Chen
- H Imai
- HD Klenk
- I Nefkens
- J Szecsi
- J. S. Malik Peiris
- JJ Skehel
- JM Garcia
- JM Garcia
- JM Moore
- JS Peiris
- L Houzet
- M Lakadamyali
- M Matrosovich
- M Naughtin
- MR Sandbulte
- P Auewarakul
- P Benaroch
- Philippe Buchy
- RG Webster
- Roberto Bruzzone
- RP de Vries
- S Barman
- S Yamada
- Shui-Ling Chu
- SL Deutscher
- Stefan Pöhlmann
- SY Wang
- T Dung Nuyen
- T Horimoto
- T McKay
- T Rowe
- V Bosch
- VC Chu
- Y Suzuki
- Yu-Lam Siu
- Yuen-Man Lam
- Z Ao
- Publication venue
- 'Public Library of Science (PLoS)'
- Publication date
- 01/01/2012
- Field of study
© 2012 Tang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Background: Serological studies for influenza infection and vaccine response often involve microneutralization and hemagglutination inhibition assays to evaluate neutralizing antibodies against human and avian influenza viruses, including H5N1. We have previously characterized lentiviral particles pseudotyped with H5-HA (H5pp) and validated an H5pp-based assay as a safe alternative for high-throughput serological studies in BSL-2 facilities. Here we show that H5-HAs from different clades do not always give rise to efficient production of H5pp and the underlying mechanisms are addressed.
Methodology/Findings: We have carried out mutational analysis to delineate the molecular determinants responsible for efficient packaging of HA from A/Cambodia/40808/2005 (H5Cam) and A/Anhui/1/2005 (H5Anh) into H5pp. Our results demonstrate that a single A134V mutation in the 130-loop of the receptor binding domain is sufficient to render H5Anh the ability to generate H5Anh-pp efficiently, whereas the reverse V134A mutation greatly hampers production of H5Cam-pp. Although protein expression in total cell lysates is similar for H5Anh and H5Cam, cell surface expression of H5Cam is detected at a significantly higher level than that of H5Anh. We further demonstrate by several independent lines of evidence that the behaviour of H5Anh can be explained by a stronger binding to sialic acid receptors implicating residue 134.
Conclusions: We have identified a single A134V mutation as the molecular determinant in H5-HA for efficient incorporation into H5pp envelope and delineated the underlying mechanism. The reduced binding to sialic acid receptors as a result of the A134V mutation not only exerts a critical influence in pseudotyping efficiency of H5-HA, but has also an impact at the whole virus level. Because A134V substitution has been reported as a naturally occurring mutation in human host, our results may have implications for the understanding of human host adaptation of avian influenza H5N1 virusesThis work was supported by grants from the Research Fund for the Control of Infectious Diseases of Hong Kong (RFCID#08070972), the Area of
Excellence Scheme of the University Grants Committee (grant AoE/M-12/-06 of the Hong Kong Special Administrative Region, China), the French Ministry of Health, and the RESPARI project of the Institut Pasteur International Network
Choline Dehydrogenase Polymorphism rs12676 Is a Functional Variation and Is Associated with Changes in Human Sperm Cell Function
- Author
- A Ferlin
- A Garcia-Perez
- AC Nevo
- AE Favier
- AM van Pelt
- Amy R. Johnson
- AR Johnson
- AS Prasad
- AV McGrady
- BT Storey
- BW Brown
- BW Geer
- C Foresta
- C Mukai
- C-H Yeung
- C-H Yeung
- CY Hong
- D Le Rudulier
- DM de Kretser
- DR Haubrich
- E Goldberg
- E Koskinen
- EM Eddy
- FS Steven
- H Koc
- HH Sandstead
- J de Ridder
- JK Thakkar
- Joseph A. Galanko
- K Miki
- KA da Costa
- L Chi-Shui
- L Huacuja
- LG Sanchez-Partida
- LR Fraser
- MA Baker
- MG O'Rand
- MK Chern
- N Garrido
- Neil John Gemmell
- PH Yancey
- PJG Mann
- PV Danshina
- R Eliasson
- RK Porter
- RK Porter
- RM Tombes
- RP Steegers-Theunissen
- S Huang
- SA Craig
- Sai Lao
- SC Sikka
- SH Zeisel
- SH Zeisel
- SH Zeisel
- SS Suarez
- Steven H. Zeisel
- TG Cooper
- TG Cooper
- TJ Porter
- Tongwen Wang
- U Kvist
- U Kvist
- V Michel
- WV Holt
