Managing the motivation provision of universities in the context of the educational process digitalisation

The paper proposes a method for increasing the motivation of university teachers to work with digital technologies on the example of teaching foreign languages. The fear of complete displacement of the teacher by digital technologies leads to many ethical and psychological problems, resulting in a reluctance to use training programmes in the educational process. To increase the motivation of teachers to work in the digital environment, it is proposed to involve them in the use of digital educational technologies to adapt the work plans of disciplines to the preparation level of students entering universities. The specialists team composition required for the implementation of this method in practice has been formed. Additional factors for increasing the teachers motivation to work in a digital environment when working in a team have been shown. The proposed approach can be used in the implementation in digital educational systems of educational institutions

Yield formation of PVY-resistant and susceptible potato breeding lines

We studied the resistance to potato virus Y (PVY) in a potato hybrid population, and its effect on the yield formation under contrasting meteorological conditions. The virus resistance analysis was conducted after growing the plants under natural virus infection and after PVY inoculation. The dominant Ryadg gene allele in the potato breeding lines genotypes was revealed by detection of the PCR-based molecular marker RYSC3. Virus resistant lines were selected more often in the potato breeding program than susceptible forms due to higher productivity and larger number of tubers. PVY-resistant samples demonstrated a higher yield than susceptible forms. Significant differences (p < 0.05) were detected by univariate ANOVA in three of the four years studied. The virus resistance effect value ranged from 4 to 22%. Resistant form yield was more stable than that of susceptible one, which was evident from the corresponding coefficients of variation. Resistant samples also had a greater number of tubers per plant. At the same time, univariate ANOVA revealed significant differences (p < 0.05) only in one of the four studied years. The virus resistance effect ranged from 2 to 22%

Gradient soft magnetic materials produced by additive manufacturing from non-magnetic powders

The changes in the magnetic properties have been attributed to the formation of the BCC phase after mixing two FCC-dominated powders. Moreover, the phase composition of the obtained gradient materials can be predicted with reasonable accuracy by the CALPHAD approach, thus providing efficient optimization of the performance. The obtained results provide new prospects for printing gradient magnetic alloy

Effect of scanning strategy on mechanical properties of Ti-6Al-4V alloy manufactured by laser direct energy deposition

In this work, we investigated the effect of DED thermal history on the obtained structural and mechanical properties of Ti-6Al-4V using a thermocouple. As a result of the study, the specific DED process parameters allow the same mechanical characteristics for as-built titanium alloy and the alloy after heat treatmen

Phase and structural changes during heat treatment of additive manufactured CrFeCoNi high-entropy alloy

In the current study, the effect of a post-printing heat treatment at 400-1000 ℃ for 24 h and for 21 days on the changes in structures and phase compositions of an AM CrFeCoNi alloy prepared by the laser powder bed fusion AM technique is presented to better understand a heat treatment-microstructure-property relationship of the AM HE

Hormonal characteristics of androgen status and their relationship with the anthropometric and metabolic parameter in men depending on the severity of hypertension

Background. According to the literature data, long-term and poorly controlled hypertension (HTN) leads to target organs damage, and in some cases, to the development of associated diseases, the severity of which in men may be partly due to the characteristics of the androgenic status.The aim: to reveal the features of hormonal indicators of androgen status and their relationship with the anthropometric and metabolic characteristics of the therapeutic clinic male patients, depending on the severity of HTN.Materials and methods. 296 men aged 59,0 [54,0; 65,0] years with confirmed HTN were examined. All study participants in the morning on an empty stomach underwent anthropometry, determination of metabolic and androgenic status, and assessment of comorbid burden. Depending on the severity of hypertension, all patients were divided into two groups (G): G1 (n = 134) – patients with hypertension without a history of coronary artery disease or stroke; G2 (n = 162) – patients with hypertension with a history of ischemic heart disease (IHD) and/or stroke.Results. In male patients of G2 with severe HTN, a decrease in the levels of free testosterone (FT) and, to a greater extent, dehydroepiandrosterone sulfate (DHEA-S), as well as total cholesterol, high and low density lipoproteins, was revealed. The correlation analysis performed revealed statistically significant negative correlations between the levels of total testosterone (TT) and FT with body mass index, the value of the ratio of waist circumference to hips, only the level of FT – with the presence of IHD, chronic heart failure (CHF), disorders of carbohydrate metabolism and abdominal type of fat distribution, and the level of DHEA-S – with the presence of IHD and CHF.Conclusion. A decrease in FT and DHEA-S is associated with early progression of atherosclerosis and the risk of developing HTN-associated cardiovascular events, which can aggravate the course of HTN

