Wpływ różnych czynników na poziom alfa1-antytrypsyny u żrebiąt pełnej krwi angielskiej

Acute phase proteins (APP) are an integral part of the acute phase response. Alpha 1 antitrypsin is considered to be one of the most important acute-phase protein activated by trauma, stress, or inflammatory processes. The objective of the present study was to estimate the impact of various factors (sex, month of life and sire effect) on concentration of alpha 1 antitrypsin in serum of Thoroughbred foals. A total of 624 samples, collected from 39 foals were obtained in monthly intervals from first to 16 month of life and measured by STIC method (specific trypsin inhibitory captivity). The obtained results indicated the significant impact of analyzed age periods on the AAT level. Furthermore, the variation in AAT level in analyzed periods corresponded to significant changes in foals diet and maintaining. Alpha 1 antitrypsin concentration was also affected by sire effects and sex of foals. In the most investigated age periods, the impact of sire on alpha 1 antitrypsin content in serum of his progeny has been shown. The obtained results might be useful in explanation of differences in serum AAT concentration in foals during early ontogenesis which probably is a critical period that has an influence on racing performance of young horses.Wpływ różnych czynników na poziom alfa-1-antytrypsyny u źrebiąt pełnej krwi angielskiej. Białka ostrej fazy (APP) są integralną częścią tzw.: odpowiedzi ostrej fazy na stan zapalny. Alfa-1-antytrypsyna (ATT) jest uważana za jedno najważniejszych białek ostrej fazy aktywowane przez uraz, stres lub procesy zapalne. W związku z tym celem niniejszego badania była ocena wpływu różnych czynników (płeć, miesiąc życia i ojciec) na stężenia ATT w surowicy krwi źrebiąt pełnej krwi angielskiej. Materiał do badań stanowiło 624 próbek kri, zebranych od 39 źrebiąt będących potomstwem czterech ogierów, uzyskanych w odstępach miesięcznych od pierwszego do 16 miesiąca życia. Stężenie ATT oznaczono metodą STIC. Uzyskane wyniki wskazały na znaczący wpływ wieku źrebiąt na poziom AAT. Co więcej, różnice w poziomie AAT w analizowanych okresach przypadały w okresach znaczących zmian u źrebiąt. Ponadto w większości badanych okresów, wykazano wpływ ojca na stężenie ATT u potomstwa. Uzyskane wyniki mogą być przydatne w celu wyjaśnienia różnic w koncentracji AAT w surowicy krwi u źrebiąt w okresie wczesnej ontogenezy która jest krytycznym okresem, mającym wpływ na wyniki użytkowe młodych koni

Two new separation schemes for the group isolation of rare earth elements (REE) from biological and other matrices and their determination by ICP-MS, NAA and chromatographic methods

Two new group separation schemes, based on ion exchange chromatography, for the selective and quantitative isolation of rare earth elements (REE) from accompanying elements, were devised. After checking their performance with the aid of radioactive tracers, the schemes were further used together with ICP-MS, NAA and ion exchange chromatography for the determination of Sc, Y, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb and Lu in two certified reference materials (CRMs). The results were compared with another series of analyses, where the REEs were determined directly, i.e. without pre-separation, by instrumental neutron activation analysis (INAA) and inductively coupled plasma mass spectrometry (ICP-MS). It was demonstrated that while direct INAA and ICP-MS in most instances provide reliable results for the majority of REEs, for some elements, notably Sc, Yb and Tm in the cases of ICP-MS and INAA, respectively, systematic errors occur or may potentially occur

A study on chemical composition of Baszkówka and Mt. Tazerzait chondrites

The elemental contents of two new meteorites, Baszkówka (Poland, 1994) and Mt.Tazerzait (Niger, 1991), were studied by instrumental and radiochemical neutron activation analysis (NAA), supplemented in the case of some elements by gravimetric, titrimetric, spectrophotometric and atomic absorption spectrometric measurements. In total 28 elements were determined for each meteorite. A striking similarity in the bulk chemical composition of the two meteorites was observed. In the case of Baszkówka some study of the elemental contents of the magnetic fraction and the distribution of elements between kamacite and taenite phases was also made. This study confirms that Baszkówka and Mt. Tazerzait are generally more similar to L chondrites than to any other class of chondrites. However both meteorites show also some distinctive features (e.g very low Hg content), distinguishing them from all other L chondrites known. Both meteorites may derive from a common parent body, which may be different from that typical for other chondrites

Ion exchange investigation for recovery of uranium from acidic pregnant leach solutions

The article describes studies on the separation of uranium from acid pregnant leach solutions obtained from Polish uranium ores: dictyonema shales and sandstone rocks. Ion exchange chromatography was applied for uranium sorption, using commercially available, strongly basic anion exchanger, Dowex 1. In model experiments, the influence of degree of crosslinking of Dowex 1 on the efficiency of uranium extraction was investigated. The effect of H2SO4 concentration on the breakthrough curve of uranyl ions for the Dowex 1 resins, of different crosslinking: X4, X8 and X10, was examined. Unexpectedly high increase of exchange capacity of uranium was observed in case of Dowex 1X10. This gives potential opportunity of improving the effectiveness of uranium recovery process. Applying column packed with Dowex 1X10, ‘yellow cake’ with ca. 92% yield and high purity of recovered uranium was obtained. A block diagram of the procedure for uranium and lanthanides extraction from acidic leach liquor has been proposed