- X Xu
- XJ Ma
- Z Wang
- Publication venue
- Public Library of Science
- Publication date
- 01/01/2011
- Field of study
Approximately 15% of couples are affected by infertility and up to half of these cases arise from male factor infertility. Unidentified genetic aberrations such as chromosomal deletions, translocations and single nucleotide polymorphisms (SNPs) may be the underlying cause of many cases of idiopathic male infertility. Deletion of the choline dehydrogenase (Chdh) gene in mice results in decreased male fertility due to diminished sperm motility; sperm from Chdh−/− males have decreased ATP concentrations likely stemming from abnormal sperm mitochondrial morphology and function in these cells. Several SNPs have been identified in the human CHDH gene that may result in altered CHDH enzymatic activity. rs12676 (G233T), a non-synonymous SNP located in the CHDH coding region, is associated with increased susceptibility to dietary choline deficiency and risk of breast cancer. We now report evidence that this SNP is also associated with altered sperm motility patterns and dysmorphic mitochondrial structure in sperm. Sperm produced by men who are GT or TT for rs12676 have 40% and 73% lower ATP concentrations, respectively, in their sperm. rs12676 is associated with decreased CHDH protein in sperm and hepatocytes. A second SNP located in the coding region of IL17BR, rs1025689, is linked to altered sperm motility characteristics and changes in choline metabolite concentrations in sperm
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
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- Abdel-Aziz A. K.
- Abdelfatah S.
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- Acevedo-Arozena A.
- Adamopoulos I. E.
- Adeli K.
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- Al-Gharaibeh A.
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- Alberti S.
- Alcocer-Gomez E.
- Alessandri C.
- Ali M.
- Alim Al-Bari M. A.
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- Zuryn S.
- Zwerschke W.
- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.
- Author
- Abdel-Aziz AK
- Abdelfatah S
- Abdellatif M
- Abdoli A
- Abel S
- Abeliovich H
- Abildgaard MH
- Abudu YP
- Acevedo-Arozena A
- Adamopoulos IE
- Adeli K
- Adolph TE
- Adornetto A
- Aflaki E
- Agam G
- Agarwal A
- Aggarwal BB
- Agnello M
- Agostinis P
- Agrewala JN
- Agrotis A
- Aguilar PV
- Ahmad ST
- Ahmed ZM
- Ahumada-Castro U
- Aits S
- Aizawa S
- Akkoc Y
- Akoumianaki T
- Akpinar HA
- Al-Abd AM
- Al-Akra L
- Al-Gharaibeh A
- Alaoui-Jamali MA
- Alberti S
- Alcocer-Gómez E
- Alessandri C
- Ali M
- Alim Al-Bari MA
- Aliwaini S
- Alizadeh J
- Almacellas E
- Almasan A
- Alonso A
- Alonso GD
- Altan-Bonnet N
- Altieri DC
- Alves da Costa C
- Alves S
- Alzaharna MM
- Amadio M
- Amantini C
- Amaral C
- Ambrosio S
- Amer AO
- Ammanathan V
- An Z
- Andersen SU
- Andrabi SA
- Andrade-Silva M
- Andres AM
- Angelini S
- Ann D
- Anozie UC
- Ansari MY
- Antas P
- Antebi A
- Antón Z
- Anwar T
- Apetoh L
- Apostolova N
- Araki T
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- Arasaki K
- Araya J
- Araújo WL
- Arden C
- Arguelles S
- Arias E
- Arikkath J
- Arimoto H
- Ariosa AR
- Armstrong-James D
- Arnauné-Pelloquin L
- Aroca A
- Arroyo DS
- Arsov I
- Artero R
- Arévalo M-A
- Asaro DML
- Aschner M
- Ashrafizadeh M
- Ashur-Fabian O
- Atanasov AG
- Au AK
- Auberger P
- Auner HW
- Aurelian L
- Autelli R
- Avagliano L
- Aveic S
- Aveleira CA
- Avin-Wittenberg T
- Aydin Y
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- Ayyadevara S
- Azzopardi M
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- Backues SK
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- Baksi S
- Balazadeh S
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- Baldari CT
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- Chang H-C
- Chang K
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- Charlet-Berguerand N
- Chatterjee S
- Chaube SK
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- Chaum E