BIOINFORMATIC SEARCH OF CRISPR/CAS SYSTEM STRUCTURES IN GENOME OF PCT281 PLASMID OF BACILLUS THURINGIENSIS SUBSP. CHINENSIS STRAIN CT-43

Background. CRISPR/Cas systems loci are one of the functionally important patterns in bacterial genome which perform the role of “adaptive immune defense” from foreign nucleic acids. The study of CRISPR/Cas systems structure in genomes of plasmids and phages provide new information about the evolution of this systems in bacterial hosts.Aims. A search of CRISPR/Cas systems structures in pCT281 plasmid from Bacillus thuringiensis subsp. chinensis strain CT-43 using bioinformatic methods.Materials and methods. Search studies using bioinformatics methods were performed with the genome of pCT281 plasmid of B. thuringiensis subsp. chinensis strain CT-43 from the RefSeq database. To search for the CRISPR/Cas system structure MacSyFinder (ver. 1.0.5) and three combined algorithms were used: CRISPRFinder; PILER-CR; CRISPR Recognition Tool (CRT). The consensus repeat sequence was generated in WebLogo 3.Results and discussion. In pCT281 plasmid we detected one locus of CRISPR/Cas system of the type I-C which contains 2 CRISPR-cassettes and 4 cas-genes located between them. The CRISPR-cassette 1 includes 10 spacers from 32 to 35 bp and 11 repeats 32bp in length. 5 spacers (33–35 bp) separated by 6 repeats 32 bp in length were detected in the CRISPR-cassette 2.Conclusions. The bioinformatic methods used in this study enable to conduct a search of CRISPR/Cas systems structures in plasmid genomes. The presence of the CRISPR-Cas locus in pCT281 plasmid confirms a possible transfer of this system from the nucleoid to this plasmid. The detected spacers provide information about phages this bacteria was encountered

BIOINFORMATION SEARCH AND ANALYSIS OF STRUCTURES OF CRISPR/CAS SYSTEMS IN PHAGE STAPHYLOCOCUS AUREUS GENOME AND ESTIMATION OF PROFILES OF PHAGE DETECTED THROUGH CRISPR-CASSETTE BACTERIA

The emergence of resistance among the most important bacterial pathogens is generally recognized as one of the major public health problems. The most important of these organisms are penicillin-resistant Streptococcus pneumoniae, vancomycin-resistant enterococci and methicillin- and vancomycin-resistant Staphylococcus aureus. These antibiotic resistance in common pathogens have made antimicrobial therapy of many infections. Scientists need to look for new ways of treating bacterial infections in the work, using the developed algorithm from the methods of search software in the genomic structure of Staphylococcus aureus subsp. aureus ST228, the CRISPR/Cas locus and the division structures of its CRISPR cassette. The results of the bacteriophage search through the decoded spacer sequences of CRISPR-cassettes of this strain were also obtained using the developed algorithm of the software methods of bioinformatics. It was determined that the CRISPR/Cas system of strain of ST228 of S. aureus was of type IIIA. It is shown that cas-genes are in the immediate vicinity of CRISPR cassettes. The spacer structures in the detected CRISPR cassette are the Staphylococcus, Mycobacterium, Streptococcus, Bacillus, Gordonia, Arthrobacter, Streptomyces. The implementation of the algorithm of program methods for locating CRISPR/Cas-loci can be applied to many other decoded bacterial genomes to return bacteriophage therapy

Financial Strategy of Enterprises: Formulation and Results over 2000—2009 (the Khabarovsk Territory’s example)

The article considers the concepts of capital and capital management strategies in the non-financial economic sector, as well as the need for analytical studies in this area in terms of strategic interests of the regional economy. Monitoring of enterprises as a methodological basis for analytical studies of the regional economy and financial strategies of enterprises is conducted. Factors that shape the differences in financial strategies at the level of enterprise groups of certain types and range of activity are identified. The efficiency of strategies and their impact on the regional economy as a whole is state