Inducción de la brotación en tubérculos de Ipomoea batatas mediante una mezcla de oligogalacturónidos bioactivos para su establecimiento in vitro

Tuberous  roots  of  sweet  potato,  belonging  to  clone  CEMSA  78-354,  coming  from  banks  of  seeds  and  collected, keeping  in  mind  their  sanity  and  uniformity  in  the  size,  were  used.  They  were  transferred  to  laboratory  under semicontrolled conditions and placed in glass flasks with 300 mg.l -1  of tap water (control treatment) and in flasks with the some volume of water and different pectimorf concentration (5, 10, 15 and 0 mg.l -1 ). At different time the following indicators: number of sprouts per tuber, length of sprouts and number of buds per sprouts. Evaluated indicators were achieved with the use of 15 mg.l -1  Pectimorf overcoming those reached by the control treatment.Key words: buds,  Pectimorf, sprouts, sweet potatoSe  emplearon  raíces  tuberosas  de  boniato  pertenecientes  al  clon  CEMSA  78-354,  procedentes  de  bancos  de semillas, las cuales fueron seleccionadas teniendo en cuenta su sanidad y uniformidad en el tamaño. Las mismas fueron trasladadas al laboratorio a condiciones semicontroladas y se colocaron en frascos de cristal con 300 ml de agua corriente (control) y en frascos con la misma cantidad de agua y diferentes concentraciones de Pectimorf (5, 10, 15 y 20 mg.l -1) . Se evaluaron en diferentes momentos los siguientes indicadores: número de brotes por tubérculo, longitud de los brotes y número de yemas por brote. De manera general, con el empleo de 15 mg.l -1  de Pectimorf se obtuvieron  los  mejores  resultados  en  los  indicadores  evaluados,  superando  los  alcanzados  por  el  control.Palabras clave: boniato, brotes, Pectimorf, yema

Evaluación del efecto del Pectimorf en el establecimiento in vitro de yemas axilares de boniato clon CEMSA 78-354

With the purpose of studying the effect of different Pectimorf dose in the establishment and budding of axillary buds of sweet potato starting from buds, tuberous roots belonging to the clone CEMSA 78-354 were collected and placed in flasks with water in the laboratory under semicontrolled conditions to induce the sprouting of the buds. Later on, axillary buds of the buds of the tubers (establishment) and axillary buds coming from in vitro plants (multiplication)were selected as sowing material (explants), which were sowed in the Murashige and Skoog culture medium containing the MS salts, thiamin (1 mg.l-1), myoinositol (100 mg.l-1), sucrose (3%), gelryte (2 g.l-1), giberelic acid (10.0 mg.l-1) and indol-3-acetic acid (0.05 mg.l-1) and the already mentioned culture medium without the previously refered regulators of the growth with different Pectimorf concentration: 5.0, 10.0 and 15.0 mg.l-1. The morphological behavior of the in vitro plantas was evaluated to the five, fifteen and thirty days after established the buds under in vitro conditions , and different variables related with the in vitro growth of the buds were analyzed, among which we have: number of sprouted buds, height of the in vitro plants (cm), number, longitude and width of the leaves, buds with roots, longitude of the roots (cm) and longitude of the petiole (cm). The best results were obtained when using the axillary buds coming from budding of tuberous roots in comparison with the axillary buds of the in vitro plants and with the use of the culture medium containing 10.0 mg.l-1 of Pectimorf.Key works: Ipomoea batatas, micropropagation, oligogalacturonidesCon la finalidad de estudiar el efecto de diferentes concentraciones de Pectimorf en el establecimiento in vitro de yemas axilares de boniato, fueron recolectadas raíces tuberosas pertenecientes al clon CEMSA 78-354 y se colocaron en frascos con agua en el laboratorio en condiciones semicontroladas para inducir la brotación de las yemas. Posteriormente, se seleccionaron como explantes para la siembra las yemas axilares de los brotes de los tubérculos (establecimiento) y yemas axilares procedentes de plantas in vitro (multiplicación), las cuales fueron sembradas en el medio de cultivo propuesto por Murashige y Skoog que contenía las sales MS, tiamina (1 mg.l-1), mioinositol (100 mg.l-1), sacarosa (3%), gelrite (2 g.l-1), ácido gibérelico (10.0 mg.l-1) y ácido indol-3-acético (0.05 mg.l-1) y este mismo medio de cultivo sin los reguladores del crecimiento antes mencionados, con diferentes concentraciones de Pectimorf: 5.0, 10.0 y 15.0 mg.l-1. Se evaluó el comportamiento morfológico de las plantas in vitro a los cinco, quince y treinta días después de establecidas las yemas en condiciones in vitro y se analizaron diferentes variables relacionadas con el crecimiento in vitro de las yemas: número de yemas brotadas, altura de las plantas in vitro (cm), número, longitud y ancho del limbo foliar (cm), número de yemas con raíces, longitud de las raíces (cm) y longitud de los peciolos (cm). Se obtuvieron los mejores resultados al emplear las yemas axilares procedentes de los brotes de raíces tuberosas en comparación con las yemas axilares de las plantas in vitro y con el empleo del medio de cultivo que contenía 10.0 mg.l-1 de Pectimorf.Palabras clave: Ipomoea batatas, micropropagación, oligogalacturónido