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- Cheetham ME
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- Chen R-H
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- Chen W
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- Chen X
- Chen X-M
- Chen X-W
- Chen Y
- Chen Y
- Chen Y
- Chen Y-G
- Chen Y-J
- Chen Y-Q
- Chen Z
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- Chen Z
- Chen Z-H
- Chen ZJ
- Chen ZS
- Cheng H
- Cheng J
- Cheng S-Y
- Cheng W
- Cheng X
- Cheng X-T
- Cheng Y
- Cheng Z
- Cheong H
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- Chernyak BV
- Cherry S
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- Cheung CHA
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- Chiaradonna F
- Chiarelli R
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- Cianfanelli V
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- Cifuentes M
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- Consiglio A
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- Cordes N
- Corkery DP
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- Crack PJ
- Crespo JL
- Criollo A
- Crippa V
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- Csizmadia T
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- Czaja MJ
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- de Mattos Barbosa MG
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- Demarchi F
- Dembitz V
- Demers ND
- Deng H
- Deng Z
- Dengjel J
- Dent P
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- Der CJ
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- Devkota S
- Devuyst O
- Dewson G
- Dharmasivam M
- Dhiman R
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- Diallinas G
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- Dikic I
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- Distler JHW
- Diwan A
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- Dobrinski I
- Dobrovinskaya O
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- du Toit A
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- Díaz-Laviada I
- Ebrahimi-Fakhari D
- Eckhart L
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- Yi-Ren Hong C-WH
- Yin X-M
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- Yoo YH
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- Yousefi B
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- Yung RL
- Zacks DN
- Zaffagnini G
- Zambelli VO
- Zanella I
- Zang QS
- Zanivan S
- Zappavigna S
- Zaragoza P
- Zarbalis KS
- Zarebkohan A
- Zarrouk A
- Zeitlin SO
- Zeng J
- Zeng J-D
- Zhan L
- Zhang B
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- Zhang H-L
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- Zhang J-P
- Zhang KYB
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- Zhang X
- Zhang X
- Zhang X
- Zhang X
- Zhang X
- Zhang X-W
- Zhang XD
- Zhang Y
- Zhang Y
- Zhang Y
- Zhang Y
- Zhang Y
- Zhang Y-D
- Zhang Y-Y
- Zhang Z
- Zhang Z
- Zhang Z
- Zhang Z
- Zhang Z
- Zhang Z
- Zhao H
- Zhao L
- Zhao S
- Zhao T
- Zhao X-F
- Zhao Y
- Zhao Y
- Zhao Y
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- Zheng G
- Zheng K
- Zheng L
- Zheng S
- Zheng X-L
- Zheng Y
- Zheng Z-G
- Zhivotovsky B
- Zhong Q
- Zhou A
- Zhou B
- Zhou C
- Zhou G
- Zhou H
- Zhou H
- Zhou H
- Zhou J
- Zhou J
- Zhou J
- Zhou J
- Zhou K
- Zhou R
- Zhou X-J
- Zhou Y
- Zhou Y
- Zhou Y
- Zhou Z
- Zhou Z-Y
- Zhu B
- Zhu C
- Zhu G-Q
- Zhu H
- Zhu H
- Zhu H
- Zhu W-G
- Zhu Y
- Zhu Y
- Zhuang H
- Zhuang X
- Zientara-Rytter K
- Zimmermann CM
- Ziviani E
- Zoladek T
- Zong W-X
- Zorov DB
- Zorzano A
- Zou W
- Zou Z
- Zou Z
- Zuryn S
- Zwerschke W
- Álvarez ÉMC
- Ávalos Y
- Önal G
- Üstün S
- Čolić M
- Đokić J
- Žerovnik E
- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2021
- Field of study
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field
Non-Standard Errors
- Author
- Abad-Díaz David
- Abudy Menachem
- Adrian Tobias
- Ait-Sahalia Yacine
- Akmansoy Olivier
- Alcock Jamie T.
