2 research outputs found
Beneficial Effects of Limosilactobacillus fermentum in the DCA Experimental Model of Irritable Bowel Syndrome in Rats
Limosilactobacillus fermentum CECT5716, a probiotic strain isolated from human milk, has
reported beneficial effects on different gastrointestinal disorders. Moreover, it has shown its ability to
restore altered immune responses, in association with microbiome modulation in different pathological
conditions. Therefore, our aim was to assess the effects of a Limosilacbacillus fermentum CECT5716
in a rat experimental model of irritable bowel syndrome (IBS) that resembles human IBS. The experimental
IBS was induced by deoxycholic acid (DCA) in rats and then, Limosilactobacillus fermentum
CECT5716 (109 CFU/day/rat) was administered. Behavioral studies, hyperalgesia and intestinal
hypersensitivity determinations were performed and the impact of the probiotic on the inflammatory
and intestinal barrier integrity was evaluated. Additionally, the gut microbiota composition was
analyzed. Limosilactobacillus fermentum CECT5716 attenuated the anxiety-like behavior as well as the
visceral hypersensitivity and referred pain. Moreover, this probiotic ameliorated the gut inflammatory
status, re-establishing the altered intestinal permeability, reducing the mast cell degranulation and
re-establishing the gut dysbiosis in experimental IBS. Therefore, our results suggest a potential use of
Limosilactobacillus fermentum CECT5716 in clinical practice for the management of IBS patients.Junta de Andalucia A-CTS-447-UGR18
CTS 164Instituto de Salud Carlos IIIEuropean Commission PI19/01058
PI20/0144
The Prebiotic Effects of an Extract with Antioxidant Properties from Morus alba L. Contribute to Ameliorate High-Fat Diet-Induced Obesity in Mice
Obesity is a global health issue, in which modifications in gut microbiota composition
have a key role. Different therapeutic strategies are being developed in combination with diet and
exercise, including the use of plant extracts, such as those obtained from Morus alba L. leaves. Recent
studies have revealed their anti-inflammatory and antioxidant properties. The aim of the present
work was to evaluate whether the beneficial effects of M. alba L. leaf extract in high-fat diet-induced
obesity in mice is correlated with its impact on gut microbiota. The extract reduced body weight
gain and attenuated lipid accumulation, as well as increased glucose sensitivity. These effects were
associated with an amelioration of the obesity-associated inflammatory status, most probably due
to the described antioxidant properties of the extract. Moreover, M. alba L. leaf extract mitigated
gut dysbiosis, which was evidenced by the restoration of the Firmicutes/Bacteroidota ratio and the
decrease in plasma lipopolysaccharide (LPS) levels. Specifically, the extract administration reduced
Alistipes and increased Faecalibaculum abundance, these effects being correlated with the beneficial
effects exerted by the extract on the obesity-associated inflammation. In conclusion, anti-obesogenic
effects of M. alba L. leaf extract may be mediated through the amelioration of gut dysbiosis.Junta de Andalucia CTS 164Instituto de Salud Carlos III PI19.01058Spanish Government AGL2015-67995-C3-3-Rperational Programme of the Region of Murcia (CCI) 2007ES161PO001
14-20/20European CommissionInstituto de Salud Carlos IIIi-pFIS, Instituto de Salud Carlos III; Programa de Doctorado BiomedicinapFIS, Instituto de Salud Carlos III; Programa de Doctorado Nutricio