Alterações físicas, fisiológicas e químicas durante o armazenamento de duas cultivares de sapoti

The objective of this work was to evaluate the physical, physiological and chemical changes during storage of two sapodilla cultivars (Manilkara sapota L.). Fruits were picked and evaluated at the physiological maturation point, and stored at temperature of 26±2ºC and 55±5% relative humidity. Respiration activities and ethylene production were daily evaluated, while the loss of mass, firmness, pulp color, total titratable acidity, pH, soluble solids content, total sugar and senescence were determined in the 0, 2, 4, 6, 8, 10 and 12 days of storage. Fruits of BRS-228 cultivar had respiration activities, ethylene production picks and senescence retarded when compared to BRS-227 cultivar. Fruits of BRS-228 maintained larger firmness, soluble solids and total sugar contents and smaller loss of mass than BRS-227. The cultivar BRS-228 presents higher postharvest storage potential than BRS-227.O objetivo deste trabalho foi avaliar alterações físicas, fisiológicas e químicas, durante a maturação de duas cultivares de sapoti (Manilkara sapota L.), relacionando-as às taxas respiratórias e de liberação de etileno. Os frutos foram colhidos no estádio de maturidade fisiológica, avaliados no dia da colheita e armazenados em temperatura de 26±2ºC e umidade relativa de 55±5%. A atividade respiratória e a produção de etileno foram determinadas diariamente; perda de massa, firmeza, acidez total titulável, pH, sólidos solúveis, açúcares solúveis totais e senescência foram avaliados nos tempos de 0, 2, 4, 6, 8, 10 e 12 dias de armazenamento. A cultivar BRS-228 teve pico respiratório, produção de etileno e senescência retardada em relação à cultivar BRS-227. A cultivar BRS-228 manteve maior firmeza, maiores teores de sólidos solúveis e açúcares solúveis totais, e menor perda de massa do que a cultivar BRS-227. A cultivar BRS-228 apresenta maior potencial de conservação pós-colheita do que a cultivar BRS-227

Enzyme activities and pectin breakdown of sapodilla submitted to 1-methylcyclopropene

O objetivo deste trabalho foi investigar a influência do 1-metilciclopropeno (1-MCP) nas atividades das enzimas hidrolíticas da parede celular e nas mudanças na degradação da pectina durante o amadurecimento de sapoti (Manilkara zapota cv. Itapirema 31). Frutos de sapotizeiro foram tratados com o inibidor da ação do etileno, 1-MCP, na concentração de 300 nL L-1, por 12 horas e armazenados sob atmosfera modificada, à temperatura de 25±2ºC, por 23 dias. A firmeza, conteúdo de pectina total e solúvel e enzimas da parede celular foram avaliados durante todo o período de armazenamento. O 1-MCP a 300 nL L-1 por 12 horas retardou significativamente o amolecimento de sapoti por 11 dias a 25ºC. O tratamento com 1-MCP afetou a atividade das enzimas pectinametilesterase e poligalacturonase e inibiu o aumento da atividade beta-galactosidase por 8 dias, e, conseqüentemente, resultou em menor solubilização das substâncias pécticas. A beta-galactosidase parece ser relevante no amolecimento de sapoti e responsável pela modificação das pectinas e das xiloglucanas ligadas as microfibrilas de celulose.The objective of this work was to investigate the influence of 1-methylcyclopropene (1-MCP) at 300 nL L-1 on activities of cell wall hidrolytic enzymes and pectin breakdown changes which Sapodilla (Manilkara zapota cv. Itapirema 31) cell wall undergoes during ripening. Sapodilla were treated with ethylene antagonist 1-MCP at 300 nL L-1 for 12 hours and then, stored under a modified atmosphere at 25ºC for 23 days. Firmness, total and soluble pectin and cell wall enzymes were monitored during storage. 1-MCP at 300 nL L-1 for 12 hours delayed significantly softening of sapodilla for 11 days at 25ºC. 1-MCP postharvest treatment affected the activities of cell wall degrading enzymes pectinmethylesterase and polygalacturonase and completely suppressed increases in beta-galactosidase for 8 days, resulting in less pectin solubilization. Beta-galactosidase seems relevant to softening of sapodilla and is probably responsible for modification of both pectin and xyloglucan-cellulose microfibril network

Effect of salt stress on muskmelon (Cucumis melo L.) seeds

Cucumis melo is one of the most appreciated and consumed vegetables in the world, however, producing areas have been suffering from increased salinity in irrigation water. Therefore, accesses of melon, from the germplasm bank of the Federal Rural Semi-Arid University (UFERSA), were tested for salinity and the physiological responses of the seedlings were evaluated. For the 24 accesses of C. melo, salinity of 0.06 and 3.45 dS m-1 was applied. For this, four subsamples of 25 seeds per access were used, which were seeded in polystyrene trays having as a substrate washed and sterilized sand, initially moistened at 50% field capacity. The design was completely randomized in a factorial scheme (two salinity levels and 24 accesses). Salinity affected the physiological quality (germination and mean germination time) of melon seeds, germinating on average 62%, with an average germination time of 4 days, mean height of 10.5 cm and reduction of dry matter accumulation. The saline treatment increased the EC of the soil saturation extract, indicating the presence of stress. The accesses that presented intolerance to salinity were: A35, A24, A41, A31, A09, A28 and A43. The moderately tolerant accesses were: A16, A19, A15, A17, A34, A25, A27, A18, A42. The salinity tolerant accesses were: A45, A08, A37, A50, A14, A36, A07, A39 which may serve as a basis for genetic improvement