Quantification of gliadin levels to the picogram level by flow cytometry

Celiac disease is a widely prevalent enteropathy caused by intolerance to gliadin, one of the gluten proteins. We developed two methods for the analysis of gliadin levels. Both methods use flow cytometry and rat antibodies against a 16-residue peptide of gliadin. The peptide is common to the alpha-, beta-, gamma-, and omega-gliadins

A note on polarized light from magnetars

In a recent paper it is claimed that vacuum birefringence has been experimentally observed for the first time by measuring the degree of polarization of visible light from a magnetar candidate, a neutron star with a magnetic field presumably as large as 10^13 G. The role of such a strong magnetic field is twofold. First, the surface of the star emits, at each point, polarized light with linear polarization correlated with the orientation of the magnetic field. Depending on the relative orientation of the magnetic axis of the star with the direction to the distant observer, a certain degree of polarization should be visible. Second, the strong magnetic field in the vacuum surrounding the star could enhance the effective degree of polarization observed: vacuum birefringence. We compare experimental data and theoretical expectations concluding that the conditions to support a claim of strong evidence of vacuum birefringence effects are not met

An HMO »Extended« Free Valence Index and its Application

An extended free valence index defined in the HMO framework and its modification is presented . It has been applied to several molecules containing heteroatoms and non-benzenoid systems, where the classical free valence index fails in predicting the reactivity positions. This index gives, by direct inspection, the most probable positions for electrophilic (ES) and nucleophilic (NS) substitutions

Neurobiological underpinnings of reward anticipation and outcome evaluation in gambling disorder

Gambling disorder is characterized by persistent and recurrent maladaptive gambling behavior, which leads to clinically significant impairment or distress. The disorder is associated with dysfunctions in the dopamine system. The dopamine system codes reward anticipation and outcome evaluation. Reward anticipation refers to dopaminergic activation prior to reward, while outcome evaluation refers to dopaminergic activation after reward. This article reviews evidence of dopaminergic dysfunctions in reward anticipation and outcome evaluation in gambling disorder from two vantage points: a model of reward prediction and reward prediction error by Wolfram Schultz et al. and a model of “wanting” and “liking” by Terry E. Robinson and Kent C. Berridge. Both models offer important insights on the study of dopaminergic dysfunctions in addiction, and implications for the study of dopaminergic dysfunctions in gambling disorder are suggested

An HMO »Extended« Free Valence Index and its Application

An extended free valence index defined in the HMO framework and its modification is presented . It has been applied to several molecules containing heteroatoms and non-benzenoid systems, where the classical free valence index fails in predicting the reactivity positions. This index gives, by direct inspection, the most probable positions for electrophilic (ES) and nucleophilic (NS) substitutions

Continuum enhancements, line profiles and magnetic field evolution during consecutive flares

During solar flares, magnetic energy can be converted into electromagnetic radiation from radio waves to $\gamma$ rays. Enhancements in the continuum at visible wavelengths give rise to white-light flares, as well as continuum enhancements in the FUV and NUV passbands. In addition, the strong energy release in these events can lead to the rearrangement of the magnetic field at the photospheric level, causing morphological changes in large and stable magnetic structures like sunspots. In this context, we describe observations acquired by satellite instruments (IRIS, SDO/HMI, Hinode/SOT) and ground-based telescopes (ROSA/DST) during two consecutive C7.0 and X1.6 flares occurred in active region NOAA 12205 on 2014 November 7. The flare was accompanied by an eruption. The results of the analysis show the presence of continuum enhancements during the evolution of the events, observed both in ROSA images and in \textit{IRIS} spectra. In the latter, a prominent blue-shifted component is observed at the onset of the eruption. We investigate the role played by the evolution of the $\delta$ sunspots of the active region in the flare triggering, and finally we discuss the changes in the penumbrae surrounding these sunspots as a further consequence of these flares.Comment: 19 pages, accepted for ApJ; some figures are in B/W to accomplish size limit

A novel synthetic peptide from a tomato defensin exhibits antibacterial activities against Helicobacter pylori

Defensins are a class of cysteine-rich proteins, which exert broad spectrum antimicrobial activity. In this work, we used a bioinformatic approach to identify putative defensins in the tomato genome. Fifteen proteins had a mature peptide that includes the well-conserved tetradisulfide array. We selected a representative member of the tomato defensin family; we chemically synthesized its gamma-motif and tested its antimicrobial activity. Here, we demonstrate that the synthetic peptide exhibits potent antibacterial activity against Gram-positive bacteria, such as Staphylococcus aureus A170, Staphylococcus epidermidis, and Listeria monocytogenes, and Gram-negative bacteria, including Salmonella enterica serovar Paratyphi, Escherichia coli, and Helicobacter pylori. In addition, the synthetic peptide shows minimal (<5%) hemolytic activity and absence of cytotoxic effects against THP-1 cells. Finally, SolyC exerts an anti-inflammatory activity in vitro, as it downregulates the level of the proinflammatory cytokines TNF-alpha and IFN-gamma