- Alexeev Vitali
- Aloosh Arash
- Amato Livia
- Amaya Diego
- Angel James J.
- Avetikian Alejandro T.
- Bach Amadeus
- Baidoo Edwin
- Bakalli Gaetan
- Bao Li
- Barbon Andrea
- Bashchenko Oksana
- Bindra Parampreet C.
- Bjønnes Geir H.
- Black Bernard S.
- Black Jeffrey R.
- Bogoev Dimitar
- Bohorquez Correa Santiago
- Bondarenko Oleg
- Bos Charles S.
- Bosch-Rosa Ciril
- Bouri Elie
- Brownlees Christian
- Calamia Anna
- Cao Viet Nga
- Capelle-Blancard Gunther
- Capera Romero Laura M.
- Caporin Massimiliano
- Carrion Allen
- Caskurlu Tolga
- Chakrabarty Bidisha
- Chen Jian
- Chernov Mikhail
- Cheung William
- Chincarini Ludwig B.
- Chordia Tarun
- Chow Sheung-Chi
- Clapham Benjamin
- Colliard Jean-Edouard
- Comerton-Forde Carole
- Curran Edward
- Dao Thong
- Dare Wale
- Davies Ryan J.
- De Blasis Riccardo
- De Nard Gianluca F.
- Declerck Fany
- Deev Oleg
- Degryse Hans
- Deku Solomon Y.
- Desagre Christophe
- Dim Chukwuma
- Dimpfl Thomas
- Dong Yun Jiang
- Dreber Anna
- Drummond Philip A.
- Dudda Tom
- Duevski Teodor
- Dumitrescu Ariadna
- Dyakov Teodor
- Dyhrberg Anne Haubo
- Dzielinski Michał
- Eksi Asli
- El Kalak Izidin
- Eugster Nicolas
- Evans Martin D. D.
- Farrell Michael
- Felez-Vinas Ester
- Ferrara Gerardo
- Ferrouhi El Mehdi
- Flori Andrea
- Fluharty Jonathan T.
- Foley Sean D. V.
- Fong Kingsley Y. L.
- Foucault Thierry
- Franus Tatiana
- Franzoni Francesco
- Frijns Bart
- Frömmel Michael
- Fu Servanna M.
- Füllbrunn Sascha C.
- Gan Baoqing
- Gao Ge
- Gehrig Thomas P.
- Gemayel Roland
- Gerritsen Dirk
- Gil-Bazo Javier
- Gilder Dudley
- Glosten Lawrence R.
- Gomez Thomas
- Gorbenko Arseny
- Grammig Joachim
- Grégoire Vincent
- Güçbilmez Ufuk
- Hagströmer Björn
- Hambuckers Julien
- Hapnes Erik
- Harris Jeffrey H.
- Harris Lawrence
- Hartmann Simon
- Hasse Jean-Baptiste
- Hautsch Nikolaus
- He Xue-Zhong (Tony)
- Heath Davidson
- Hediger Simon
- Hendershott Terrence
- Hibbert Ann Marie
- Hjalmarsson Erik
- Hoelscher Seth
- Hoffmann Peter
- Holden Craig W.
- Holzmeister Felix
- Horenstein Alex R.
- Huang Da
- Huang Wenqian
- Huber Juergen
- Hurlin Christophe
- Ilczuk Konrad
- Ivashchenko Alexey
- Iyer Subramanian R.
- Jahanshahloo Hossein
- Jalkh Naji P.
- Johannesson Magnus
- Jones Charles M.
- Jurkatis Simon
- Jylhä Petri
- Kaeck Andreas T.
- Kaiser Gabriel
- Karam Arzé
- Karmaziene Egle
- Kassner Bernhard
- Kaustia Markku
- Kazak Ekaterina
- Kearney Fearghal
- Kervel Vincent van
- Khan Saad A.
- Khomyn Marta K.
