1 research outputs found
Nanodiamond quantum sensors reveal temperature variation associated to hippocampal neurons firing
Temperature is one of the most relevant parameters for the regulation of
intracellular processes. Measuring localized subcellular temperature gradients
is fundamental for a deeper understanding of cell function, such as the genesis
of action potentials, and cell metabolism. Here, we detect for the first time
temperature variations (1{\deg}C) associated with potentiation and depletion of
neuronal firing, exploiting a nanoscale thermometer based on optically detected
magnetic resonance in nanodiamonds. Our results provide a tool for assessing
neuronal spiking activity under physiological and pathological conditions and,
conjugated with the high sensitivity of this technique (in perspective
sensitive to < 0.1{\deg}C variations), pave the way to a systematic study of
the generation of localized temperature gradients. Furthermore, they prompt
further studies explaining in detail the physiological mechanism originating
this effect.Comment: 27 pages, 5 figures, 3 table