Iniciation in vitro of buds of Ipomoea batatas

Different buds of sweet potatowere studied. Roots of the clones Cemsa 78-354, Inivit 90-1, Inivit 93-1, Yabú-8 and Jewel were selected and put in glass with water in the laboratories for inducing the shoots. Afterwards the top shoot and the axiles shoots one to five (from top shoot ) were selected and sown in the Murashige and Skoog medium with the sales MS, Thiamine (1 mg.l-1), myoinositol (100 mg.l-1), sucrose (3%), gelrite (2 g.l-1) and different growth regulator combinations of giberelic acid (AG3) and 3-indol acetic acid (AIA), for example: AG3 (5 mg.l-1) + AIA (0.05 mg.l-1), AG3 (10 mg.l-1) + AIA (0.05 mg.l-1), AG3 (5 mg.l-1) + AIA (0.1 mg.l-1) and AG3 (10 mg.l-1) + AIA (0.1 mg.l-1). The pH was adjusted to 5.8. After five weeks, the morphologic behaviuor of the shoots was evaluated. The better results were obtained in the micropropagation with the use of the axiles shoots two, three and four in the culture medium whit growth regulator AG3 (10 mg.l-1) + AIA (0.05 mg.l-1). Key words: clones, axiles buds, top shoot, brotatio

Induction of sprouting in tubers of Ipomoea batatas by a mixture of bioactive oligogalacturonides for their In vitro establishment

Tuberous  roots  of  sweet  potato,  belonging  to  clone  CEMSA  78-354,  coming  from  banks  of  seeds  and  collected, keeping  in  mind  their  sanity  and  uniformity  in  the  size,  were  used.  They  were  transferred  to  laboratory  under semicontrolled conditions and placed in glass flasks with 300 mg.l -1  of tap water (control treatment) and in flasks with the some volume of water and different pectimorf concentration (5, 10, 15 and 0 mg.l -1 ). At different time the following indicators: number of sprouts per tuber, length of sprouts and number of buds per sprouts. Evaluated indicators were achieved with the use of 15 mg.l -1  Pectimorf overcoming those reached by the control treatment. Key words: buds,  Pectimorf, sprouts, sweet potat

Evaluation of the Pectiform effect in the in vitro establishment of axilary buds of sweet potato clone CENSA 78-354

With the purpose of studying the effect of different Pectimorf dose in the establishment and budding of axillary buds of sweet potato starting from buds, tuberous roots belonging to the clone CEMSA 78-354 were collected and placed in flasks with water in the laboratory under semicontrolled conditions to induce the sprouting of the buds. Later on, axillary buds of the buds of the tubers (establishment) and axillary buds coming from in vitro plants (multiplication)were selected as sowing material (explants), which were sowed in the Murashige and Skoog culture medium containing the MS salts, thiamin (1 mg.l-1), myoinositol (100 mg.l-1), sucrose (3%), gelryte (2 g.l-1), giberelic acid (10.0 mg.l-1) and indol-3-acetic acid (0.05 mg.l-1) and the already mentioned culture medium without the previously refered regulators of the growth with different Pectimorf concentration: 5.0, 10.0 and 15.0 mg.l-1. The morphological behavior of the in vitro plantas was evaluated to the five, fifteen and thirty days after established the buds under in vitro conditions , and different variables related with the in vitro growth of the buds were analyzed, among which we have: number of sprouted buds, height of the in vitro plants (cm), number, longitude and width of the leaves, buds with roots, longitude of the roots (cm) and longitude of the petiole (cm). The best results were obtained when using the axillary buds coming from budding of tuberous roots in comparison with the axillary buds of the in vitro plants and with the use of the culture medium containing 10.0 mg.l-1 of Pectimorf. Key works: Ipomoea batatas, micropropagation, oligogalacturonide