Magnetic brain stimulation upregulates adhesion and prevents EAE: MMP-2, ICAM-1, and VCAM-1 in the choroid plexus as a target

We are most thankful to Prof. Yehuda Shoenfeld, head of Autoimmunity Center, Sheba Hospital at Tel Aviv, University in Israel, for critical and constructive comments on the manuscript. Thanks to Prof. B. Reljin and N. Stepanic, School of Informational Technology, Universirty of Belgrade for contribution in molecular images and computing analysis. Authors gratefully acknowledge the repeated gifts of the magnetic beads for magnetic brain stimulation of rats presented by Institute for Nuclear Physics, Vincha, Belgrade. Clinical signs appearance and significant increases of ICAM-1 and MMP-2 expressions with the clusters of VCAM-1(+) immunoreactivity in the choroids plexus epithelium to transferred anti-myelin oligodendroglial antibodies into the third brain ventricle, indicate important role of choroids plexus in the induction of acute experimental autoimmune encephalomyelitis (EAE). Magnetic brain stimulation with AKMA micro-magnet flux density of 60 miliTesla, 5 mm in diameter, implanted upon the pineal gland (PG), immediately after antibody injection, significantly decreases the expression of MMP-2 and ICAM-1 in the choroids plexus of the rat brain and abruptly suppresses the induction of acute EAE