The impact of Polybrene on GFP transfection into mouse adipose derived stem cells

Adipose tissue-derived stem cells (ADSCs) have been a promising candidate in regenerative therapies because of their self-renewal and differentiation capacity. They are not only easily harvested by minimally invasive techniques but can also be used as autologous transplantation. However, it is crucial to have more in-depth studies about these techniques before applying them to humans. Therefore, to have a better understanding and tracking of their behaviors inside the body, transfection of green fluorescentprotein (GFP) has been developed to provide a helpful tool for performing in vivo research. In this study, mouse ADSCs (mADSCs) were transfected by GFP lentivirus vector with polybrene (polybrene group) or without polybrene (non-polybrene group).The effect of polybrene on the transfection efficiency was evaluated by the expression of GFP through the glowing ability of transgenic cells, the percentage of glowing cells, and the doubling time of the cells. The results indicated that the gfp gene was successfully transferred into mADSCs of both polybrene and non-polybrene groups. However, the transfection efficiency of the non-polybrene group was higher than that of the polybrene group (86.2% > 71.13%). The doubling time of GFP-mADSCs in the non-polybrene group was equivalent to that of the normal mADSC group (32.5 hours ~ 32.64 hours); while the doubling time of GFP-mADSCs in the polybrene group was longer than the non-polybrene group’s and the control group’s (40.98 hours > 32.5 hours ~ 32.64 hours)

Văn hóa người Việt vùng Tây Nam Bộ

892 tr. : minh họa; 24 cm

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900 tr. : minh hoạ ; 24 cm