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HPLC determination of flavonoids in hairy-root cultures of Scutellaria baicalensis Georgi
A simple and sensitive liquid chromatographic method has been
established for determination of the main flavonoid components of
Scutellaria boicolensis Georgi (Lamiaceae) roots and hairy-root
cultures. Wogon, the dried root of the plant, is used in traditional
Chinese medicine for treatment of bronchitis, hepatitis, tumors, and
inflammatory diseases.
Lyophilized hairy roots were extracted with methanol. The crude
extracts were purified by SPE on Supelco LC-8 cartridges. HPLC
separations were performed on a Eurospher 100-C-8 reversed-phase
column. The mobile phase was a gradient prepared from mixtures of
acetonitrile and 0.1% trifluoroacetic acid. Peaks were identified by
addition of standards and/or by diode-array detection. Baicalein
7-O-glucuronide (baicalin), wogonin 7-O-glucuronide (wogonoside),
baicalein, wogonin, and acteoside were determined by the external
standard method at 280 nm. We found that the aglycon (baicalein and
wogonin) content of the transformed roots was consistently higher than
that of the intact root from Siberia