- Kirchler Michael
- Klein Olga
- Klein Tony
- Klos Alexander
- Koetter Michael
- Kolokolov Aleksey
- Korajczyk Robert A.
- Kozhan Roman
- Krahnen Jan P.
- Kuhle Paul
- Kwan Amy
- Lajaunie Quentin
- Lam F. Y. Eric C.
- Lambert Marie
- Langlois Hugues
- Lausen Jens
- Lauter Tobias
- Leippold Markus
- Levin Vladimir
- Li Hui
- Li Yijie
- Liew Chee Yoong
- Lindner Thomas
- Linton Oliver
- Liu Anqi
- Liu Jiacheng
- Llorente Guillermo
- Lof Matthijs
- Lohr Ariel
- Longstaff Francis
- Lopez-Lira Alejandro
- Mankad Shawn
- Mano Nicola
- Marchal Alexis
- Martineau Charles
- Mazzola Francesco
- Meloso Debrah
- Menkfeld Albert J.
- Mi Michael G.
- Mihet Roxana
- Mohan Vijay
- Moinas Sophie
- Moore David
- Mu Liangyi
- Muravyev Dmitriy
- Murphy Dermot
- Neszveda Gabor
- Neumeier Christian
- Neusüss Sebastian
- Nielsson Ulf
- Nimalendran Mahendrarajah
- Nolte Sven
- Norden Lars L.
- O'Neill Peter W.
- Obaid Khaled
- Pagnotta Emiliano
- Painter Marcus
- Palan Stefan
- Palit Imon J.
- Park Andreas
- Pascual Roberto
- Pasquariello Paolo
- Pastor Lubos
- Patel Vinay
- Patton Andrew J.
- Pearson Neil D.
- Pelizzon Loriana
- Pelli Michele
- Pelster Matthias
- Pfiffer Cameron
- Philip Richard
- Plíhal Tomáš
- Prakash Puneet
- Press Oliver-Alexander
- Prodromou Tina
- Prokopczuk Marcel
- Putnins Talis
- Pérignon Christophe
- Qian Ya
- Raizada Gaurav
- Rakowski David
- Ranaldo Angelo
- Razen Michael
- Regis Luca
- Reitz Stefan
- Renault Thomas
- Renjie Rex W.
- Reno Roberto
- Riddiough Steven J.
- Rinne Kalle
- Rintamäki Paul J.
- Riordan Ryan
- Rittmannsberger Thomas
- Rodríguez Longarela Iñaki
- Roesch Dominik
- Rognone Lavinia
- Roseman Brian
- Rosu Ioanid
- Roy Saurabh
- Rudolf Nicolas
- Rush Stephen R.
- Rzayev Khaladdin
- Rzeznik Aleksandra A.
- Sanford Anthony
- Sankaran Harikumar
- Sarkar Asani
- Sarno Lucio
- Scaillet Olivier
- Scharnowski Stefan
- Schenk-Hoppé Klaus R.
- Schertler Andrea
- Schneider Michael
- Schroeder Florian
- Schuster Philipp
- Schwarz Marco A.
- Schürhoff Norman
- Seasholes Mark S.
- Seeger Norman J.
- Shachar Or
- Shkilko Andriy
- Shui Jessica
- Sikic Mario
- Simion Giorgia
- Smales Lee A.
- Sojli Elvira
- Sokolov Konstantin
- Spokeviciute Laima
- Stefanova Denitsa
- Subrahmanyam Marti G.
- Szaszi Barnabas
- Söderlind Paul
- Sönksen Jantje
- Talavera Oleksandr
- Tang Yuehua
- Taylor Nick
- ter Ellen Saskia
- Tham Wing Wah
- Theissen Erik
- Thimme Julian
- Tonks Ian
- Tran Hai
- Trapin Luca
- Trolle Anders B.
- Vaduva M. Andreea
- Valente Giorgio
- van der Wel Michel
- van Dijk Mathijs A.
- Van Ness Robert A.
- Vasquez Aurelio
- Verousis Thanos
- Verwijmeren Patrick
- Vilhelmsson Anders
- Vilkov Grigory
- Vladimirov Vladimir
- Vogel Sebastian
- Voigt Stefan
- Wagner Wolf
- Walther Thomas
- Weiss Patrick
- Weitzel Utz
- Werner Ingrid M.
