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ADVANCED CONTROL OF GLYCOSYLATION AND TITER IN FED-BATCH MONOCLONAL ANTIBODY PRODUCTION

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Studies on Composite Extrudable Propellant with varied Burning Rate Pressure Index 'n'

This paper discusses the development of composite propellantextrusion technique and the study of burning rate pressure indices nwith respect to compositional variations. The n is found to vary from0.35 to plateau and plateau to mesa by suitable compositionalmodifications. Compositional influence on burning rate with specificreference to plateau and mesaburning additives is described. Detailsof the process parameters like fluidity of the slurry, extrusion pressure,extrusion rate and die-swell are presented. This propellant is based onISRO-CTPB binder using ISRO-AP as oxidizer. Ammonium perchlorate (AP) particle size variation and inclusion of additives likePVC, lead stearate, ammonium sulphate, lithium fluoride etc. are foundto influence the burning rate pressure index n

Towards Real-Time Oxygen Sensing: From Nanomaterials to Plasma

A significantly large scope is available for the scientific and engineering developments of high-throughput ultra-high sensitive oxygen sensors. We give a perspective of oxygen sensing for two physical states of matters—solid-state nanomaterials and plasma. From single-molecule experiments to material selection, we reviewed various aspects of sensing, such as capacitance, photophysics, electron mobility, response time, and a yearly progress. Towards miniaturization, we have highlighted the benefit of lab-on-chip-based devices and showed exemplary measurements of fast real-time oxygen sensing. From the physical–chemistry perspective, plasma holds a strong potential in the application of oxygen sensing. We investigated the current state-of-the-art of electron density, temperature, and design issues of plasma systems. We also show numerical aspects of a low-cost approach towards developing plasma-based oxygen sensor from household candle flame. In this perspective, we give an opinion about a diverse range of scientific insight together, identify the short comings, and open the path for new physical–chemistry device developments of oxygen sensor along with providing a guideline for innovators in oxygen sensing

Towards Real-Time Oxygen Sensing: From Nanomaterials to Plasma

A significantly large scope is available for the scientific and engineering developments of high-throughput ultra-high sensitive oxygen sensors. We give a perspective of oxygen sensing for two physical states of matters—solid-state nanomaterials and plasma. From single-molecule experiments to material selection, we reviewed various aspects of sensing, such as capacitance, photophysics, electron mobility, response time, and a yearly progress. Towards miniaturization, we have highlighted the benefit of lab-on-chip-based devices and showed exemplary measurements of fast real-time oxygen sensing. From the physical–chemistry perspective, plasma holds a strong potential in the application of oxygen sensing. We investigated the current state-of-the-art of electron density, temperature, and design issues of plasma systems. We also show numerical aspects of a low-cost approach towards developing plasma-based oxygen sensor from household candle flame. In this perspective, we give an opinion about a diverse range of scientific insight together, identify the short comings, and open the path for new physical–chemistry device developments of oxygen sensor along with providing a guideline for innovators in oxygen sensing

Bi-allelic Loss-of-Function CACNA1B Mutations in Progressive Epilepsy-Dyskinesia.

The occurrence of non-epileptic hyperkinetic movements in the context of developmental epileptic encephalopathies is an increasingly recognized phenomenon. Identification of causative mutations provides an important insight into common pathogenic mechanisms that cause both seizures and abnormal motor control. We report bi-allelic loss-of-function CACNA1B variants in six children from three unrelated families whose affected members present with a complex and progressive neurological syndrome. All affected individuals presented with epileptic encephalopathy, severe neurodevelopmental delay (often with regression), and a hyperkinetic movement disorder. Additional neurological features included postnatal microcephaly and hypotonia. Five children died in childhood or adolescence (mean age of death: 9 years), mainly as a result of secondary respiratory complications. CACNA1B encodes the pore-forming subunit of the pre-synaptic neuronal voltage-gated calcium channel Cav2.2/N-type, crucial for SNARE-mediated neurotransmission, particularly in the early postnatal period. Bi-allelic loss-of-function variants in CACNA1B are predicted to cause disruption of Ca2+ influx, leading to impaired synaptic neurotransmission. The resultant effect on neuronal function is likely to be important in the development of involuntary movements and epilepsy. Overall, our findings provide further evidence for the key role of Cav2.2 in normal human neurodevelopment.MAK is funded by an NIHR Research Professorship and receives funding from the Wellcome Trust, Great Ormond Street Children's Hospital Charity, and Rosetrees Trust. E.M. received funding from the Rosetrees Trust (CD-A53) and Great Ormond Street Hospital Children's Charity. K.G. received funding from Temple Street Foundation. A.M. is funded by Great Ormond Street Hospital, the National Institute for Health Research (NIHR), and Biomedical Research Centre. F.L.R. and D.G. are funded by Cambridge Biomedical Research Centre. K.C. and A.S.J. are funded by NIHR Bioresource for Rare Diseases. The DDD Study presents independent research commissioned by the Health Innovation Challenge Fund (grant number HICF-1009-003), a parallel funding partnership between the Wellcome Trust and the Department of Health, and the Wellcome Trust Sanger Institute (grant number WT098051). We acknowledge support from the UK Department of Health via the NIHR comprehensive Biomedical Research Centre award to Guy's and St. Thomas' National Health Service (NHS) Foundation Trust in partnership with King's College London. This research was also supported by the NIHR Great Ormond Street Hospital Biomedical Research Centre. J.H.C. is in receipt of an NIHR Senior Investigator Award. The research team acknowledges the support of the NIHR through the Comprehensive Clinical Research Network. The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR, Department of Health, or Wellcome Trust. E.R.M. acknowledges support from NIHR Cambridge Biomedical Research Centre, an NIHR Senior Investigator Award, and the University of Cambridge has received salary support in respect of E.R.M. from the NHS in the East of England through the Clinical Academic Reserve. I.E.S. is supported by the National Health and Medical Research Council of Australia (Program Grant and Practitioner Fellowship)

Histological correlates of gastro esophageal reflux disease in South Indian population

Background: Diagnosing gastroeosopageal reflux disease (GERD) accurately is a complex problem. This study was conducted to examine the histological findings of GERD in Indian subjects. Esophageal biopsy can be combined with pH monitoring and endoscopy to define the histological damage that occurs due to acid regurgitation. The sensitivity and specificity of the individual findings needs clarity in this clouded area in order to be of use to the pathologist.Methods: A total of 102 patients with dyspepsia were included in this study. Those with heartburn and /or regurgitation were identified as patients with GERD and those without these symptoms were treated as cases of non GERD dyspepsia. Biopsies were taken 2cm above 'Z' line in all cases. The biopsies were read by a single pathologist. Basal cell hyperplasia (BCH), neutrophilic exocytosis (NE), dilated intercellular spaces (DIS), papillary elongation (PE) and lymphoid aggregates (LA), necrosis (NEC) and eosinophilic infiltration were studied. Results: 68 patients had GERD dyspepsia and 34 had non GERD dyspepsia. The histological findings of BCH, NE, PE, DIS, LA were found to be found much more often in patients with GERD symptoms (p values ranged from 0.0001 to 0.0008). We found BCH and papillary elongation together were the most sensitive histological findings. Specificity was highest when DIS combined with NE.Conclusions: In this study we found basal cell hyperplasia is the most common histological finding, and when combined with DIS or papillary elongation enhances its sensitivity. However to exclude other causes of dyspepsia, a combination of DIS, PE and NE can be used effectively.