79 research outputs found
Patterns and Mechanisms of Ancestral Histone Protein Inheritance in Budding Yeast
Tracking of ancestral histone proteins over multiple generations of genome
replication in yeast reveals that old histones move along genes from 3′
toward 5′ over time, and that maternal histones move up to around 400 bp
during genomic replication
Cell type-specific over-expression of chromosome 21 genes in fibroblasts and fetal hearts with trisomy 21
BACKGROUND: Down syndrome (DS) is caused by trisomy 21 (+21), but the aberrations in gene expression resulting from this chromosomal aneuploidy are not yet completely understood. METHODS: We used oligonucleotide microarrays to survey mRNA expression in early- and late-passage control and +21 fibroblasts and mid-gestation fetal hearts. We supplemented this analysis with northern blotting, western blotting, real-time RT-PCR, and immunohistochemistry. RESULTS: We found chromosome 21 genes consistently over-represented among the genes over-expressed in the +21 samples. However, these sets of over-expressed genes differed across the three cell/tissue types. The chromosome 21 gene MX1 was strongly over-expressed (mean 16-fold) in senescent +21 fibroblasts, a result verified by northern and western blotting. MX1 is an interferon target gene, and its mRNA was induced by interferons present in +21 fibroblast conditioned medium, suggesting an autocrine loop for its over-expression. By immunohistochemistry the p78(MX1 )protein was induced in lesional tissue of alopecia areata, an autoimmune disorder associated with DS. We found strong over-expression of the purine biosynthesis gene GART (mean 3-fold) in fetal hearts with +21 and verified this result by northern blotting and real-time RT-PCR. CONCLUSION: Different subsets of chromosome 21 genes are over-expressed in different cell types with +21, and for some genes this over-expression is non-linear (>1.5X). Hyperactive interferon signaling is a candidate pathway for cell senescence and autoimmune disorders in DS, and abnormal purine metabolism should be investigated for a potential role in cardiac defects
Efeito de citocininas na senescência e abscisão foliar durante o cultivo in vitro de Annona glabra L.
Yeast Nop15p is an RNA-binding protein required for pre-rRNA processing and cytokinesis
Involvement of membrane cytokinin receptors in the regulation of chloroplast genome transcription
By means of run-on transcription assay we studied the role of membrane receptors of cytokinin in the regulation of the chloroplast genes transcription in Arabidopsis thaliana. The results obtained showed differential regulation of the chloroplast genome transcription by cytokinin and the leading role of AHK3 in this process
Novel synthesis, structure, and properties of 3-aryl-4-thiono-6-cyano-7-aryliminothiopyrano[2,3-d]pyrimidines
Cytokinin-binding protein from Arabidopsis thaliana leaves participating in transcription regulation
Ethylene and cytokinin in the control of senescence in detached leaves of Arabidopsis thaliana eti-5 mutant and wild-type plants
Heat shock pre-treatment enhances the response of Arabidopsis thaliana leaves and Cucurbita pepo cotyledons to benzyladenine
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