- Westerholm Joakim
- Westheide Christian
- Wika Hans C.
- Wipplinger Evert
- Wolf Michael
- Wolff Christian C. P.
- Wolk Leonard
- Wong Wink-Keung
- Wrampelmeyer Jan
- Wu Zhen-Xing
- Xia Shuo
- Xiu Dacheng
- Xu Caihong
- Xu Ke
- Yadav Pradeep K.
- Yagüe José
- Yan Cheng
- Yang Antti
- Yoo Woongsun
- Yu Shihao
- Yu Wenjia
- Yu Yihe
- Yueshen Bart Z.
- Yuferova Darya
- Zamojski Marcin
- Zareei Abalfazl
- Zeisberger Stefan M.
- Zhang Lu
- Zhang S. Sarah
- Zhang Xiaoyu
- Zhao Lu
- Zhong Zhuo
- Zhou Chen
- Zhou Zeyang (Ivy)
- Zhu Xingyu S.
- Zoican Marius
- Zwinkels Remco
- Östberg Per
- Ødegaard Bernt A.
- Publication venue
- Publication date
- 11/02/2023
- Field of study
In statistics, samples are drawn from a population in a data-generating process (DGP). Standard errors measure the uncertainty in estimates of population parameters. In science, evidence is generated to test hypotheses in an evidence-generating process (EGP). We claim that EGP variation across researchers adds uncertainty: Non-standard errors (NSEs). We study NSEs by letting 164 teams test the same hypotheses on the same data. NSEs turn out to be sizable, but smaller for better reproducible or higher rated research. Adding peer-review stages reduces NSEs. We further find that this type of uncertainty is underestimated by participants
Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries
- Author
- Aamir Faiqa Binte
- Aamir Syed Roohan
- Ababneh Adnan
- Abbal Muhammad Anas
- Abbas Ahmed M
- Abbas Mostafa
- Abd-Albain Montasir A
- Abdalei Abdulwahab
- Abdalla Ahmed
- Abdalla Ahmed Yassir
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- Wright Deborah
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- Wright Deborah
- Wu Alan
- Wu Alan
- Wu Chun-Yen
- Wu Hongyi
- Wycliff Daniel
- Xiao Haotian
- Xie Maria
- Xu Cindy
- Xu William
- Xu William
- Xu William
- Yaghmour Jamal
- Yahya Bilal Shuaibu
- Yahya Rawad
- Yang Wah
- Yao Michelle
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- Yasar Meltem
- Yasir Ruaa
- Yassin Mohamed
- Yavuz Dogancan
- Yavuz Ikranur
- Yaytokgil Muhammet
- Yazgan Yasemin
- Yee Su-Ann
- Yeung Justin
- Yeung Kar Long
- Yew Anthea
- Yi Kevin
- Yiannakopoulos Christos
- Yildirim Yasemin
- Yildiz Ahmet
- Yıldız Berkay
- Yilmaz Hakan
- Yin Chonghao
- Ying Geoffrey
- Yodkitydomying Chatnapa
- Yong Nathania
- Yoon Si Woo
- You Ha Seong
- Young Simon
- Young Stevie
- Younus Khadijah
- Yousif Fatima Imad
- Yousri Mohamed
- Youssef Ahmed A
- Youssef Arkan
- Yu Hoi Hang
- Yu Victor
- Yuan Grace
- Yue Bosco
- Yue Tiffany
- Yunos Mohamed Haseef Mohamed
- Yusuf Stephen
- Yuzgec Ayse Nilufer
- Zachomitros Fotios
- Zaed Moahmed
- Zafar Fatima
- Zaghloul Ahmed
- Zaheer Adil
- Zaidi Faseeh
- Zain Ahmad
- Zakaria Ahmed
- Zakaria Andee Dzulkarnaen
- Zakaria Mohd Zaim
- Zalloum Jihad
- Zamanbandhon Bushra
- Zambon Mattia
- Zapsalis Konstantinos
- Zargar Kamran
- Zarzour Mohamed A
- Zeng Raymond
- Zeng Raymond
- Zengin Kagan
- Zertuche Juventino Tadeo Guerrero
- Zhang Caitlin
- Zhang Edward
- Zhang Shane
- Zhang Yichi
- Zhao Hailin
- Zheng Helen
- Zhou Karen
- Zhou Yida
- Ziergiebel Felix
- Zou Peishan
- Zuylekom Kate Van
- Zuylekom Katie Van
- Zwain Yasir Adnan
- Zyul'korneeva Sof'ya
- Aladna Abdallah
- Aljoumaa Abdullah
