Different source of commercial vegetable oils may regulate metabolic, inflammatory and redox status in healthy rats.

Our goal was to carry out a comparative study to evaluate the metabolic and inflammatory effects and the redox status of commercial vegetable oils supplementation [linseed (LO), coconut (VCO), and sunflower (SO)] in metabolically healthy rats. The results found in this study showed that the LO group decreased the HOMA-IR and hepatic cholesterol, and increased the serum levels of IL-6. Supplementation with VCO increased glucose and HOMA-IR, cholesterol concentration and serum triacylglycerol (TAG). In this group, there was also an increase in TBARS. In the SO group there was a decrease in serum concentrations of cholesterol and TAG and an increase in hepatic concentration of these lipids. In addition, in the SO group there was a decrease in hepatic and s?rum concentrations of IL-6 and hepatic levels of TNF, as well as a decrease in the GSH/GSSG ratio, suggesting changes in glutathione metabolism and inflammatory mediators

Avalia??o das propriedades anti-inflamat?rias e antioxidantes de Morus nigra L. (blackberry) em um modelo murino de sepse induzido por LPS.

Programa de P?s-Gradua??o em Ci?ncias Biol?gicas. N?cleo de Pesquisas em Ci?ncias Biol?gicas, Pr?-Reitoria de Pesquisa de P?s Gradua??o, Universidade Federal de Ouro Preto.A sepse ? uma doen?a complexa, de alta incid?ncia nas unidades de terapia intensiva (UTIs) sendo a causa de muitos ?bitos no mundo inteiro. A patog?nese da sepse envolve uma resposta inflamat?ria desregulada com consequente produ??o de mediadores inflamat?rios e esp?cies reativas. A produ??o e libera??o excessiva dessas subst?ncias na circula??o sist?mica desencadeiam as diversas altera??es celulares e metab?licas que s?o observadas durante a evolu??o da doen?a. Alguns trabalhos cient?ficos tem investigado plantas como Morus nigra L., conhecida popularmente como amoreira-preta, pois sabe-se que as plantas desse g?nero apresentam um grande potencial terap?utico. Estudos j? mostraram que as plantas pertencentes a este g?nero s?o ricas em metab?litos secund?rios como os flavonoides que apresentam atividades biol?gicas importantes. Com base nesse contexto, o objetivo do nosso estudo foi avaliar as propriedades anti-inflamat?rias e antioxidantes de Morus nigra L. em modelo murino de sepse induzido por LPS. Para isso, camundongos machos da linhagem C57Bl/6, com oito semanas de idade e peso m?dio entre 20-25g foram distribu?dos em quatro grupos experimentais, a saber: controle (C), sepse (S), sepse tratado com extrato das folhas da amoreira (SF) e sepse tratado com a polpa de amora (SP). Os animais foram tratados com 100?l de seus respectivos tratamentos, por gavagem, durante vinte e um dias. A sepse foi induzida no 21? atrav?s de inje??o intraperitoneal com lipopolissacar?deo (LPS). Os animais foram eutanasiados 24 horas ap?s receberem a inje??o de LPS e amostras de plasma, f?gado e lavado bronco-alveolar foram coletados e armazenados para an?lises posteriores. Os dados obtidos a partir das dosagens laboratoriais foram analisados no software GraphPad Prism 5.0 para Windows (San Diego, California, USA). Nossos resultados mostraram que os tratamentos com a polpa e o extrato das folhas diminu?ram significativamente o infiltrado de leuc?citos nos pulm?es e os n?veis s?ricos de TNF nos animais s?pticos. Com rela??o aos marcadores de processos redox, ambos os tratamentos diminu?ram significativamente a atividade antioxidante da enzima glutationa peroxidase (GPx). No que diz respeito ? metaloproteinase do tipo 2 (MMP-2) observamos que o tratamento com a polpa da amora foi capaz de reduzir significativamente a atividade dessa enzima em rela??o ao grupo sepse. Por fim, esses resultados positivos em conjunto, promoveram o aumento na sobrevida dos animais s?pticos que receberam o extrato das folhas ou a polpa de amora.Sepsis is a complex disease of high incidence in intensive care units (ICUs) and is the cause of many deaths worldwide. The pathogenesis of sepsis involves a deregulated inflammatory response with consequent production of inflammatory mediators and reactive species. The production and excessive release of these substances into the systemic circulation trigger the various cellular and metabolic alterations that are observed during the evolution of the disease. Some scientific works have investigated plants like Morus nigra L., popularly known as blackberry, because it is known that plants of this genus have great therapeutic potential. Studies have shown that plants belonging to this genus are rich in secondary metabolites such as flavonoids that have important biological activities. Based on this context, the objective of our study was to evaluate the anti-inflammatory and antioxidant properties of Morus nigra L. in murine model of sepsis induced by LPS. For this, male mice of the lineage C57Bl / 6, with eight weeks of age and average weight between 20-25g were distributed in four experimental groups: control (C), sepsis (S), sepsis treated with leaf extract mulberry (SL) and sepsis treated with mulberry pulp (SP). The animals were treated with 100?l of their respective treatments, by gavage, for twenty one days. Sepsis was induced at the 21st through intraperitoneal injection with lipopolysaccharide (LPS). The animals were euthanized 24 hours after receiving the LPS injection and plasma, liver and bronchoalveolar lavage samples were collected and stored for further analysis. The data obtained from the laboratory dosages were analyzed in GraphPad Prism 5.0 software for Windows (San Diego, California, USA). Our results showed that treatments with pulp and leaf extract significantly decreased leukocyte infiltrate in the lungs and serum TNF levels in septic animals. Regarding the markers of redox processes, both treatments significantly decreased the antioxidant activity of the enzyme glutathione peroxidase (GPx). As regards metalloproteinase type 2 (MMP-2), we observed that the treatment with the blackberry pulp was able to significantly reduce the activity of this enzyme in relation to the sepsis group. Finally, these positive results together promoted an increase in the survival of the septic animals that received the leaf extract or the blackberry pulp

Baccharis trimera protects against ethanol induced hepatotoxicity in vitro and in vivo.

Ethnopharmacological relevance: Baccharis trimera has been traditionally used in Brazil to treat liver diseases. Aim of the study: To evaluate the protective effect of Baccharis trimera in an ethanol induced hepatotoxicity model. Materials and methods: The antioxidant capacity was evaluated in vitro by the ability to scavenged the DPPH radical, by the quantification of ROS, NO and the transcription factor Nrf2. Hepatotoxicity was induced in animals by administration of absolute ethanol for 2 days (acute) or with ethanol diluted for 28 days (chronic). The biochemical parameters of hepatic function (ALT and AST), renal function (urea and creatinine) and lipid profile (total cholesterol, triglycerides and HDL) were evaluated. In addition to antioxidant defense (SOD, catalase, glutathione), oxidative damage markers (TBARS and carbonylated protein), MMP-2 activity and liver histology. Results: Baccharis trimera promoted a decrease in ROS and NO, and at low concentrations promoted increased transcription of Nrf2. In the acute experiment it promoted increase of HDL, in the activity of SOD and GPx, besides diminishing TBARS and microesteatosis. Already in the chronic experiment B. trimera improved the hepatic and renal profile, decreased triglycerides and MMP-2 activity, in addition to diminishing microesteatosis. Conclusion: We believe that B. trimera action is possibly more associated with direct neutralizing effects or inhibition of reactive species production pathways rather than the modulation of the antioxidant enzymes activity. Thus it is possible to infer that the biological effects triggered by adaptive responses are complex and multifactorial depending on the dose, the time and the compounds used