High Temperature Creep Behaviour of Cast Nickel-Based Superalloys INC 713 LC, B1914 and MAR-M247

Cast nickel-based superalloys INC713 LC, B1914 and MAR-M247 are widely used for high temperature components in the aerospace, automotive and power industries due to their good castability, high level of strength properties at high temperature and hot corrosion resistance. The present study is focused on the mutual comparison of the creep properties of the above-mentioned superalloys, their creep and fracture behaviour and the identification of creep deformation mechanism(s). Standard constant load uniaxial creep tests were carried out up to the rupture at applied stress ranging from 150 to 700 MPa and temperatures of 800–1000 °C. The experimentally determined values of the stress exponent of the minimum creep rate, n, were rationalized by considering the existence of the threshold stress, σ0. The corrected values of the stress exponent correspond to the power-law creep regime and suggest dislocation climb and glide as dominating creep deformation mechanisms. Fractographic observations clearly indicate that the creep fracture is a brittle mostly mixed transgranular and intergranular mode, resulting in relatively low values of fracture strain. Determined main creep parameters show that the superalloy MAR-M247 exhibits the best creep properties, followed by B1914 and then the superalloy INC713 LC. However, that each of the investigated superalloys can be successfully used for high temperature components fulfils the required service loading conditions

Influence of High Pressure Sliding and Rotary Swaging on Creep Behavior of P92 Steel at 500 °C

High-pressure sliding (HPS) and rotary swaging (RS) at room temperature were used to form severely deformed microstructures in martensitic creep-resistant P92 steel. The deformed microstructures contained markedly different ratios of low- and high-angle grain boundaries (LAGBs/HAGBs). The application of the RS method, with an imposed equivalent strain of 1.4, led to the formation of a heterogeneous microstructure with a high number of LAGBs, while the HPS method, with an imposed equivalent strain of 7.8, led to the formation of a relatively homogeneous ultrafine-grained microstructure with a significant predominance of HAGBs. Microstructure analyses after creep testing showed that the microstructure of RS- and HPS-processed P92 steel is quite stable, but a slight coarsening of subgrains and grains during creep testing can be observed. Constant load tensile creep tests at 500 °C and initial stresses ranging from 300 to 900 MPa revealed that the specimens processed by HPS exhibited higher creep strength (slower minimum creep rate) and ductility compared to the coarse-grained and RS-processed P92 steel. However, the HPS-processed P92 steel also exhibited lower values of stress exponent n than the other investigated states of P92 steel. For this reason, the differences in minimum creep rates determined for different states decrease with decreasing values of applied stress, and at applied stresses lower than 500 MPa, the creep resistance of the RS-processed state is higher than the creep resistance of the HPS-processed state

Influence of High Pressure Sliding and Rotary Swaging on Creep Behavior of P92 Steel at 500 °C

High-pressure sliding (HPS) and rotary swaging (RS) at room temperature were used to form severely deformed microstructures in martensitic creep-resistant P92 steel. The deformed microstructures contained markedly different ratios of low- and high-angle grain boundaries (LAGBs/HAGBs). The application of the RS method, with an imposed equivalent strain of 1.4, led to the formation of a heterogeneous microstructure with a high number of LAGBs, while the HPS method, with an imposed equivalent strain of 7.8, led to the formation of a relatively homogeneous ultrafine-grained microstructure with a significant predominance of HAGBs. Microstructure analyses after creep testing showed that the microstructure of RS- and HPS-processed P92 steel is quite stable, but a slight coarsening of subgrains and grains during creep testing can be observed. Constant load tensile creep tests at 500 °C and initial stresses ranging from 300 to 900 MPa revealed that the specimens processed by HPS exhibited higher creep strength (slower minimum creep rate) and ductility compared to the coarse-grained and RS-processed P92 steel. However, the HPS-processed P92 steel also exhibited lower values of stress exponent n than the other investigated states of P92 steel. For this reason, the differences in minimum creep rates determined for different states decrease with decreasing values of applied stress, and at applied stresses lower than 500 MPa, the creep resistance of the RS-processed state is higher than the creep resistance of the HPS-processed state

Creep Resistance of S304H Austenitic Steel Processed by High-Pressure Sliding

Sheets of coarse-grained S304H austenitic steel were processed by high-pressure sliding (HPS) at room temperature and a ultrafine-grained microstructure with a mean grain size of about 0.14 µm was prepared. The microstructure changes and creep behavior of coarse-grained and HPS-processed steel were investigated at 500–700 °C under the application of different loads. It was found that the processing of S304H steel led to a significant improvement in creep strength at 500 °C. However, a further increase in creep temperature to 600 °C and 700 °C led to the deterioration of creep behavior of HPS-processed steel. The microstructure results suggest that the creep behavior of HPS-processed steel is associated with the thermal stability of the SPD-processed microstructure. The recrystallization, grain growth, the coarsening of precipitates led to a reduction in creep strength of the HPS-processed state. It was also observed that in the HPS-processed microstructure the fast formation of σ-phase occurs. The σ-phase was already formed during slight grain coarsening at 600 °C and its formation was enhanced after recrystallization at 700 °C

Validated WGS and WES protocols proved saliva-derived gDNA as an equivalent to blood-derived gDNA for clinical and population genomic analyses

Abstract Background Whole exome sequencing (WES) and whole genome sequencing (WGS) have become standard methods in human clinical diagnostics as well as in population genomics (POPGEN). Blood-derived genomic DNA (gDNA) is routinely used in the clinical environment. Conversely, many POPGEN studies and commercial tests benefit from easy saliva sampling. Here, we evaluated the quality of variant call sets and the level of genotype concordance of single nucleotide variants (SNVs) and small insertions and deletions (indels) for WES and WGS using paired blood- and saliva-derived gDNA isolates employing genomic reference-based validated protocols. Methods The genomic reference standard Coriell NA12878 was repeatedly analyzed using optimized WES and WGS protocols, and data calls were compared with the truth dataset published by the Genome in a Bottle Consortium. gDNA was extracted from the paired blood and saliva samples of 10 participants and processed using the same protocols. A comparison of paired blood–saliva call sets was performed in the context of WGS and WES genomic reference-based technical validation results. Results The quality pattern of called variants obtained from genomic-reference-based technical replicates correlates with data calls of paired blood–saliva-derived samples in all levels of tested examinations despite a higher rate of non-human contamination found in the saliva samples. The F1 score of 10 blood-to-saliva-derived comparisons ranged between 0.8030–0.9998 for SNVs and between 0.8883–0.9991 for small-indels in the case of the WGS protocol, and between 0.8643–0.999 for SNVs and between 0.7781–1.000 for small-indels in the case of the WES protocol. Conclusion Saliva may be considered an equivalent material to blood for genetic analysis for both WGS and WES under strict protocol conditions. The accuracy of sequencing metrics and variant-detection accuracy is not affected by choosing saliva as the gDNA source instead of blood but much more significantly by the genomic context, variant types, and the sequencing technology used