- Nawfal Hamdi
- Niazi Ammar
- Publication venue
- Publication date
- 01/01/2024
- Field of study
Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely
p53 inhibition of AP1-dependent TFF2 expression induces apoptosis and inhibits cell migration in gastric cancer cells
- Author
- Publication venue
- American Physiological Society
- Publication date
- Field of study
Overexpression of trefoil factor 2 (TFF2) is associated with increased cell migration, resistance to apoptosis, and possibly increased gastric cancer invasion. Dysregulation of p53 is frequently observed in preneoplastic conditions of the stomach. Here, we investigated the effect of p53 on the expression and function of TFF2 in gastric cancer cell lines. Gene expression was determined by reverse transcription-polymerase chain reaction, and promoter activity was assessed by dual luciferase reporter assays. Apoptosis was detected by flow cytometry, and cell migration was evaluated by the Boyden chamber assay. Exogenous expression of p53 dose dependently inhibited endogenous TFF2 mRNA, protein, and promoter activity and resulted in induction of cell apoptosis and inhibition of cell migration. Downregulation of TFF2 by small interfering RNA sensitized gastric cancer cells to drug-induced p53-dependent apoptosis. Addition of human TFF2 peptide reversed p53-dependent apoptosis and inhibition of cell migration. The p53-responsive element was mapped to an AP-1-like cis-element at −182 bp upstream of the TFF2 transcription start site. Mutation of this AP-1-like element abrogated p53-mediated inhibition of TFF2 promoter activity. Gel shift and chromatin immunoprecipitation assays demonstrated that c-Jun and c-Fos bind to this AP-1-like element. Ectopic expression of c-Jun/c-Fos or p300 or treatment of cells with phorbol 12-myristate 13-acetate (PMA) stimulated endogenous TFF2 mRNA expression and promoter activity, and p53 inhibited the effects of AP-1 and PMA on TFF2. p53 induces cell apoptosis and inhibits cell migration in part by downregulating TFF2 expression through an AP-1-like site, suggesting that TFF2 may be an important downstream target of p53
The Nexus between Labor Wages and Property Rents in the Greater China Area
- Author
- Publication venue
- 'Elsevier BV'
- Publication date
- 01/01/2013
- Field of study
Crystal Structure of (.MU.-Thiocyanato-N, S) [bis-(3, 7, 11, 17-tetraazabicyclo[11, 3, 1]heptadec-1(17), 13, 15-triene-N, N, N", N)copper(II)] Trithiocyanate.
- Author
- Publication venue
- 'Japan Society for Analytical Chemistry'
- Publication date
- 01/01/2000
- Field of study
Time-variant 1D photonic crystals using flowing microdroplets
- Author
- Anna
- Brouzes
- Castro-Hernández
- Chi Wah Leung
- Chin
- Chin
- Chiou
- Christopher
- Cuennet
- Dittrich
- Dong
- Forsi
- Groisman
- Guo
- Hashimoto
- Helen L. W. Chan
- Homola
- Jakiela
- Kogelnik
- Lee
- Li
- Lien
- Lim
- Mancuso
- Mao
- Monat
- Neale
- Oskooi
- Prakash
- Psaltis
- Schmidt
- Schmidt
- Seemann
- Shopova
- Shui
- Shui
- Song
- Tan
- Tang
- Teh
- Theberge
- Thorsen
- Um
- Vahala
- Vlasov
- Wolfe
- Xiong
- Xu
- Xuming Zhang
- Yang
- Yang
- Yihang Chen
- Yu Wang
- Zefeng Chen
- Zehui Yong
- Zhang
- Zhu
- Publication venue
- 'The Optical Society'
- Publication date
- Field of study