Microbiological quality of ready-to-eat fresh vegetables and their link to food safety environment and handling practices in restaurants

Accepted, unedited manuscript- Published on-line 22-July-2016 Pending copyediting, typesetting, and reviewing of proof, and publishing in its final form.he increased consumption of ready-to-eat salads outside homes as a result of a fast paced lifestyle, awareness on their nutritional attributes and enhanced processing technology is well documented. This study aimed to determine the microbiological quality of fresh-cut salads vegetables in small and medium sized foodservice establishments (SMEs) and to identify risk factors and handling practices through observational assessment in order to investigate if an association between microbiological quality and visual assessment (inspection) scores can be established. A total of 118 samples fresh-cut vegetable salads were collected from 50 inspected locations and analysed microbiologically, in addition to 49 swabs of knives and cutting boards. There was no statistically significant correlation between visual assessment scores and bacteriological counts on vegetables or cutting boards. Nonetheless, the consistent relationship between inspection ratings on cross-contamination and cleaning components and Listeria spp. levels was statistically significant. This study demonstrated that overall visual assessment scores would not directly reflect the safety of salad vegetables and that the significance of microbiological assessment should be considered in relation to individual inspection components. It is necessary to place effective control measures on cleaning standards and risk of cross-contamination to improve the microbiological safety of fresh salad vegetables in SME

Understanding the routes of contamination of ready-to-eat vegetables in the Middle East

publisher: Elsevier articletitle: Understanding the routes of contamination of ready-to-eat vegetables in the Middle East journaltitle: Food Control articlelink: http://dx.doi.org/10.1016/j.foodcont.2015.10.024 content_type: article copyright: Copyright © 2015 Elsevier Ltd. All rights reserved

Fermentation and antimicrobial characteristics of Lactobacillus plantarum and Candida tropicalis from Nigerian fermented maize (akamu)

This study investigated the ability of Lactobacillus plantarum strains (NGL5 and NGL7) and Candida tropicalis (NGY1) previously identified from akamu-a Nigerian fermented maize food with probiotic L. plantarum LpTx and Saccharomyces boulardii SB20 to ferment ground maize slurries based on pH, acidity, microbial biomass, levels of sugars and organic acids, and their antimicrobial activity against Salmonella enterica serovar Enteritidis NCTC 5188, Escherichia coli NCTC 11560, Bacillus cereus NCIMB 11925, Staphylococcus aureus NCTC 3750 and Listeria monocytogenes NCTC 7973 using an agar spot assay. L. plantarum strains either as single or mixed starter cultures with the yeasts had growth rates ≥0.15 h-1,with pH significantly (p≤0.05) decreased to ≤3.93 after 12 h and then to ≤3.52 after 72 h and lactic acid >84 mmol L-1. The yeasts had growth rates ≥0.18 h-1 but pH was ≥4.57 with lactic acid levels ≤20.23 mmol L-1 after 72 h in the single culture fermentation. There was no inhibition in modified MRS agar: 0.2% glucose and 0.2% glucose without Tween 80. Inhibition halos in MRS agar varied from 10.6 to 23.9 mm. S. bourladii was more inhibitory towards L. monocytogenes (8.6 mm) and B. cereus (5.4 mm ) than was C. tropicalis (1.1 and 3.3 mm for L. monocytogenes NCTC 7973 and B. cereus NCIMB 11925 respectively) (0.9 mm) in malt extract agar. This study showed that C. tropicalis was less inhibitory to the pathogens while antimicrobial activities of the L. plantarum strains were mainly due to acidity and the L. plantarum strains either as single or mixed cultures with the yeasts demonstrated strong fermentation ability, with significant decrease in pH which is vital in the choice of starter for product safety

The Impact of Waxy Wheat Flour, Inulin and Guar Gum on Post‐Prandial Glycaemic and Satiety Indices, Sensory Attributes and Shelf Life of Tandoori and Pita Breads

The purpose of this study was to develop two different functional breads (Pita and Tan-doori) supplemented with novel functional ingredients: waxy wheat flour (15%), inulin (8%) and guar gum (2%) independently and in combination. The breads were submitted to sensory characteristics, shelf life, glycaemic and satiety indices assessments. Both guar gum and inulin independently and in combination significantly (p < 0.05) decreased post‐prandial blood glucose and glycemic response of Pita (GI of guar gum bread was 55%, inulin 57%) and Tandoori (GI of guar gum bread was 57% and inulin bread was 60%) compared to the control breads (GI 100%). Moreover, the results of the area under the curve of satiety showed that the addition of functional ingredients increased satiety levels as follows: for Pita, control was 355, a combination of all ingredients was 418, inulin was 451 and guar gum was 452; for Tandoori, control was 329, a combination of all ingredients was 420, inulin was 381 and guar gum was 390. The results showed that all sensory characteristics were improved, and breads were acceptable (all obtained more than five points) when the highest proportions of ingredients were added. Similarly, the shelf life of supplemented Pita and Tandoori breads was improved with the addition of ingredients. Therefore, the functional ingredients such as inulin and guar gum can be used independently and in combination to reduce GI and increase satiety of Pita and Tandoori bread with acceptable quality and shelf life

The transfer rate of Salmonella Typhimurium from contaminated parsley to other consecutively chopped batches via cutting boards under different food handling scenarios

publisher: Elsevier articletitle: The transfer rate of Salmonella Typhimurium from contaminated parsley to other consecutively chopped batches via cutting boards under different food handling scenarios journaltitle: Food Research International articlelink: http://dx.doi.org/10.1016/j.foodres.2016.09.001 content_type: article copyright: © 2016 Elsevier Ltd. All rights reserved