Resolving RAD51C function in late stages of homologous recombination

DNA double strand breaks are efficiently repaired by homologous recombination. One of the last steps of this process is resolution of Holliday junctions that are formed at the sites of genetic exchange between homologous DNA. Although various resolvases with Holliday junctions processing activity have been identified in bacteriophages, bacteria and archaebacteria, eukaryotic resolvases have been elusive. Recent biochemical evidence has revealed that RAD51C and XRCC3, members of the RAD51-like protein family, are involved in Holliday junction resolution in mammalian cells. However, purified recombinant RAD51C and XRCC3 proteins have not shown any Holliday junction resolution activity. In addition, these proteins did not reveal the presence of a nuclease domain, which raises doubts about their ability to function as a resolvase. Furthermore, oocytes from infertile Rad51C mutant mice exhibit precocious separation of sister chromatids at metaphase II, a phenotype that reflects a defect in sister chromatid cohesion, not a lack of Holliday junction resolution. Here we discuss a model to explain how a Holliday junction resolution defect can lead to sister chromatid separation in mouse oocytes. We also describe other recent in vitro and in vivo evidence supporting a late role for RAD51C in homologous recombination in mammalian cells, which is likely to be resolution of the Holliday junction

Correlations between X-ray Spectral and Timing Characteristics in Cyg X-2

Correlations between the quasi-periodic oscillations (QPOs) and the spectral power-law index have been reported for a number of black hole candidate sources and for four neutron star (NS) sources, 4U 0614+09, 4U 1608-52, 4U 1728-34 and Sco X-1. An examination of QPO frequencies and index relationship in Cyg X-2 is reported herein. The RXTE spectrum of Cyg X-2 can be adequately represented by a simple two-component model of Compton up-scattering with a soft photon electron temperature of about 0.7 keV and an iron K-line. Inferred spectral power-law index shows correlation with the low QPO frequencies. We find that the Thomson optical depth of the Compton cloud (CC) tau, in framework of spherical geometry, is in the range of ~4-6, which is consistent with the neutron star's surface being obscured. The NS high frequency pulsations are presumably suppressed as a result of photon scattering off CC electrons because of such high values of tau. We also point out a number of similarities in terms timing (presence of low and high frequency QPOs) and spectral (high CC optical depth and low CC plasma temperature) appearances between Cyg X-2 and Sco X-1.Comment: 7 pages, 4 figures, accepted for publication in ApJ (October 1, 2007, v667n2 issue

Hyperbolic chaos in self-oscillating systems based on mechanical triple linkage: Testing absence of tangencies of stable and unstable manifolds for phase trajectories

Dynamical equations are formulated and a numerical study is provided for self-oscillatory model systems based on the triple linkage hinge mechanism of Thurston -- Weeks -- Hunt -- MacKay. We consider systems with holonomic mechanical constraint of three rotators as well as systems, where three rotators interact by potential forces. We present and discuss some quantitative characteristics of the chaotic regimes (Lyapunov exponents, power spectrum). Chaotic dynamics of the models we consider are associated with hyperbolic attractors, at least, at relatively small supercriticality of the self-oscillating modes; that follows from numerical analysis of the distribution for angles of intersection of stable and unstable manifolds of phase trajectories on the attractors. In systems based on rotators with interacting potential the hyperbolicity is violated starting from a certain level of excitation.Comment: 30 pages, 18 figure

A handcuff model for the cohesin complex

The cohesin complex is responsible for the accurate separation of sister chromatids into two daughter cells. Several models for the cohesin complex have been proposed, but the one-ring embrace model currently predominates the field. However, the static configuration of the embrace model is not flexible enough for cohesins to perform their functions during DNA replication, transcription, and DNA repair. We used coimmunoprecipitation, a protein fragment complement assay, and a yeast two-hybrid assay to analyze the protein–protein interactions among cohesin subunits. The results show that three of the four human cohesin core subunits (Smc1, Smc3, and Rad21) interact with themselves in an Scc3 (SA1/SA2)-dependent manner. These data support a two-ring handcuff model for the cohesin complex, which is flexible enough to establish and maintain sister chromatid cohesion as well as ensure the fidelity of chromosome segregation in higher eukaryotes

Synchronisation schemes for two dimensional discrete systems

In this work we consider two models of two dimensional discrete systems subjected to three different types of coupling and analyse systematically the performance of each in realising synchronised states.We find that linear coupling effectively introduce control of chaos along with synchronisation,while synchronised chaotic states are possible with an additive parametric coupling scheme both being equally relevant for specific applications.The basin leading to synchronisationin the initial value plane and the choice of parameter values for synchronisation in the parameter plane are isolatedin each case.Comment: 17 pages 8 figures. submitted to physica script